RAPA-PLGA scaffold

A RAPA-PLGA, peripheral nerve injury technology, applied in pharmaceutical science, pharmaceutical formulations, bulk delivery, etc., to avoid side effects, shorten time, and avoid iatrogenic damage.

Inactive Publication Date: 2016-11-16
丁坦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main problem at present is: under normal circumstances, the peripheral nerves are not in contact with body fluid...

Method used

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  • RAPA-PLGA scaffold
  • RAPA-PLGA scaffold
  • RAPA-PLGA scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] Embodiment of the present invention is described below, and embodiment does not constitute limitation of the present invention:

[0038] Preparation of RAPA-PLGA sustained-release microspheres:

[0039] PLGA microspheres loaded with RAPA were prepared by solvent evaporation method. Dissolve 2g of PLGA in 20ml of dichloromethane, add 200mg of RAPA at a ratio of 1:10 (w / w) to the medicinal material, shake and mix well. Add the above oil phase dropwise to 0.5% PVA (Polyvinylalcohol) solution, under the action of a high-pressure homogenizer, homogenize at 3000rpm for 5min to obtain an O / W emulsion, continue stirring at 400rpm at room temperature for 4h to evaporate the organic solvent, 3000rpm The obtained microspheres were collected by centrifugation for 5 minutes, washed with water three times, and freeze-dried to obtain RAPA-PLGA sustained-release microspheres.

[0040] RAPA-PLGA Scaffold Preparation:

[0041] Weigh 70mg and 280mg of chitosan and type I collagen respe...

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Abstract

The invention relates to the field of medicine, in particular to an RAPA-PLGA scaffold. RAPA-loaded PLGA microspheres are prepared through a solvent evaporation method, then RAPA-PLGA slow-release microspheres can be constructed, and the microspheres are added into the scaffold in the construction process of the nerve scaffold. RAPA can be locally and continuously released after the RAPA-PLGA scaffold is implanted in vivo in the early injury stage, not only secondary injuries caused by an injured nerve immunological rejection reaction are relieved, but also obstruction of scar tissue on nerve regeneration is reduced, and meanwhile the side effects of systemic administration are avoided; the purposes of relieving the injured nerve secondary injuries and promoting regeneration of peripheral nerves are achieved.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a RAPA-PLGA bracket. Background technique [0002] The repair and replacement of long-segment peripheral nerve defects is a difficult problem that often needs to be faced in clinical work. In recent years, great progress has been made in the design and construction of tissue-engineered peripheral nerve scaffolds using tissue engineering methods. Studies have shown that highly bionic peripheral nerve tissue engineering scaffolds can indeed provide temporary scaffolds for long-segment defect nerves, and guide nerve regeneration axons to crawl until they cross the defect stage. [0003] The main problem at present is: under normal circumstances, the peripheral nerves are not in contact with body fluids and blood because of the blood-nerve barrier, and are in a state of immune immunity like the central nervous system. When the peripheral nerve is injured or broken, the nerve stump and damag...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/24A61L27/20A61L27/54A61L27/50A61L27/58A61K31/436A61K9/16A61K47/34C08K5/1545
CPCA61L27/18A61K9/0002A61K9/1641A61K31/436A61L27/20A61L27/24A61L27/50A61L27/54A61L27/58A61L2300/412A61L2300/602A61L2300/604A61L2430/32C08K5/1545C08L67/04C08L5/08
Inventor 丁坦王哲张传健
Owner 丁坦
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