Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof

A technology of methanol-eating bacillus and bacillus, which is applied in the field of preparation of methanol-eating bacillus and its coffee extract for tobacco, can solve the problems of various types of dominant bacteria, difficult control of production conditions, unsuitable for large-scale industrial production, etc., and achieve industrialization Conditions are easy to control, reduce miscellaneous air, increase the effect of delicate and soft feeling

Inactive Publication Date: 2013-06-19
HUBEI CHINA TOBACCO IND
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, China has begun to study and use bio-fermentation to transform tobacco extracts. For example, CN101497844A reports a method for preparing tobacco spices by using microbial fermentation coffee. Mild and taste-improving effect, but the dominant bacteria used in this method has a wide variety, and the production conditions are difficult to control in the industrial production process, so it is not suitable for large-scale industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof
  • Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof
  • Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention utilizes the microorganism Bacillus methylotrophicus (Bacillus methylotrophicus) VJ4-1 CCTCC NO: M 2012004 to bioconvert the coffee extract for tobacco and prepare the method as follows:

[0034]1) Crush the coffee beans to 10 meshes, reflux extraction with water at a mass ratio of 1:20 for 3 hours, then filter, and sterilize the filtrate at 115°C for 25 minutes;

[0035] 2) Slant culture: inoculate Bacillus methylotrophicus VJ4-1 CCTCC NO: M 2012004 on the potato dextrose agar slant medium, and culture it statically for 24 hours at 25°C and pH 10, then place it in the refrigerator;

[0036] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0037] 3) Preparation of seed culture medium: use the slant culture obtained in the above step 2) to inoculate the seed culture medium, vibrate for 12 hours at 25°C and rotate at 150 rpm, and transfer 4 times in succession to prepare the seed culture medium;

[0038] The composition of the see...

Embodiment 2

[0042] The present invention utilizes the microorganism Bacillus methylotrophicus (Bacillus methylotrophicus) VJ4-1 CCTCC NO: M 2012004 to bioconvert the coffee extract for tobacco and prepare the method as follows:

[0043] 1) Crush coffee beans to 100 mesh, reflux extraction with water at a mass ratio of 1:30 for 2 hours, filter, and sterilize the filtrate at 115°C for 25 minutes;

[0044] 2) Slant culture: inoculate Bacillus methanolivorus on the potato dextrose agar slant medium, and culture it for 36 hours at 30°C and pH 7, then place it in the refrigerator;

[0045] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0046] 3) Preparation of seed culture solution: use the slant culture obtained in the above step 2) to inoculate the seed medium, vibrate and culture at 30°C and 120 rpm for 24 hours, and make the seed culture solution after continuous transfer for 3 times;

[0047] The seed liquid medium consists of: maltose 50g / L, peptone 10g / L, sodium chl...

Embodiment 3

[0051] The present invention utilizes the microorganism Bacillus methylotrophicus (Bacillus methylotrophicus) VJ4-1 CCTCC NO: M 2012004 to bioconvert the coffee extract for tobacco and prepare the method as follows:

[0052] 1) Crush coffee beans to 200 mesh, reflux extraction with water at a mass ratio of 1:40 for 1 hour, then filter, and sterilize the filtrate at 115°C for 25 minutes;

[0053] 2) Slant culture: inoculate Bacillus methylotrophicus VJ4-1 CCTCC NO: M 2012004 on the potato dextrose agar slant medium, and culture it statically for 72 hours at 35°C and pH 5, then place it in the refrigerator ;

[0054] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0055] 3) Preparation of seed culture solution: use the slant culture obtained in the above step 2) to inoculate the seed medium, vibrate and culture at 35°C and rotate at 100 rpm for 36 hours, and make the seed culture solution after two consecutive transfers;

[0056] The seed liquid medium cons...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses bacillus methylotrophicus, which is called bacillus methylotrophicus VJ4-1 and is collected with the collection number CCTCC NO:M 2012004. The invention provides a cimicifuga foetida tobacco extract for biologically transforming bacillus methylotrophicus and an application of a tobacco coffee extract to tobacco. Coffee can be used for producing a natural tobacco spice, i.e., a tobacco coffee extract by fermenting with a microorganism strain. A method disclosed by the invention has the advantages of simple process, wide raw material source, environmentally-friendly and harmless production process and good industrial use prospect; and the obtained natural spice extract can be directly applied to cigarette flavoring, has the advantages of capability of coordinating smoke, increase in light sweet flavor, fruit flavor, smoke fineness and flexibility, reduction in simulation, reduction in impure gas and improvement on smoking flavor, and can be mixed and blended with other tobacco essences and spices for use.

Description

technical field [0001] The invention relates to the technical field of spices for tobacco, in particular to a preparation method and application of bacillus methanolate and coffee extract for tobacco. Background technique [0002] In tobacco products, the aroma and taste of tobacco are very important. Flavoring and ingredients are the key links in the cigarette process and one of the effective means to improve the product style and improve the quality of cigarette aroma. At present, flavors for tobacco can be divided into chemically synthesized flavors and natural flavors, both of which have their own limitations. In recent years, natural plant flavors have become the focus and focus of research and development because of their natural harmlessness and easy availability of raw materials. The development of plant-extracted spices has limitations due to the limited available plant resources. It is a good idea to apply microbial fermentation engineering to the biosynthesis of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B3/12C12R1/07
Inventor 魏敏罗诚浩宋旭艳李冉潘曦
Owner HUBEI CHINA TOBACCO IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap