Preparation method for carboxyl and polypeptide modified AIE polymer nanoparticle

A nanoparticle and polypeptide modification technology, applied in chemical instruments and methods, luminescent materials, etc., can solve problems such as limiting the application value in the field of selective cell imaging

Active Publication Date: 2019-08-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not bee

Method used

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  • Preparation method for carboxyl and polypeptide modified AIE polymer nanoparticle
  • Preparation method for carboxyl and polypeptide modified AIE polymer nanoparticle
  • Preparation method for carboxyl and polypeptide modified AIE polymer nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Weigh 0.2g O-50 emulsifier and dissolve it in 12.5g deionized water to obtain an aqueous phase solution.

[0082] Weigh 0.01g of AIE-6 molecules and 0.02g of azobisisobutyronitrile and dissolve in a mixed solution of 0.06g of n-hexadecane, 0.1g of acrylic acid, 0.45g of styrene and 0.45g of methyl methacrylate to obtain an oil phase solution Add the water phase solution to the oil phase solution, and make a coarse emulsion after stirring and pre-emulsifying; place the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 400W for 9 minutes to obtain a stable monomer Miniemulsion: Nitrogen was passed to remove oxygen, the temperature was raised to 65° C., and reacted for 6 hours under the protection of nitrogen to prepare a carboxyl-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h...

Embodiment 2

[0096] Weigh 0.5 g of Tween 20 and dissolve it in 15 g of deionized water to obtain an aqueous phase solution.

[0097] Weighing 0.0075g of AIE-12 molecules and 0.015g of azobisisoheptanonitrile were dissolved in a mixed solution of 0.08g of n-hexadecane, 0.15g of methacrylic acid and 1.35g of methyl methacrylate to obtain an oil phase solution; Add the water phase solution to the oil phase solution and pre-emulsify with stirring to obtain a coarse emulsion; place the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 200W for 20 minutes to obtain a stable monomer fine emulsion. Emulsion: Nitrogen was passed to remove oxygen, the temperature was raised to 60° C., and under the protection of nitrogen, the reaction was carried out for 12 hours to prepare a carboxyl-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change fr...

Embodiment 3

[0103] Weigh 0.3g of MOA-9 and dissolve in 15g of deionized water to obtain an aqueous phase solution.

[0104]Weigh 0.003g of AIE-15 molecules and 0.05g of azobisisobutyronitrile and dissolve in a mixed solution of 0.08g of n-hexadecane, 0.05g of methacrylic acid, 0.45g of methyl methacrylate and 0.55g of styrene to obtain oil phase solution; add the water phase solution to the oil phase solution, and make a coarse emulsion after stirring and pre-emulsifying; place the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 600W for 5 minutes to obtain a stable Monomer miniemulsion; pass nitrogen to remove oxygen, raise the temperature to 60°C, and react for 24 hours under the protection of nitrogen to prepare carboxyl-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, ev...

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Abstract

The invention provides a preparation method for a carboxyl and polypeptide modified AIE polymer nanoparticle. The preparation method comprises the following steps: 1) preparing an emulsifier aqueous solution; 2) dissolving an AIE molecule, an Ostwald ripening effect inhibitor and an oil-soluble initiator into a mixed solution of a carboxyl functional monomer and a hydrophobic monomer so as to obtain an oil-phase solution; 3) adding the emulsifier aqueous solution into the oil-phase solution, carrying out pre-emulsification under stirring so as to obtain a crude emulsion, carrying out ultrasonic treatment so as to prepare a monomer fine emulsion, and introducing nitrogen to remove oxygen so as to prepare a carboxyl modified AIE polymer nanoparticle emulsion; 4) dissolving a carbodiimide condensing agent into an acidic pH buffer solution, adding an obtained solution into the emulsion prepared in the step 3), and carrying out an activation reaction; 5) dissolving omega-aminomaleimide intoan alkaline pH buffer solution; 6) adding an omega-aminomaleimide solution into an emulsion prepared in the step 4), and carrying out a reaction; and 7) adding a polypeptide aqueous solution with theterminal containing a cysteine sequence unit into an emulsion prepared in the step 6), and carrying out a reaction so as to prepare the carboxyl and polypeptide modified AIE polymer nanoparticle.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing surface-modified aggregation-induced emission (AIE) polymer nanoparticle. [0003] (2) Background technology [0004] Fluorescent nanoparticles are an important class of functional nanomaterials, which have the characteristics of good water dispersibility, high photostability, low cytotoxicity, and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis, and biosensing. application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application to some extent. In 2001, Academician Tang Benzhong's research group first reported a new type of fluorescent molecule with AIE effect, which emits strong fluorescence due to the restricted rotation within ...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F220/14C08F220/06C08F236/22C09K11/06
CPCC08F212/08C08F220/14C09K11/06C09K2211/14C08F220/06C08F236/22
Inventor 曹志海梁小琴齐琪罗成林姚俊余棒方星宇胡亚新秘一芳
Owner ZHEJIANG SCI-TECH UNIV
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