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A kind of preparation method of the AIE polymer nanoparticle of carboxyl group and polypeptide modification

A nanoparticle and polypeptide modification technology, which is applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of limiting the application value in the field of selective cell imaging, and achieve strong cell uptake ability, good biocompatibility, and cell imaging effect Good results

Active Publication Date: 2021-07-20
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not been modified with specific peptides, which limits its application value in the field of selective cell imaging

Method used

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  • A kind of preparation method of the AIE polymer nanoparticle of carboxyl group and polypeptide modification
  • A kind of preparation method of the AIE polymer nanoparticle of carboxyl group and polypeptide modification
  • A kind of preparation method of the AIE polymer nanoparticle of carboxyl group and polypeptide modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Weigh 0.2g O-50 emulsifier and dissolve it in 12.5g deionized water to obtain an aqueous phase solution.

[0085] The AIE-6 molecule that takes 0.01g is dissolved in the mixed solution of 0.06g n-hexadecane, 0.1g acrylic acid, 0.45g styrene and 0.45g methyl methacrylate, obtains oil phase solution; Add water phase solution to In the oil phase solution, the coarse emulsion was obtained after stirring and pre-emulsification; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 400W for 9 minutes; , add 0.02g of potassium persulfate, the temperature rises to 70°C, and react for 6h under the protection of nitrogen to prepare carboxy-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, every 3h on the second day, an...

Embodiment 2

[0099] Weigh 0.5 g of Tween 20 and dissolve it in 15 g of deionized water to obtain an aqueous phase solution.

[0100] Weigh 0.0075g of AIE-12 molecules and dissolve in a mixed solution of 0.08g n-hexadecane, 0.15g methacrylic acid and 1.35g methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase solution In the medium, the coarse emulsion was obtained after stirring and pre-emulsification; the container containing the coarse emulsion was placed in an ice-water bath, and the ultrasonic wave with a power of 200W was used for 20 minutes to obtain a stable monomer fine emulsion; g ammonium persulfate, the temperature was raised to 70° C., and reacted for 10 h under nitrogen protection to prepare a carboxyl-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, every 3h on the second da...

Embodiment 3

[0106] Weigh 0.3g of MOA-9 and dissolve in 15g of deionized water to obtain an aqueous phase solution.

[0107] The AIE-15 molecule that weighs 0.003g is dissolved in the mixed solution of 0.08g n-hexadecane, 0.05g methacrylic acid, 0.45g methyl methacrylate and 0.5g styrene to obtain an oil phase solution; Add it to the oil phase solution, and make a coarse emulsion after stirring and pre-emulsifying; put the container containing the coarse emulsion in an ice-water bath, and use an ultrasonic wave with a power of 600W for 5 minutes to prepare a stable monomer fine emulsion; Deoxygenation was added, and 0.05 g of potassium persulfate was added, and the temperature was raised to 72° C., and reacted for 6 h under the protection of nitrogen to prepare a carboxyl-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h on the first day, every 3h on ...

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Abstract

A method for preparing carboxyl- and polypeptide-modified AIE polymer nanoparticles, comprising: 1) preparing an aqueous emulsifier solution; 2) dissolving AIE molecules and Oswald aging effect inhibitors in a mixed solution of carboxyl functional monomers and hydrophobic monomers 3) add the emulsifier aqueous solution to the oil phase solution and stir to pre-emulsify to obtain a coarse emulsion, and ultrasonically treat it to obtain a monomer fine emulsion; pass nitrogen to remove oxygen, add a water-soluble initiator to react; 4) put The carbodiimide condensing agent is dissolved in an acidic pH buffer, and then added to the emulsion prepared in step 3) to perform an activation reaction; 5) ω-aminomaleimide is dissolved in an alkaline pH buffer to obtain ω-aminomaleimide solution; 6) adding ω-aminomaleimide solution to the emulsion prepared in step 4) for reaction; 7) adding the aqueous polypeptide solution containing cysteine ​​sequence units at the end to To react in the emulsion prepared in step 6) to prepare carboxyl- and polypeptide-modified AIE polymer nanoparticles.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing surface-modified aggregation-induced emission (AIE) polymer nanoparticle. [0003] (2) Background technology [0004] Fluorescent nanoparticles are an important class of functional nanomaterials, which have the characteristics of good water dispersibility, high photostability, low cytotoxicity, and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis, and biosensing. application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application to some extent. In 2001, Academician Tang Benzhong's research group first reported a new type of fluorescent molecule with AIE effect, which emits strong fluorescence due to the restricted rotation within ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C08F220/14C08F220/06C08F220/18C08F222/02C08F8/34C08F8/32C08K5/315C08K5/5399C08K5/55C08K5/3415C08K5/3437C08K5/46C09K11/06
CPCC08F8/34C08F212/08C08F220/14C08K5/315C08K5/3415C08K5/3437C08K5/46C08K5/5399C08K5/55C09K11/06C09K2211/14C09K2211/1425C08F220/06C08F8/32C08F220/1804C08F222/02
Inventor 曹志海梁小琴齐琪余棒方星宇裘乘锦胡亚新秘一芳
Owner ZHEJIANG SCI-TECH UNIV
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