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Method for preparing manganese oxide supported hybrid sodium alga acid nanogel

A sodium alginate and nanogel technology, which is applied to preparations, pharmaceutical formulations, ointment delivery and other directions for in vivo testing, can solve problems such as no discovery, and achieve the effects of simple method, small particle size and low cost

Active Publication Date: 2018-02-16
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Retrieving domestic and foreign literature has not yet found PEI-Mn synthesized by solvothermal method 3 o 4 Related reports on the preparation of sodium alginate nanogel as a cross-linking agent as an MRI contrast agent

Method used

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  • Method for preparing manganese oxide supported hybrid sodium alga acid nanogel
  • Method for preparing manganese oxide supported hybrid sodium alga acid nanogel
  • Method for preparing manganese oxide supported hybrid sodium alga acid nanogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take 5 mL of 1 wt% AG (50 mg) aqueous solution, activate it with 88.7 mg EDC and 53.25 mg NHS for 3 h, then add dropwise to 10 mL of 2.5 wt % AOT in dichloromethane solution, stir for 30 min to form W / O emulsion, and then the W / O emulsion was added dropwise into 75 mL of 2wt% PVA aqueous solution, and stirred for 30 min to obtain a W / O / W polymer emulsion.

[0035] (2) Disperse polyethyleneimine PEI (120mg) in diethylene glycol DEG (12mL) to obtain polyethyleneimine solution, and then manganese acetylacetonate Mn(acac) 2 (422.7mg) was dispersed in polyethyleneimine solution, stirred at 50°C for 1h, then transferred to an autoclave, stirred until uniform, reacted at 180°C for 24h, cooled, centrifuged, dialyzed, cooled and dried to obtain poly Ethyleneimine PEI Modified Manganese Oxide Nanoparticles PEI-Mn 3 o 4 .

[0036] (3) Manganese oxide nanoparticles PEI-Mn modified with polyethyleneimine PEI in step (2) 3 o 4 (100mg, 5mg / mL) aqueous solution was added to th...

Embodiment 2

[0041] Get the AG / PEI-Mn that embodiment 1 prepares 3 o 4 Nanogel (1 mg), which was diluted to 50 μg / mL with ultrapure water, was used to measure surface potential and hydrodynamic diameter. Zeta potential measurement results show that AG / PEI-Mn 3 o 4 The surface potential of the nanogel is -17.8mV, and the cross-linking agent PEI-Mn 3 o 4 The surface potential of +40.7mV proves that AG and PEI-Mn 3 o 4 successful crosslinking. Its hydrodynamic diameter is 216.2nm, the particle size distribution is uniform, and the hydrodynamic diameter can remain almost unchanged for a long time ( Figure 4 ), thus indicating that AG / PEI-Mn 3 o 4 Nanogels have good colloidal stability.

Embodiment 3

[0043] Measure the AG / PEI-Mn that embodiment 1 prepares by ICP-AES test method 3 o 4 The content of Mn element in the nanogel. Prepare AG / PEI-Mn with Mn concentration of 0.016, 0.0325, 0.065, 0.13, 0.26mM respectively 3 o 4 Nanogel aqueous solution 2mL, the T of material under different Mn concentration is measured by magnetic resonance imaging analyzer 1 relaxation effects (such as Figure 5 ). The relaxation rate test results show that AG / PEI-Mn 3 o 4 The reciprocal of the relaxation time of the nanogel has a good linear relationship with the increase of Mn concentration (in the range of 0.016-0.26mM concentration). Calculate AG / PEI-Mn 3 o 4 r of nanogel 1 The value is 26.12mM -1 the s -1 , 5.7 times that of conventional Magnevist (Gd-DTPA). Therefore, the AG / PEI-Mn prepared in Example 1 3 o 4 Nanogel can be used as an excellent T in MR molecular imaging diagnosis 1 Positive contrast agent.

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Abstract

The invention relates to a method for preparing manganese oxide supported hybrid sodium alga acid nanogel. The method comprises the following steps: performing EDC / NHS activation on sodium alga acid,and performing double emulsification to obtain W / O / W polymer emulsion; adding a polyethyleneimine (PEI) modified solution of manganese oxide nano-particles PEI-Mn3O4 serving as a cross-linking agent into the emulsion, and performing chemical cross-linking, thereby obtaining the manganese oxide supported hybrid sodium alga acid nanogel. The method disclosed by the invention is simple, easy to operate and separate, low in cost, wide in raw source, low in price and biodegradable and has excellent development prospects; the prepared manganese oxide supported hybrid sodium alga acid nanogel is small in particle size and uniform in distribution, has excellent water solubility, colloidal stability and cellular compatibility, does not have any adverse effect on a living organism, has high r1 relaxation rate and excellent contrast effect and has potential application value in the field of magnetic resonance imaging contrast agents.

Description

technical field [0001] The invention belongs to the field of magnetic resonance imaging contrast agents, in particular to a preparation method of a hybrid sodium alginate nanogel loaded with manganese oxide. Background technique [0002] Magnetic resonance imaging (MRI) technology is an advanced medical imaging diagnostic technology developed in the 1970s, and has been widely used in the detection and early diagnosis of various human diseases. MRI has high resolution, high spatial and tomographic imaging capabilities, no ionization damage caused by radiation, and can obtain anatomical and physiological information, which has incomparable advantages in other medical imaging. MRI is playing an increasingly important role in the field of disease surveillance. However, the weakness of MRI is its low sensitivity, and the overlapping relaxation times of different organs or tumor tissues make MRI diagnosis difficult. In recent years, the method of injecting MRI contrast agent can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/18C08J3/075C08L29/04C08L5/04C08K9/04C08K3/22
CPCA61K49/126A61K49/1803C08J3/075C08J2329/04C08J2405/04C08K9/08C08K2003/2262C08K2201/011
Inventor 史向阳孙文杰周一伟张昌昌史梦晗彭琛
Owner DONGHUA UNIV
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