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Hyaluronic acid-gelatin composite hydrogel carrying PLGA microspheres and preparation method thereof

A technology of composite hydrogel and hyaluronic acid, which is applied in the fields of pharmaceutical formulation, medical science, drug delivery, etc., can solve the problems of patients with harmful side effects, and achieve excellent swelling properties, slow release of antibacterial properties of drugs, and low mechanical properties.

Inactive Publication Date: 2020-06-05
海南卓瑞生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrogels proved to have the disadvantage of relatively fast release profiles, causing harmful side effects to patients due to exposure to high drug concentrations

Method used

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  • Hyaluronic acid-gelatin composite hydrogel carrying PLGA microspheres and preparation method thereof
  • Hyaluronic acid-gelatin composite hydrogel carrying PLGA microspheres and preparation method thereof
  • Hyaluronic acid-gelatin composite hydrogel carrying PLGA microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A hyaluronic acid-gelatin composite hydrogel loaded with PLGA microspheres, the hydrogel comprises the following components in mass percent: 12.5% ​​aminoethyl methacrylate hyaluronic acid, 10% methacrylated gelatin, PLGA@GS composite microspheres 0.5%, photoinitiator 0.1%, and water as the balance.

[0037] The preparation method of the hydrogel in this example is: 0.25g HA-AEMA (12.5wt.%), 0.2g GelMA (10wt.%), 10mg PLGA@GS microspheres (0.5wt.%) and 2mg LAP photoinitiated HA-AEMA / GelMA / PLGA@GS hydrogel was obtained by dissolving GelMA (0.1wt.%) in 2mL deionized water and irradiating with ultraviolet light for 20min.

[0038] Among them, the preparation method of aminoethyl methacrylate hyaluronic acid is: weigh 1g hyaluronic acid and dissolve it in 100mL distilled water, after the hyaluronic acid is completely dissolved, add 0.25g EDC, 0.125g NHS, 0.125g AEMA, and then React at room temperature for 24 hours, dialyze with a cellulose dialysis bag with a molecular weig...

Embodiment 2

[0042] A hyaluronic acid-gelatin composite hydrogel loaded with PLGA microspheres, the hydrogel comprises the following components in mass percent: 1% aminoethyl methacrylate hyaluronic acid, 20% methacrylated gelatin, 0.01% of PLGA@GS composite microspheres, 0.01% of photoinitiator, and the balance is water.

[0043] The preparation method of the hydrogel in this example is as follows: 0.01g HA-AEMA (1wt.%), 0.4g GelMA (20wt.%), 0.2mg PLGA@GS microspheres (0.01wt.%) and 0.2mg LAP light The initiator (0.01wt.%) was dissolved in 2 mL of deionized water, and irradiated with ultraviolet light for 20 min to obtain the HA-AEMA / GelMA hydrogel.

[0044] The preparation method of aminoethyl methacrylate hyaluronic acid, methacrylated gelatin, and PLGA@GS composite microspheres is the same as in Example 1.

Embodiment 3

[0046] A hyaluronic acid-gelatin composite hydrogel loaded with PLGA microspheres, the hydrogel comprises the following components in mass percent: 20% aminoethyl methacrylate hyaluronic acid, 1% methacrylated gelatin, PLGA@GS composite microspheres 1%, photoinitiator 0.5%, and water as the balance.

[0047] The preparation method of the hydrogel in this embodiment is: 0.2g HA-AEMA (20wt.%), 0.02g GelMA (1wt.%), 20mg PLGA@GS microspheres (1wt.%) and 10mg LAP photoinitiator ( 0.5 wt.%) was dissolved in 2 mL of deionized water, and irradiated with ultraviolet light for 20 min to obtain HA-AEMA / GelMA hydrogel.

[0048] The preparation method of aminoethyl methacrylate hyaluronic acid, methacrylated gelatin, and PLGA@GS composite microspheres is the same as in Example 1.

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Abstract

The invention relates to hyaluronic acid-gelatin composite hydrogel carrying PLGA microspheres. The hydrogel includes, by mass percentage, 1-20% of aminoethyl methacrylate hyaluronic acid, 1-20% of methacrylate gelatin, 0.01-1.0% of PLGA@GS composite microspheres, and 0.01-0.5% of a photoinitiator, with the balance being water. The hydrogel simultaneously carries the PLGA@GS microspheres, HA-AEMAand GelMA, so that the hydrogel can show a three-dimensional microporous structure and has the abilities of slowly releasing drugs and long-term antibacterial properties; the hydrogel shows excellentswelling and mechanical properties and low cytotoxicity, and has the effects of rapid hemostasis; and the hydrogel is the potential materials of hemostatic and anti-infective materials in wound dressing application.

Description

technical field [0001] The invention relates to a hyaluronic acid-gelatin composite hydrogel loaded with PLGA microspheres and a preparation method thereof, belonging to the technical field of medical biomaterials. Background technique [0002] Wound healing is a complex dynamic process involving hemostasis, inflammation, proliferation and remodeling. During the healing process, uncontrolled bleeding and wound infection are causes of delayed healing and even death. On the one hand, hemostasis is crucial and should be achieved quickly because the bleeding will occur within a short time; on the other hand, the infection will cause severe tissue damage because the microorganisms can compete with the host immune system and subsequently invade living tissue, Especially Staphylococcus aureus. Materials such as bandages, fibrin glue, liquids, and powders are usually used to stop the bleeding. Among the aforementioned materials, dressings can cover an injured site to prevent infe...

Claims

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Application Information

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IPC IPC(8): A61L26/00C08B37/08C08H1/00
CPCA61L26/0019A61L26/0023A61L26/0038A61L26/0061A61L26/0066A61L26/008A61L2300/406A61L2300/602A61L2400/04C08B37/0072C08H1/00C08L5/08C08L67/04
Inventor 马小平陈华奇
Owner 海南卓瑞生物医药有限公司
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