Litopenaeus vannamei water-free keep-alive transporting method

A technology for keeping alive and transporting without water, applied in fish farming, application, climate change adaptation, etc., can solve the problems of high cost, low survival rate, short keep alive time, etc., so as to reduce transportation cost and improve survival rate. , the effect of controlling transportation costs

Inactive Publication Date: 2012-06-20
3 Cites 19 Cited by

AI-Extracted Technical Summary

Problems solved by technology

Some existing anhydrous live transportation techniques and methods cannot be applied an...
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The invention relates to the aquatic culture animal living body transporting technology, in particular to a litopenaeus vannamei water-free keep-alive transporting method, which is characterized in that: adult litopenaeus vannamei is stopped eating for 1 to 2 days, then the vannamei is poured into normal-temperature water, then the temperature of the water is gradually reduced to 11 to 13 DEG C, the low-temperature condition is maintained for 10 to 30 minutes, so the litopenaeus vannamei enters a dormant state; the lodged litopenaeus vannamei is horizontally paved on wet and precooled sponge to be arranged inside a sealed bag which is filled with oxygen, the sealed bag is arranged inside an insulation can to be sealed and to be transported; and after arriving in the destination, the litopenaeus vannamei is arranged in the water with the temperature of 15 to 18 DEG C, and after the temperature is balanced, the litopenaeus vannamei is resurrected in the normal-temperature water. By adopting the method to transport the litopenaeus vannamei, the transportation time can reach 48 hours, and the survival rate can be maintained more than 95 percent.

Application Domain

Climate change adaptationPisciculture and aquaria

Technology Topic

EcologyLitopenaeus +8


  • Litopenaeus vannamei water-free keep-alive transporting method


  • Effect test(1)

Test Example

[0023] Application example:
[0024] The experiment was carried out in the Tianli Aquaculture Base of Jiangsu Tianfulai Feed Development Co., Ltd. The selected prawn species was Penaeus vannamei, the single area of ​​the breeding pond was 6m*4m*1.5m, and the prawns were commercial specifications (body length 12±1cm). The destination is Taiyuan City, Shanxi Province.
[0025] 1. Suspension of food and temporary rearing: Stop feeding the adult prawns to be transported 1 to 2 days before transportation to empty their metabolites to prevent the discharge of feces and other wastes during the later anhydrous live transportation process, on the one hand, produce ammonia nitrogen, etc. Exhaust gas kills live shrimp, on the other hand waste such as feces can reduce the quality of shrimp products.
[0026] 2. Cool down and hibernate: Put the prawns after temporary cultivation in the filtered and treated normal temperature water (requires that the water body is fresh, oxygenated, and the salinity is consistent with the shrimp when they leave the pond, and the toxic and harmful substances are not exceeded), and the cold storage is carried out through phase change. The water body is cooled step by step, and the cooling rate is controlled at 5°C/hour, and the prawns are gradually cooled down to their ecological freezing point of 11-13°C in about two hours. Minutes decreased to 40-60 times/minute, the prawns appeared to be lodging and motionless in the water body, but the breathing continued, and the abdominal feet occasionally swayed. During the period of the slowing of the breathing rate, the water body continued to increase oxygen to prevent the prawns from dying due to hypoxia under stress conditions. , if the temperature is too high, the shrimp will still be active, and if the temperature is too low, the shrimp will die.
[0027] 3. Oxygenation by bagging: put the sponge as a carrier into the water body of 11-13℃ in advance to pre-cool, and then slightly wring it out after taking it out, so as not to drip water, so as to keep a certain humidity and low temperature, and put the prawns in hibernation state. After taking it out, spread it evenly on the sponge with a moderate density, and then place it layer by layer according to the principle of layered shrimp, and three more layers can be placed. Then put it in a sealed plastic bag, and immediately oxygenate (the oxygen used is medical grade), and seal it after completion.
[0028] 4. Packing and transportation: The transport incubator used is made of foam material, the specification is 50cm*35cm*30cm, and the wall thickness is 2cm, and then a layer of temperature insulation film is placed in the transport incubator, and a phase change is placed at the bottom of the incubator and around it. Cooling material. For the phase-change cold-storage material, see the phase-change cold-storage material described in the published patent "Preparation Method of Phase-change Cold-Storage Material for Keeping and Transporting Penaeus vannamei" (Publication No.: CN101942289A), so as to maintain the low temperature in the box for a long time, and then Packed in airtight bags, each box can hold 2.5kg of shrimp. Due to the role of the phase change cold storage material and the transformation of the heat preservation box, the temperature during transportation can be maintained at about 10 to 15 °C to the greatest extent. After packing, it will be transported, and the transport time should be controlled within 48 hours.
[0029] 5. Warming up and resurrection: After the incubator is transported to the destination, take out the sealed bag and place it in a water body of 15-18 °C, so that the temperature in the bag is gradually increased by the exchange of the water body. Take out the prawns and pour them into the room temperature water to revive them. It can be seen from the observation that the swimming frequency of the gastropods of the prawns gradually increased, the breathing frequency gradually increased, and they started to swim after being resurrected.


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