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Targeted nano-vaccine preparation based on metal-polyphenol network structure and product of targeted nano-vaccine preparation

A network structure and nano-vaccine technology, applied in the field of targeted nano-vaccine preparation and its products, nano-vaccine preparation and its products, can solve the weak response of antigen-specific cytotoxic T lymphocytes and increase the escape function of nano-particle lysosomes , affecting biocompatibility and in vivo safety and other issues, to achieve the effects of enhancing cellular uptake, improving targeting and lysosomal escape efficiency, and improving vaccine delivery efficiency

Inactive Publication Date: 2021-01-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still many problems to be solved in the construction of nano-vaccine: for example, the use of a large number of organic reagents seriously affects its biocompatibility and in vivo safety; the targeting of the vaccine is weak; the lysosome escape efficiency of the vaccine Low, weak induction of antigen-specific cytotoxic T lymphocyte (CTL) response, etc.
[0005] After retrieval, the use of mesoporous silica nanoparticles with good biocompatibility, electrostatic adsorption mode antigen ovalbumin (OVA), through the surface coating metal-polyphenol network (Metal-Phenolic Network, MPN) coating to prevent The leakage of OVA increases the lysosome escape function of nanoparticles; at the same time, it modifies mannose on the surface, targets the mannose receptor on the surface of immune cells, and enhances the uptake ability of cells to improve the efficiency of vaccine delivery based on the metal-polyphenol network The targeted nano-vaccine of the structure and its preparation method have not been reported yet

Method used

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  • Targeted nano-vaccine preparation based on metal-polyphenol network structure and product of targeted nano-vaccine preparation
  • Targeted nano-vaccine preparation based on metal-polyphenol network structure and product of targeted nano-vaccine preparation
  • Targeted nano-vaccine preparation based on metal-polyphenol network structure and product of targeted nano-vaccine preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Synthesis of mesoporous silica nanoparticles (optimization of conventional preparation methods in literature)

[0044] According to the synthesis method disclosed in the literature, mesoporous silica nanoparticles with a preset particle size of about 110 nm were synthesized.

[0045] First, weigh 960 mg of cetyltrimethyl-p-toluenesulfonium ammonium (CTAT) into a round bottom flask, and add 50 mL of deionized water to dissolve it. At the same time, 174 mg of triethanolamine (TEA) was added to dissolve and mix well. The temperature of the oil bath was kept at 80° C. and stirred for 1 h, 7.81 mL of tetraethyl orthosilicate (TEOS) was slowly added dropwise, and the stirring was continued for two hours. The obtained product was washed three times with water and three times with ethanol (centrifugation condition: 13000g, 10min). Then the product was dried in an oven at 60°C for 12 hours. Grind it with a mortar and keep it in a muffle furnace for 6 hours at 600°C...

Embodiment 2

[0046] Example 2: Synthesis of mannose-modified tannic acid molecules

[0047] Weigh 3.25mg of aminated mannose (purchased from Sigma) and 20mg of tannic acid, then dissolve the two compounds in 5mL of MOPS buffer solution with pH=8.5, stir magnetically for 12h, and use a dialysis bag with a molecular weight of 700K Dialysis to obtain mannose-modified tannic acid molecules.

Embodiment 3

[0048] Example 3: Amination of Mesoporous Silica Nanoparticles

[0049] Accurately weigh 30 mg of mesoporous silica nanoparticles, dissolve them in 900 μL of ethanol solution, and ultrasonicate for 2 min to uniformly disperse the mesoporous silica nanoparticles in the ethanol solution, then slowly add 50 μL of ammonia water. Add 30 μL of 3-aminopropyltriethoxysilane (APTES) under the condition of vortexing, vortex for 30 s after the addition, and place in a closed reaction vial for overnight magnetic stirring.

[0050] The solution after the overnight reaction was centrifuged at 8000r / min for 5min, the precipitated particles were washed 3 times with methanol, and then washed 3 times with water to obtain aminated mesoporous silica nanoparticles, which were stored at 4°C for characterization and subsequent experiments.

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Abstract

The invention discloses a preparation method of a targeted nano vaccine based on a metal-polyphenol network structure. The preparation method comprises the following steps that mesoporous silica nanoparticles loaded with ovalbumin OVA are prepared, mannose-modified tannic acid molecules are synthesized, and the surfaces of the mesoporous silica nanoparticles loaded with the ovalbumin OVA are coated with metal-polyphenol network coatings, so that the mesoporous silica nanoparticles loaded with the OVA and with the surfaces coated with the metal-polyphenol network coatings are obtained, therefore the targeted nano-vaccine based on the metal-polyphenol network structures is obtained, and the targeted nano-vaccine is named as MS@OVA@MPN@Man. According to the preparation method, the surfaces are coated with the metal-polyphenol network coatings, so that leakage of OVA is prevented, and the lysosome escape function of the nanoparticles is increased; and meanwhile, mannose is modified on thesurface to target a mannose receptor on the surface of an immune cell, so that the uptake capacity of the cell to the mannose receptor is enhanced to improve the vaccine delivery efficiency, and the problems of targeting of the nano vaccine and lysosome escape efficiency are solved.

Description

technical field [0001] The invention relates to a preparation of a nano-vaccine and its product, in particular to a preparation of a targeted nano-vaccine based on a metal-polyphenol network structure and its product, and belongs to the technical field of nano-materials and biomedicine. Background technique [0002] Nanotechnology is the commanding height of scientific and technological development in the 21st century and the leading technology of the new industrial revolution. Due to their unique structural, magnetic, mechanical and size effects, nanoparticles are widely used in fields including biosensing, drug delivery, bioimaging, catalysis, nanofabrication, lubrication, electronics, textiles, etc. One of the current research hotspots is to study and explore various nanoparticle systems used in the biomedical field, especially nanoparticles used in tumor therapy, including mesoporous silica, metal nanoparticles, magnetic oxides, and quantum dots. and other inorganic nan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/04A61K47/26A61K39/00A61P35/00
CPCA61K9/5115A61K9/5123A61K39/0011A61P35/00
Inventor 翟淑梅杨勋成崔基炜张贵强张培育孙海峰
Owner SHANDONG UNIV
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