Probiotic preparation based on W1/O/W2 type dual emulsion structure, preparation method and application

Abstract

The invention belongs to the technical field of biology, and particularly relates to a probiotic preparation based on a W1 / O / W2 type dual emulsion structure, a preparation method and an application. The preparation method comprises the preparation processes of mixing probiotic thalli with a probiotic protective agent to form an inner water phase W1; enabling an oil-soluble emulsifying agent to dissolve in edible oil to form an oil phase O, performing primary mixing on the oil phase O and the inner water phase W1, performing stirring, and performing emulsifying by a low-energy emulsification method or a high energy emulsification method to obtain water-in-oil raw milk W1 / O; and using a hydrophilic emulsifier as an outer water phase W2, adding the W2 to the raw milk W1 / O, performing stirring, and performing emulsifying by the low-energy emulsification method or the high energy emulsification method to obtain W1 / O / W2 type dual emulsion, and performing low-temperature preservation to obtain the probiotic preparation. A dual emulsion system is adopted for performing anti-freezing protection entrapping on probiotics, so that the probiotics can maintain activity in the cryogenic storage process and the frost thawing process, influence of external environment on the probiotics can be reduced, and the frost thawing and storage stability of the probiotics within shelf life of products can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a W-based 1 / O / W 2 Probiotic preparation, preparation method and application of type double emulsion structure. Background technique [0002] Probiotics are a type of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects to improve the host's micro-ecological balance and exert beneficial effects. A large number of studies have shown that probiotics have a variety of physiological activities, such as: regulating intestinal health, improving the synthesis and bioavailability of nutrients in the body, lowering cholesterol levels, lowering blood pressure, alleviating tumors, preventing intestinal cancer, improving the body's immune level, etc. . [0003] Products made from probiotics, also known as probiotics, include La...

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Problems solved by technology

[0008] The present invention has developed a new type of emulsion system containing probiotics, which can be used in low-temperature product systems such as ice cream, ice cream, low-temperature yogurt, cheese, low-temperature beverages and low-temperature animal health products, and solves the problem of probiotics in this system. The problem of easy inactivation during storage improves the freeze-thaw and storage stability of probiotics, and helps probiotics to better exert their physiological activity

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