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rhBMP-2-loaded bone repair material microsphere and preparation method thereof

A technology of rhbmp-2 and bone repair, which is applied in the field of regenerative medicine and biomedical materials, can solve the problems that bones cannot provide good therapeutic effects, ectopic bone formation, and cannot provide concentration at the target site, etc., to achieve burst release and sustained Effect of controlled drug release, less chance of degradation reactions

Active Publication Date: 2019-12-03
ZHEJIANG RISING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, common rhBMP-loaded bone repair materials tend to release rhBMP-2 intensively in the early stage of product use, which cannot control the continuous and small amount of rhBMP-2 release, resulting in the enrichment of rhBMP-2 in local tissues for a period of time. flow or interstitial fluid to tissues other than the area of ​​tissue to be repaired, which may lead to unwanted ectopic bone formation
In addition, rhBMP-2 has a short half-life in the body, and is quickly metabolized and absorbed when used locally, making it difficult to play a role. Even if it is administered in large doses continuously, it often cannot provide a sustained and effective concentration to the target site. For long-term growth and It cannot provide good therapeutic effect for the restored bone, and it is easy to cause patients with low bone quality, inflammatory response and other potential risks

Method used

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  • rhBMP-2-loaded bone repair material microsphere and preparation method thereof
  • rhBMP-2-loaded bone repair material microsphere and preparation method thereof
  • rhBMP-2-loaded bone repair material microsphere and preparation method thereof

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Embodiment 1

[0018] Embodiment 1: Preparation of bone repair material microspheres

[0019] Natural mussel mucin extract product: produced by BD Bioscience, labeled as blue mussel product, containing Mefp-2, Mefp-2, Mefp-3;

[0020] BMP-2 is produced by the company itself, and the specific production process refers to the production method in the applicant's Chinese patent application CN201910030649.0 (expression in E. coli, purification by ion exchange chromatography, detection of E. coli host residual protein <0.005%), antibacterial Circle method to detect antibiotic residues <0.1ppm;

[0021] Polyethylene lactide is produced by DURECT Company of the United States.

[0022] Preparation:

[0023] (1) Preparation of PLGA microspheres loaded with rhBMP-2

[0024] Prepare PLGA microspheres loaded with rhBMP-2 by W / O / W method, dissolve rhBMP-2 lyophilized powder in acetic acid buffer solution, then add acetonitrile containing PLGA, emulsify at 3000rpm for 30 seconds, add liquid paraffin an...

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Abstract

The invention belongs to the field of biomedical materials containing active proteins and the field of regenerative medicine. The invention provides a bone repair material, which comprises rhBMP-2 (recombinant bone morphogenetic protein-2) and PLGA microspheres, rhBMP-2 is wrapped in the PLGA microspheres, and the surfaces of the microspheres are modified with mussel mucoprotein.

Description

technical field [0001] The invention belongs to the field of biomedical materials containing active protein and the field of regenerative medicine. Specifically, the application provides a rhBMP-2-loaded bone repair material microsphere and a preparation method thereof. Background technique [0002] The treatment of orthopedic disorders often requires bone grafting as a bridge for new bone growth, such as bone tumors, nonunion, cervical disc herniation, osteoporotic fractures of the spine, and many spinal disorders that require fusion surgery. Every year in my country, tens of thousands of patients have bone defects caused by trauma, infection, tumor and other lesions, and hundreds of thousands of patients undergo spinal fusion surgery, both of which require bone transplantation. There are many problems in bone grafting such as new surgical trauma, limited source, immune rejection, disease transmission and high cost. With the development of material science, it has become a...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/18A61L27/227A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/414A61L2430/02C08L89/00C08L67/04
Inventor 陈春曹建新
Owner ZHEJIANG RISING BIOTECH CO LTD
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