Reduction response type carbon dot drug-loaded nanocluster coated with cell membrane, and preparation and application of reduction response type carbon dot drug-loaded nanocluster

A drug-loaded nanometer and nanocluster technology, which can be applied to preparations for in vivo experiments, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as poor tumor efficacy

Active Publication Date: 2021-02-02
DONGHUA UNIV
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  • Claims
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a reduction-responsive carbon-dot drug-loaded nanocluster coated with a cell membrane and its preparation method and application, so as to overcome the defects in the prior art that the carbon-dot drug-loaded drug is not effective in treating tumors

Method used

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  • Reduction response type carbon dot drug-loaded nanocluster coated with cell membrane, and preparation and application of reduction response type carbon dot drug-loaded nanocluster
  • Reduction response type carbon dot drug-loaded nanocluster coated with cell membrane, and preparation and application of reduction response type carbon dot drug-loaded nanocluster
  • Reduction response type carbon dot drug-loaded nanocluster coated with cell membrane, and preparation and application of reduction response type carbon dot drug-loaded nanocluster

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Experimental program
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Effect test

Embodiment 1

[0077] (1) Dissolve 0.2g of 4-aminosalicylic acid in 10mL of ultrapure water, then transfer the mixed solution to a polytetrafluoroethylene reactor for hydrothermal reaction, the reaction temperature is 180°C, and the reaction time is 3h; After the reaction, it was naturally cooled to room temperature, and the product was centrifuged for 20 min (4000 rpm) to remove excessively carbonized particles, then filtered through a microporous membrane with a pore size of 220 nm, and finally freeze-dried to obtain carbon dot nanoparticles y-CDs.

[0078](2) Dissolve 30mg y-CDs in 15mL ultrapure water, stir at room temperature, add EDC (174mg, 1mL ultrapure water) dropwise to the solution, and after 30min, add NHS (95mg, 1mL ultrapure water) dropwise to the solution water), stirred for 3 h to activate the carboxyl groups on the y-CDs. Subsequently, cystamine dihydrochloride (30 mg, 3 mL ultrapure water) solution was added to the solution, stirred for 3 days, and then dialyzed in ultrapur...

Embodiment 2

[0083] Take 1 mg each of y-CDs, y-CDs NCs / DOX, and y-CDs NCs / DOX@CCM synthesized in Example 1, dilute to 50 μg / mL with ultrapure water, and dissolve 50 μL of y-CDs NCs in 950 μL The ultrapure water was used to measure the surface potential, hydrodynamic diameter and dispersion coefficient. The results are shown in Table 1. The potential of y-CDs is -26.9mV, and the potential of y-CDsNCs obtained by crosslinking y-CDs becomes -4.5mV, the hydrated particle size increased to 236.7nm, the increase in potential and hydrated particle size proved the successful synthesis of y-CDs NCs. When y-CDs NCs were loaded with chemotherapeutic drug DOX, the potential changed from negative to positive and rose to 8.8 mV, which proved the successful loading of DOX. The y-CDs NCs / DOX@CCM obtained by physically extrusion-coating the B16 cell membrane had a potential of -11.7mV, and the hydrated particle size increased to 254.8nm, which proved the successful coating of the cell membrane on the surfa...

Embodiment 3

[0087] Get y-CDs, y-CDs NCs prepared in embodiment 1 and carry out FT-IR characterization, such as image 3 As shown, curve 1 represents y-CDs NCs, and curve 2 represents y-CDs. 3438cm in curve 2 -1 The peak at corresponds to the O-H stretching vibration absorption peak of the carboxyl group, 1606cm -1 The peak at corresponds to the stretching vibration absorption peak of C=O, 1495cm -1 The peak at corresponds to the C-O stretching vibration absorption peak, which proves that the prepared y-CDs have abundant carboxyl groups on the surface. Curve 1, 1631cm -1 and 1535cm -1 The strong absorption peak at belongs to the amide bond produced by the combination of the amino group of cystamine dihydrochloride and the carboxyl group on the surface of y-CDs; 551cm -1 Compared with curve 1, the characteristic peak at is significantly enhanced, which should be attributed to the S-S bond in cystamine dihydrochloride. The infrared spectrogram results proved the successful preparation ...

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Abstract

The invention relates to a reduction response type carbon dot drug-loaded nanocluster coated with a cell membrane, and the preparation and application of the reduction response type carbon dot drug-loaded nanocluster. The preparation method comprises the following steps: the preparation of fluorescent carbon dots, the preparation of a reduction response type carbon dot nanocluster solution, the preparation of an adriamycin-loaded carbon dot nanocluster, the preparation of a B16 cell membrane suspension, and the preparation of the reduction response type carbon dot drug-loaded nanocluster coated with the cell membrane. The method is simple in process, simple in reaction condition and easy to operate and separate, and has good development prospects. The prepared reduction response type carbon dot drug-loaded nanocluster coated with the cell membrane has remarkable anti-tumor effects after entering a mouse body through caudal vein injection, and has potential clinical application value.

Description

technical field [0001] The invention belongs to the field of responsive nano-medicine carrier and its preparation and application, in particular to a reduction-responsive carbon-dot drug-carrying nano-cluster coated with a cell membrane, its preparation method and application. Background technique [0002] At present, cancer has become a major factor threatening human survival and health. For advanced cancers, chemotherapy is the main and most effective treatment method, but traditional chemotherapy has caused strong toxic side effects on normal tissues and organs while killing cancer cells. Chemotherapy drugs used in clinical practice, such as doxorubicin and paclitaxel, have greatly reduced their curative effect due to limitations such as poor water solubility and fast metabolism. Therefore, nano-platforms constructed by loading chemotherapeutic drugs on nanomaterials can deliver targeted drugs to tumor sites, and realize the responsive release of drugs in the tumor micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/04A61K31/704A61P35/00A61K49/00
CPCA61K9/0019A61K9/5115A61K9/5176A61K9/5192A61K31/704A61K49/0019A61K49/0093A61P35/00
Inventor 沈明武郭云琦范钰史向阳
Owner DONGHUA UNIV
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