The invention discloses a preparation method of beef with 
flavor of Yi people. The preparation method comprises the following steps of: S1, removing fasciae, 
grease and impurities from beef, and chopping the beef into strips; S2, weighing 
pickling ingredients; S3, uniformly mixing the 
pickling ingredients with the beef; S4, 
pickling the beef at 0-10 DEG C for 2-3 days; S5, weighing precooking materials; S6, precooking the pickled beef; S7, boiling the precooked beef; S8, cooling the 
boiled beef; S9, cooling for determining the nature of the beef; S10, 
cutting the cooled beef into blocks; S11, 
roasting the beef blocks; S12, cooling the roasted beef blocks; S13, metering and packaging the cooled beef; S14, sterilizing the packaged beef; and S15, cooling the sterilized beef. the ingredients, such as natural spice 
Litsea pungens, produced in Yi region and a modern 
processing technology are adopted, the unique 
flavor of traditional beef jerky of Yi people is preserved, the 
processing period is shortened, the yield is improved, the production cost is lowered, the 
nutrition loss in 
processing is lowered, the guarantee period of the beef is prolonged, and the beef has the characteristics of environmental friendliness, health, rich 
nutrition, pure 
flavor of Yi People and the like.