Method for preparing PLGA microspheres with porous surfaces

A technology with porous surface and microspheres, which is applied in the fields of medical science and prostheses, can solve the problems such as hard-to-form pores, achieve good sphericity, uniform shape and structure, and shorten the emulsification time

Inactive Publication Date: 2016-06-15
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, the surface of the microspheres prepared by this method is mostly micron-scale, and it is diff

Method used

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  • Method for preparing PLGA microspheres with porous surfaces
  • Method for preparing PLGA microspheres with porous surfaces

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preparation example Construction

[0027] Such as figure 1 Shown, the surface porous PLGA microsphere preparation method provided by the invention comprises:

[0028] S110: Add PLGA to the organic solvent, stir to dissolve, then drop into the porogenic solution, and ultrasonically emulsify to form an emulsion; wherein, the molar ratio of LA and GA of the PLGA ranges from 50:50 to 90:10 ;

[0029] S120: Add the emulsion to the stirred external water phase drop by drop, and add a preset volume of deionized water, change the speed and continue stirring until the organic solvent is completely volatilized, wherein the external water phase is a water-soluble surfactant added to remove ionized water;

[0030] S130: Centrifuge the solution after the organic solvent is completely volatilized, wash it with deionized water, remove the supernatant, and obtain PLGA microspheres;

[0031] S140: adding the NaOH solution into the PLGA microspheres, mixing evenly, and putting it on a shaker to continue the reaction;

[0032...

example 1

[0041] This embodiment takes the following steps:

[0042] 1) Add 100mg of PLGA (50:50) into 10mL of dichloromethane, and keep stirring until it is completely dissolved;

[0043] 2) Prepare 1wt% PVA solution (Mw=80000), draw 1mL PVA solution with a pipette gun, and add it dropwise to the oil phase in step 1) under the condition of ultrasonic intensity of 24W to form a water-in-oil emulsion , ultrasonic time 60s;

[0044] 3) Add the emulsion obtained in step 2) into 30mL of PVA aqueous solution under stirring, the stirring rate is 800rpm, after stirring for 30min, add 300mL of deionized water, change the stirring rate to 400rpm, and continue stirring for 4h until Dichloromethane is completely volatile.

[0045] 4) The PLGA microspheres were collected by centrifugation, and washed three times with deionized water at a rotation speed of 4500 rpm for 10 min.

[0046] 5) Add NaOH with a mass volume ratio of 1% and a concentration of 0.2M to the PLGA microspheres obtained in step...

example 2

[0049] This embodiment takes the following steps:

[0050] 1) Add 80mg of PLGA (65:35) into 12mL of chloroform, and keep stirring until it is completely dissolved;

[0051] 2) Prepare a 1wt% BSA solution, absorb 1mL of the BSA solution with a pipette gun, and add it dropwise to the oil phase in step 1) under the condition of an ultrasonic intensity of 32W to form a water-in-oil emulsion, and the ultrasonic time is 50s;

[0052] 3) Add the emulsion obtained in step 2) into 30mL PVA 1wt% aqueous solution under stirring, the stirring rate is 800rpm, after stirring for 30min, add 300mL deionized water therein, change the stirring rate to 400rpm, continue stirring for 4h, until the chloroform is completely evaporated.

[0053] 4) The PLGA microspheres were collected by centrifugation, and washed three times with deionized water at a rotation speed of 4500 rpm for 10 min.

[0054] 5) Add NaOH with a mass volume ratio of 1% and a concentration of 0.2M to the PLGA microspheres obtai...

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Abstract

The invention provides a method for preparing surface porous PLGA microspheres, comprising: adding PLGA to an organic solvent, stirring and dissolving, adding dropwise a pore-forming solution, and supersonic emulsification to form an emulsion; wherein, the LA of PLGA and the content of GA The molar ratio range is: 50:50~90:10; the emulsion is added dropwise to the stirred external water phase, and a preset volume of deionized water is added, and the speed is changed to continue stirring until the organic solvent is completely volatilized. Among them, The external water phase is a water-soluble surfactant added to deionized water; the solution after the organic solvent is completely volatilized is centrifuged, and washed with deionized water, and the supernatant is removed to obtain PLGA microspheres; NaOH solution is added to the PLGA microspheres. spheres, mixed evenly, and placed on a shaker to continue the reaction; the reacted PLGA microspheres were repeatedly centrifuged and washed with deionized water, and freeze-dried to obtain PLGA microspheres with porous surfaces. The invention has simple process and short period, and is suitable for industrial large-scale production.

Description

technical field [0001] The present invention relates to the technical field of polymer porous microsphere materials, and more specifically, relates to a method for preparing an injectable cell microsphere carrier with a macroporous structure on the surface by using the W / O / W double emulsification solvent volatilization method combined with directional hydrolysis technology . Background technique [0002] Polylactic acid-glycolic acid (PLGA) is a biodegradable biopolymer, and its biomechanics and degradability can be controlled by the ratio and molecular weight of its polymerized monomers lactic acid (LA) and glycolic acid. Due to its good biocompatibility, it has been approved by the US FDA for clinical use and has a wide range of applications in the field of biomedical engineering. At present, it has been made into various bioabsorbable bone screws, surgical sutures, drug carriers and tissues. Engineering support materials. Making it into microspheres with controllable mo...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/38A61L27/56
CPCA61L27/18A61L27/38A61L27/56
Inventor 李波易忠超王婧琳肖文谦刘雪杨晓玲徐文峰廖晓玲
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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