Preparation method and application of nanoparticle of ribonucleoprotein compound capable of achieving tumor targeted delivery
A ribonucleoprotein and tumor-targeting technology, which is applied in the field of nanoparticle preparation, achieves the effects of simple preparation method, easy popularization and application, and easy operation
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Embodiment 1
[0027] Example 1: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.
[0028] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:
[0029] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)
[0030] First add cis-aconitic anhydride 0.276g (1.5mmol) in the round botto...
Embodiment 2
[0040] Example 2: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.
[0041] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:
[0042] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)
[0043] First, add cis-aconitic anhydride 0.276g (1.5mmol) to the round bott...
Embodiment 3
[0053] Example 3: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.
[0054] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:
[0055] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)
[0056] First, add cis-aconitic anhydride 0.276g (1.5mmol) in the round bott...
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