Preparation technique of swine influenza DNA vaccine PLGA microspheres

A DNA vaccine, PLGA technology, applied in the research field of drug sustained-release dosage forms, can solve the problems of short duration, easy to be degraded, unable to achieve sustained release, etc., to maintain activity, prolong the time of drug action, and sample requirements less effect

Inactive Publication Date: 2009-05-06
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Ordinary DNA vaccines are characterized by their short duration of action and are e

Method used

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Embodiment 1

[0015] The preparation technology of porcine influenza DNA vaccine PLGA microsphere, its composition comprises: the making of colostrum, double milk, and described colostrum is made as absorbing the aqueous solution of porcine influenza DNA vaccine that contains DNA weight fraction and is 2 as internal water phase, takes PLGA with 200 parts by weight is added with 5 parts by volume of dichloromethane to dissolve completely, and as the oil phase, the inner water phase is added to the oil phase, and ultrasonically emulsified for 10 seconds under ice bath conditions, To obtain colostrum, under the rotating speed of 7000rpm, the gained colostrum is added to 2% polyvinyl alcohol with a volume fraction of 20, and the emulsifier stops after stirring for 35 seconds to obtain double milk, and the gained double milk is transferred to a beaker, and Magnetic stirring was carried out at 200rpm for 5 hours, the organic solvent was evaporated at room temperature, centrifuged at 4000rpm for 10...

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Abstract

The invention relates to a preparation process of swine influenza DNA vaccine PLGA microspheres, which solves the problems that common DNA vaccines are short in function lasting time, easy to be degraded in bodies and cannot realize the aim of sustained release. The preparation process of the swine influenza DNA vaccine PLGA microspheres comprises the steps as follows: colostrum is taken to prepare multiple emulsion, and the multiple emulsion is transferred into a beaker and magnetically stirred for 5 hours, and then an organic solution is evaporated at the room temperature condition, and the centrifugal process is carried out for 10 minutes, and the microspheres are collected and washed by sterile water, and the swine influenza DNA vaccine PLGA microsphere can be obtained by the vacuum freezing and drying. The process can be used in the preparation of the swine influenza DNA vaccine PLGA microspheres.

Description

Technical field: [0001] The invention relates to a preparation process of swine influenza DNA vaccine PLGA microspheres, which belongs to the research on drug slow-release dosage forms. Background technique: [0002] Swine influenza is an acute and contagious respiratory disease of pigs caused by influenza A virus of Orthomyxoviridae. It spreads rapidly and often causes outbreaks of acute respiratory diseases. Because there are many subtypes of swine influenza virus, antigens are easy to mutate and can be transmitted between species, it brings many challenges to the prevention of influenza diseases and even human health. Therefore, swine flu plays a key role between human flu and bird flu, and has great public health significance. [0003] DNA vaccine is to directly inoculate the body with the eukaryotic expression plasmid containing the gene encoding the antigen, and express the corresponding antigen in the body to stimulate the body to generate an immune response against ...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K9/16A61K9/52A61K47/34A61K47/46A61P31/16
Inventor 赵凯金媛媛孙庆申魏海霞周东坡
Owner HEILONGJIANG UNIV
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