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A kind of preparation method of the AIE polymer nanoparticle of amino group and polypeptide modification

A nanoparticle, polypeptide modification technology, applied in chemical instruments and methods, luminescent materials, etc., can solve problems such as restricting applications, and achieve the effect of promoting the uptake rate

Active Publication Date: 2021-07-16
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not been modified with specific peptides, which limits its application in the field of selective cell imaging to a certain extent.

Method used

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  • A kind of preparation method of the AIE polymer nanoparticle of amino group and polypeptide modification
  • A kind of preparation method of the AIE polymer nanoparticle of amino group and polypeptide modification
  • A kind of preparation method of the AIE polymer nanoparticle of amino group and polypeptide modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Weigh 0.2g of O-50 emulsifier and 0.1g of 2-aminoethyl methacrylate hydrochloride, and dissolve them in 12.5g of deionized water to obtain an aqueous phase solution.

[0090] Weigh 0.01g of AIE-6 molecule and dissolve it in a mixed solution of 0.06g of n-hexadecane and 0.9g of methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase solution and pre-emulsify by stirring Finally, a coarse emulsion was obtained; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 400W for 9 minutes; Amidine hydrochloride, the temperature was raised to 65° C., and reacted for 6 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, ...

Embodiment 2

[0107] Weigh 0.5 g of Tween 20 and 0.15 g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15 g of deionized water, and obtain an aqueous phase solution.

[0108] Weigh 0.0075g of AIE-12 molecule and dissolve it in the mixed solution of 0.08g of n-hexadecane and 1.35g of styrene to obtain an oil phase solution; add the water phase solution to the oil phase solution and pre-emulsify it with stirring to obtain Coarse emulsion; place the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 200W for 20 minutes to obtain a stable monomer fine emulsion; pass nitrogen to remove oxygen, add 0.015g of azobisisobutylamidine hydrochloride salt, the temperature was raised to 60° C., and reacted for 24 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1The dialysis bag was dialyzed for three days, and the water change frequency...

Embodiment 3

[0114] Weigh 0.3g of MOA-9 and 0.05g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15g of deionized water, and obtain an aqueous phase solution.

[0115] Weigh 0.05g of AIE-15 molecules and dissolve in a mixed solution of 0.08g n-hexadecane, 0.45g styrene and 0.5g methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase solution , after stirring and pre-emulsifying, a coarse emulsion was obtained; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 600W for 5 minutes; nitrogen and deoxygenation, adding 0.05g Azobisisobutylamidine hydrochloride, the temperature was raised to 65° C., and reacted for 3 hours under the protection of nitrogen to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change...

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Abstract

A method for preparing amino and polypeptide modified AIE polymer nanoparticles, comprising: 1) dissolving an emulsifier and an amino functional monomer in water to obtain an aqueous phase solution; 2) dissolving AIE molecules and Oswald aging effect inhibitors in Obtain an oil phase solution from the hydrophobic monomer; 3) Add the water phase solution to the oil phase solution and stir and pre-emulsify to obtain a coarse emulsion, and ultrasonically treat it to obtain a monomer fine emulsion; pass nitrogen to remove oxygen, and add a water-soluble initiator to react. Amino-modified AIE polymer nanoparticle emulsion; 4) ω-maleimide alkanoic acid and carbodiimide condensing agent are dissolved in an acidic pH buffer and activated to prepare an activated intermediate solution; 5) the activated intermediate Add the body solution to the emulsion prepared in step 3) to react to prepare the maleimide-modified AIE polymer nanoparticle emulsion; 6) add the aqueous solution of the polypeptide containing cysteine ​​sequence unit at the end to the prepared in step 5) Amino- and peptide-modified AIE polymer nanoparticles were prepared by reacting in emulsion.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing surface-modified aggregation-induced emission (AIE) polymer nanoparticle. [0003] (2) Background technology [0004] Fluorescent nanoparticles are an important class of functional nanomaterials, which have the characteristics of good water dispersibility, high photostability, low cytotoxicity, and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis, and biosensing. application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application value to a certain extent. In 2001, Academician Tang Benzhong's research group first reported a new type of fluorescent molecule with AIE effect, which emits strong fluorescence due to the restricted rotat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/14C08F220/34C08F212/08C08F220/18C08F220/60C08F2/44C08F2/30C08K5/315C08K5/5399C08K5/55C08K5/3415C08K5/3437C08K5/46C08F8/30C08F8/34C09K11/02C09K11/06
CPCC08F2/30C08F2/44C08F8/34C08F212/08C08F220/14C08K5/315C08K5/3415C08K5/3437C08K5/46C08K5/5399C08K5/55C09K11/02C09K11/06C08F220/34C08F8/30C08F220/1804C08F220/60
Inventor 曹志海胡亚新齐琪王思彦温礼馨裘乘锦梁小琴秘一芳
Owner ZHEJIANG SCI-TECH UNIV
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