Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf

A technology of tobacco leaves and biological characteristics, which is applied in the preparation of cocoa extract compound fermented spices, tobacco leaves, and recombined tobacco leaves. It can solve the problems of high irritation, weak smoke, and uncomfortable taste, and improve the stimulation. The effect of reducing smoke irritation and increasing roasting aroma

Active Publication Date: 2013-03-20
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the taste-absorbing defects such as heavy miscellaneous gas, high irritation, weak smoke and uncomfortable taste in the current recombined tobacco leaves, the present invention provides a preparation method of tobacco leaves and cocoa extract compound fermented spices and its application in recombined tobacco leaves

Method used

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  • Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf
  • Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf

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Effect test

Embodiment 1

[0026] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0027] 1) Crush the tobacco leaves to 80 mesh, then mix the tobacco powder and cocoa powder according to the mass ratio of 1:2, add water with a mass ratio of 1:30 to the mixture, reflux extraction for 3 hours and then filter, repeat the reflux extraction filtrate for 3 times, and combine the filtrates , to obtain the mixed extract of tobacco leaves and cocoa, and sterilize it;

[0028] 2) Activation of strains: Inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, and culture at 25°C for 24 hours to obtain activated strains, and place them in a refrigerator at 4°C;

[0029] The slant culture medium is: maltose: 120mL, glucose: 20g / L, agar: 15g / L.

[0030] 3) Preparation of seed culture solution: Inoculate the slant culture obtained in ...

Embodiment 2

[0035] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0036] 1) Crush the tobacco leaves to 100 mesh, then mix the tobacco powder and cocoa powder in a mass ratio of 1:0.5, add water with a mass ratio of 1:50 to the mixture, reflux for extraction for 1 hour and then filter, repeat the reflux for 3 times to extract the filtrate, and combine the filtrate , to obtain the mixed extract of tobacco leaves and cocoa, and sterilize it;

[0037] 2) Activation of strains: Inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, and culture at 38°C for 72 hours to obtain activated strains, and place them in a refrigerator at 4°C;

[0038] The slant culture medium is: maltose: 120mL, glucose: 20g / L, agar: 15g / L.

[0039] 3) Preparation of seed culture solution: Inoculate the slant culture obtained in ste...

Embodiment 3

[0044] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0045] 1) Crush the tobacco leaves to 90 mesh, then mix the tobacco powder and cocoa powder in a mass ratio of 1:1, add water with a mass ratio of 1:40 to the mixture, reflux for extraction for 2 hours and then filter, repeat the reflux for 3 times to extract the filtrate, and combine the filtrate , to obtain the mixed extract of tobacco leaves and cocoa, and sterilize it;

[0046] 2) Strain activation: Inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, and culture at 28°C for 72 hours to obtain the activated strain, and place it in a refrigerator at 4°C;

[0047] The slant culture medium is: maltose: 120mL, glucose: 20g / L, agar: 15g / L.

[0048] 3) Preparation of seed culture solution: Inoculate the slant culture obtained in step 2) ...

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Abstract

The present invention discloses a tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of a tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf. According to the present invention, a name of the strain is Raoultella planticola VP4-4, a preservation number is CCTCC M 2012005, the total 16SrRNA sequence length is 1408 bp, and similarity between the 16SrRNA sequence and Raoultella planticola is 99.563%. According to the method, the Raoultella planticola VP4-4 is adopted, and bacteria seed activation, seed culture liquid preparation, fermentation culture and fermentation solution transformation are performed to obtain the tobacco leaf extract and cocoa extract composite fermentation concrete. With the present invention, the process is simple, the raw materials are the cigarette product origin tobacco leaves and cocoa perfumes capable of applying in cigarettes, and the method has an industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of recombined tobacco leaf additives, in particular to a method for preparing tobacco leaves and cocoa extract compound fermented spices and its application in recombined tobacco leaves. Background technique [0002] Reconstituted tobacco leaves, also known as reconstituted tobacco leaves or homogeneous tobacco leaves, are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. The existing domestic papermaking thin sheet production process extracts the water-soluble substances in tobacco leaves and tobacco stems with water, and after solid-liquid separation, the solid part is pulped and then made into a paper base, while the liquid part is concentrated and then coated onto the paper. basically. This production process is only a physical recombination process of raw materials to a large extent, and the chemical components of the recombined toba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B3/12A24B15/28C12R1/01
Inventor 罗诚浩魏敏陈义坤李冉罗广丰
Owner HUBEI CHINA TOBACCO IND
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