Blank PLGA microspheres and preparation method thereof

A blank and microsphere technology, applied in medical science, prosthesis, etc., can solve the problems of destroying the original microbial environment of the intervertebral disc, destroying the natural closed microenvironment of the intervertebral disc, and poor biological tissue compatibility, etc., to achieve the degraded microenvironment Metabolism, does not affect the microenvironment metabolism, the effect of high repetition rate

Inactive Publication Date: 2015-03-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when these cell scaffolds are used as carriers for transplanting cells, they need to be implanted into the intervertebral disc through an open incision, which destroys the natural closed microenvironment of the intervertebral disc, so the defects are obvious
At the same time, such cell scaffolds mostly use agar, hyaluronic acid, etc. as raw materials. Although they are harmless to organisms, they have destroyed the original microbial environment of the intervertebral disc due to their large size. Biodegraded after completion

Method used

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  • Blank PLGA microspheres and preparation method thereof
  • Blank PLGA microspheres and preparation method thereof
  • Blank PLGA microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1 makes blank PLGA microspheres with 6gPLGA

[0012] The preparation steps of the blank PLGA microspheres: PLGA (6g) was dissolved in 30ml of dichloromethane, and the mixed solution was injected into 300ml of 2% (w / v) polyvinyl alcohol (poly(vinyl alcohol, PVA ) aqueous solution, stirred with a magnetic stirrer at 600rpm for 2-3 hours (35°C), removed dichloromethane, centrifuged at 1500rpm for 2min, collected microspheres, washed with distilled water for 6 times, and then freeze-dried to obtain.

Embodiment 2

[0013] Example 2 Blank PLGA microspheres were made with 12g PLGA.

[0014] The preparation steps of the blank PLGA microspheres: PLGA (12 g) was dissolved in 60 ml of dichloromethane. The mixed solution was poured into 600 ml of an aqueous solution containing 2% (w / v) PVA. Stir with a magnetic stirrer at 600 rpm for 2-3 hours (35°C) to remove dichloromethane. The microspheres were collected after centrifugation at 1500 rpm for 2 min, washed with distilled water for 6 times, and then freeze-dried. (See the morphology under SEM figure 1 )

Embodiment 3

[0015] Example 3 Blank PLGA microspheres were made with 24g PLGA.

[0016] The preparation steps of the blank PLGA microspheres: PLGA (24 g) was dissolved in 120 ml of dichloromethane. The mixed solution was poured into 1200 ml of an aqueous solution containing 2% (w / v) PVA. Stir with a magnetic stirrer at 600 rpm for 2-3 hours (35°C) to remove dichloromethane. The microspheres were collected after centrifugation at 1500rpm for 2min, washed 6 times with distilled water and freeze-dried.

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Abstract

The invention provides blank polylactic acid-glycolic acid copolymer microspheres which are prepared by a water-oil-water emulsion process. The PLGA microspheres are prepared from PLGA powder by using excellent dissolving capacity of dichloromethane and emulsification of polyvinyl alcohol. Through representation observation and particle size determination of the microspheres, the diameter and the surface sign of a biological scaffold material are obtained; and the diameters of the PLGA blank microspheres accord with the injection size. Through CCK-8 determination and lactic dehydrogenase determination, the microspheres have no toxicity on attached mesenchymal stem cells, and do not affect the growth activity. Compared with chitosan particles, the microspheres are simple in preparation method, high in repetitive rate, clear structure, and easy to degrade in a living body, do not affect microenvironment metabolism, and can used as good cell carriers for different treatment targets in tissue regeneration engineering.

Description

technical field [0001] The invention belongs to biological tissue engineering cell scaffold materials, and relates to a biological material emulsification transformation method, in particular to a blank PLGA (polylactic acid-glycolic acid copolymer) microsphere and a preparation method. Background technique [0002] Many research groups have used nucleus pulposus cells or mesenchymal stem cell composite cell scaffolds for intervertebral disc tissue engineering research. In the evaluation of bioscaffold materials, solid-state and preformed cell scaffolds including mesh scaffolds and fibrous meshes are mainly used. However, when these cell scaffolds are used as carriers for transplanting cells, they need to be implanted into the intervertebral disc through an open incision, which destroys the natural closed microenvironment of the intervertebral disc, so the defects are obvious. At the same time, such cell scaffolds mostly use agar, hyaluronic acid, etc. as raw materials. Alt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/14C08L67/04A61L27/18A61L27/50
Inventor 梁成振周校澎李方财陈其昕
Owner ZHEJIANG UNIV
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