Preparation method of PEI-CDs (polyetherimide-carbon dots) capable of emitting orange fluorescence

A fluorescence emission, orange technology, applied in the field of preparation of orange fluorescence emission carbon dots, can solve the problems of time-consuming, complicated operation, low yield, etc., and achieve the effect of low drying cost, high cell safety, and high yield

Active Publication Date: 2019-12-13
NANJING MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of preparation method of LWE-CDs for above-mentioned problem, explore the aftertreatment method of LWE-CDs, thereby improve existing method time-consuming, complex operation, low yield and other defects

Method used

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  • Preparation method of PEI-CDs (polyetherimide-carbon dots) capable of emitting orange fluorescence
  • Preparation method of PEI-CDs (polyetherimide-carbon dots) capable of emitting orange fluorescence
  • Preparation method of PEI-CDs (polyetherimide-carbon dots) capable of emitting orange fluorescence

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preparation example Construction

[0030] The preparation method of orange fluorescent emission PEI-CDs, the steps are as follows:

[0031] (1) Weigh TMB and PEI into a centrifuge tube, add ultrapure water, sonicate until completely dissolved, transfer to an autoclave, and react at 100-140°C for 1-4 hours to obtain a dark brown stock solution;

[0032] The concentration of TMB in the reaction system is 0.015-0.150 mol / L;

[0033] The concentration ratio of TMB to PEI is 1:1~10:1.

[0034] (2) Cool the raw solution to room temperature, transfer it to a dialysis bag with a molecular weight cut-off of 1000, and dialyze it for 6 h, then use a rotary evaporator to rotary evaporate the obtained dialysate to 2 mL at 60 °C. Then, to purify the dialysate, first add an appropriate amount of absolute ethanol, then add petroleum ether dropwise, precipitate, centrifuge, discard the supernatant to obtain PEI-CDs precipitate, and wash the precipitate 3 times with petroleum ether. Finally, the precipitate was vacuum-dried at...

Embodiment 5-9

[0039] As can be seen from Examples 5-9 (the impact of TMB concentration), when the concentration of TMB is

[0040] When the concentration was 0.070 mol / L, the synthesis yield of PEI-CDs was the highest, the emission wavelength was the longest, and the PLQY was the largest.

[0041] Examples 10-14 illustrate that the concentration ratio of reactants is an important factor affecting the reaction of TMB and PEI. With the gradual increase of the concentration ratio of TMB and PEI, the synthesis yield and emission wavelength of PEI-CDs gradually decreased. However, the change of PLQY was in the form of a peak curve, and reached the maximum at the concentration ratio of TMB and PEI of 2:1, because with the increase of TMB dosage, the carbon source increased, and more CDs were absorbed by -NH 2 Modified to generate PEI-CD, the fluorescence performance of CDs is improved, so the PLQY gradually increases; however, when the concentration ratio of TMB and PEI is too large, the amount ...

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Abstract

The invention discloses a preparation method of PEI-CDs (polyetherimide-carbon dots) capable of emitting orange fluorescence. The preparation method comprises the steps that tetramethyl benzidine (TMB) and PEI are subjected to ultrasonic treatment until TMB and PEI are completely dissolved, then the mixture is transferred into a high-pressure reaction kettle and subjected to a reaction, and PEI modified CDs are synthesized by a hydrothermal method by one step; and after the PEI modified CDs are cooled to the room temperature, an obtained PEI-CDs stock solution is dialyzed by a dialysis bag andsubjected to rotary evaporation and concentration, system polarity reduction and petroleum ether precipitation, PEI-CDs precipitates are obtained, washed and subjected to vacuum drying, and solid PEI-CDs are obtained. The synthesis technology is simple, the cost is low, the yield is high, and the post-treatment method is novel. The synthesized PEI-CDs have good water solubility, high stability and particle uniformity, emit orange fluorescence under excitation of a visible light source, have high cell safety and high cellular uptake capacity, and can effectively transmit SiRNA to cross biological membranes to enter cells.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a preparation method of PEI-modified orange fluorescent emission carbon dots. Background technique [0002] Carbon dots (CDs) are nanomaterials with a size less than 10 nm and fluorescent emission. CDs have been widely used in bioimaging, biosensing, tumor gene therapy, drug delivery, chemical probes, and photocatalysis due to their good water solubility, high stability, low toxicity, easy functionalization, excellent optical properties, and simple preparation methods. and other fields (S. Y. Lim, W. Shen, et al. Chem. Soc. Rev. 2015 (44): 362-381). [0003] Since Sun et al. reported the preparation of carbon nanodots by physical cleavage and proposed the concept of CDs in 2006, the research on CDs has flourished, and researchers have developed many methods to optimize the performance of CDs. Practice has shown that element doping is an effective means to adjust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C09K11/02B82Y20/00B82Y30/00B82Y40/00
CPCC09K11/65C09K11/025B82Y20/00B82Y30/00B82Y40/00
Inventor 胡琴魏芳弟李瑞吴雪晴周萍岑瑶许贯虹陈秋彤程霞张爱霞
Owner NANJING MEDICAL UNIV
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