Preparation method and application of nanoparticle of ribonucleoprotein compound capable of achieving tumor targeted delivery

A ribonucleoprotein and tumor-targeting technology, which is applied in the field of nanoparticle preparation, achieves the effects of simple preparation method, easy popularization and application, and easy operation

Inactive Publication Date: 2020-01-03
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of effective delivery vectors to efficiently deliver Cas9 protein and sgRNA to tumor tissues, and the delivery vector also needs to protect Cas9 protein and sgRNA from being degraded by related enzymes in serum

Method used

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  • Preparation method and application of nanoparticle of ribonucleoprotein compound capable of achieving tumor targeted delivery
  • Preparation method and application of nanoparticle of ribonucleoprotein compound capable of achieving tumor targeted delivery
  • Preparation method and application of nanoparticle of ribonucleoprotein compound capable of achieving tumor targeted delivery

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.

[0028] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:

[0029] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)

[0030] First add cis-aconitic anhydride 0.276g (1.5mmol) in the round botto...

Embodiment 2

[0040] Example 2: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.

[0041] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:

[0042] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)

[0043] First, add cis-aconitic anhydride 0.276g (1.5mmol) to the round bott...

Embodiment 3

[0053] Example 3: A method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes.

[0054] attached figure 1 The schematic diagram of the preparation and application of the nano-ribonucleoprotein in the present invention is shown, and the nano-ribonucleoprotein prepared by the method of the present invention is a uniformly distributed spherical particle with a particle diameter of 120±15 nm. The nanoparticle firstly consists of a negatively charged ribonucleoprotein complex formed by Cas9 protein and sgRNA, and then forms a nano ribonucleoprotein complex with an environment-responsive polymer through electrostatic self-assembly. See attached figure 1 , a method for preparing nanoparticles capable of tumor-targeted delivery of ribonucleoprotein complexes, comprising the steps of:

[0055] 1) Using cis-aconitic anhydride to modify polyethylene glycol (mPEG-CA)

[0056] First, add cis-aconitic anhydride 0.276g (1.5mmol) in the round bott...

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Abstract

The invention discloses a preparation method and application of nanoparticles of a ribonucleoprotein compound capable of achieving tumor targeted delivery. The nanoparticles are formed by self-assembly of a polymer with tumor microenvironment responsiveness and the ribonucleoprotein compound, the polymer with tumor microenvironment responsiveness allows the nanoparticles to maintain stability in the blood circulation process, and meanwhile the enrichment of the nanoparticles in tumor tissue is enhanced. The nanoparticles of the ribonucleoprotein compound carrying fluorescent probe marks are injected to mice with tumor, the effective enrichment of the ribonucleoprotein compound in the tumor tissue is achieved, and it is indicated that the delivery of the ribonucleoprotein compound with tumor targeted delivery can be completely achieved by the nanoparticles.

Description

technical field [0001] The invention belongs to the field of macromolecule biomaterials, and relates to a preparation method and application of nanoparticles capable of realizing tumor-targeted delivery of ribonucleoprotein complexes. Background technique [0002] Tumor heterogeneity observed in most malignancies refers to differences in phenotypic characteristics (i.e., cell morphology, gene expression, metabolism, proliferation, angiogenesis, and metastatic potential) among individual cells in a patient's tumor. As tumors grow, this heterogeneity leads to the accumulation of genetically distinct tumor cell subpopulations, which presents a major challenge to the design of tumor treatment strategies. Although conventional treatment strategies (such as chemotherapy, immunotherapy, and gene therapy) may achieve initial success, the existence of a small number of drug-resistant cell subsets will lead to tumor recurrence, resulting in the failure of tumor treatment. [0003] To...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K48/00A61P35/00
CPCA61K9/5146A61K48/005A61P35/00
Inventor 刘阳刘琦康春生
Owner NANKAI UNIV
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