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Probiotic liquid preparation based on double-emulsion structure and preparation method thereof

A probiotics, double emulsion technology, applied in the directions of emulsion delivery, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc. The effect of improving storage stability and extending shelf life

Active Publication Date: 2018-11-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has developed a new type of liquid delivery system loaded with probiotics. While fully ensuring the probiotic effect of probiotic preparations, it solves the problem that probiotics are easily inactivated during normal temperature storage and body digestion, and improves the oral administration of probiotics. The stability of digestion is conducive to the better physiological activity of probiotics in the body

Method used

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  • Probiotic liquid preparation based on double-emulsion structure and preparation method thereof
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  • Probiotic liquid preparation based on double-emulsion structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 A preparation method of probiotic liquid preparation based on double emulsion structure

[0059] 1, a kind of based on double emulsion W 1 / O / W 2 The preparation method of the probiotic liquid preparation of structure comprises the following steps:

[0060] (1) Preparation of bacterial suspension: Activate the probiotic Lactobacillus acidophilus with MRS broth medium, culture in a constant temperature incubator at 37°C for 12 to 18 hours, and then centrifuge the bacterial liquid to obtain bacterial cells. Suspended in sterile water to obtain a bacterial suspension;

[0061] (2) Inner water phase W 1 Preparation: pre-dissolve chitosan with 1% acetic acid solution to obtain 0.5wt% chitosan solution; adjust its pH value to neutral with 0.5mol / L sodium hydroxide solution, add 9log / mL Lactobacillus acidophilus 0.5wt% sodium alginate solution, after mixing, add 1wt% divalent calcium ion to induce, so that it forms a gel-like inner water phase W 1 ;

[0062] (3...

Embodiment 2

[0066] Implementation effect of embodiment 2

[0067] 1. Method:

[0068] Take the Lactobacillus acidophilus suspended in the buffer as the control group, embedded in the double emulsion W of embodiment 1 1 / O / W 2 Lactobacillus acidophilus in is the experimental group. Lactobacillus acidophilus was suspended in the external aqueous phase of different pH values ​​(pH 1.5, 5.7, 7.2, 9.0), and embedded in the double emulsion W of Example 1 1 / O / W 2 The Lactobacillus acidophilus in the medium was mixed with the external water of different pH values ​​(pH 1.5, 5.7, 7.2, 9.0) in equal proportions, and the effects of different pH values ​​on the double emulsion W 1 / O / W 2 Effect of Lactobacillus acidophilus. For the treatment groups with pH values ​​of 1.5 and 9.0, the number of live bacteria was measured by dilution coating plate method at 0, 1, 2 and 3 hours after mixing, and the survival rate was calculated. For the treatment groups with pH value of 5.7 and 7.2, the number ...

Embodiment 3

[0082] Example 3 A preparation method of probiotic liquid preparation based on double emulsion structure

[0083] 1, a kind of based on double emulsion W 1 / O / W 2 The preparation method of the probiotic liquid preparation of structure comprises the following steps:

[0084] (1) Preparation of bacterial suspension: activate the probiotic Bifidobacterium longum with MRS broth medium, culture in a constant temperature incubator at 37°C for 12 to 18 hours, and then centrifuge the bacterial liquid to obtain bacterial cells. Suspended in sterile water to obtain a bacterial suspension;

[0085] (2) Inner water phase W 1 Preparation: pre-dissolve chitosan with 1% acetic acid solution to obtain 2wt% chitosan solution; adjust its pH value to neutral with 0.5mol / L sodium hydroxide solution, add 9log / mL Bifidobacterium longum 0.01wt% sodium alginate in the suspension, after mixing, add 0.01wt% divalent calcium ion to induce it to form a gel-like inner water phase W 1 ;

[0086] (3) ...

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Abstract

The invention discloses a probiotic liquid preparation based on a double-emulsion structure and a preparation method thereof. The method comprises the following steps that S1, a probiotic bacterium suspension, a thickener and an intestinal tract auxiliary release material (namely a material capable of assisting in the release of probiotics in an intestinal tract) are mixed, and induction is carried out with bivalent salt ions to form a gel-like inner water phase W1; S2, an oil-soluble emulsifier is dissolved in edible oil to form an oil phase O, and the oil phase O and the inner water phase W1are subjected to high-speed shearing and mixing so as to obtain a water-in-oil type primary emulsion W1 / O; and S3, a suspension of colloidal particles is served as an outer water phase W2, the outerwater phase W2 is added into the primary emulsion W1 / O, and intermediate-speed shearing and mixing are carried out so as to obtain the probiotic emulsion preparation based on the W1 / O / W2 type double-emulsion structure. According to the probiotic liquid preparation and the preparation method thereof, protective embedding is carried out on probiotics by adopting a Pickering double-emulsion system, so that the probiotic effect is fully guaranteed, meanwhile, the probiotics can be prevented from being damaged by oxygen and the environment of a gastrointestinal tract, and therefore the action of gastric acid and cholate can be resisted, in addition, colonization can be better carried out in the intestinal tract, and a wide application prospect is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a probiotic liquid preparation based on a double emulsion structure and a preparation method thereof. Background technique [0002] Probiotics are live microorganisms that, when administered in adequate doses, have a beneficial effect on the health of the host. It hosts host cells, promotes the intestinal microecological balance of the host, and has beneficial effects on the health and / or physiological functions of the host, such as improving gastrointestinal function, regulating intestinal flora, lowering cholesterol, improving blood lipids, and enhancing immunity wait. Products made from probiotics are commonly referred to as microecological preparations, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus bulgaricus ( Lactobacillus bulgaricus), Bifidobacterium, Bifidobacterium bifidum, Bifidobacterium adolescentis and Strepto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/113A61K47/36A61K47/32A61K35/741A61K35/747A61P1/00
CPCA61K9/113A61K35/741A61K35/747A61K47/32A61K47/36A61P1/00
Inventor 肖杰王玲王文博罗敏娜陈霞
Owner SOUTH CHINA AGRI UNIV
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