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Enhanced photodynamic anti-tumor nano-drug loading system and preparation method and application thereof

A nano-drug-loading and photodynamic technology, applied in the field of anti-tumor drugs, can solve problems such as poor treatment effect, and achieve the effect of enhancing anti-tumor effect, high biosafety, and good biocompatibility

Active Publication Date: 2019-07-26
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides an enhanced photodynamic anti-tumor nano drug-carrying system and its preparation method and application in order to overcome the defect that the existing photodynamic therapy method has poor therapeutic effect due to the tumor tissue and the surrounding hypoxic environment.

Method used

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  • Enhanced photodynamic anti-tumor nano-drug loading system and preparation method and application thereof
  • Enhanced photodynamic anti-tumor nano-drug loading system and preparation method and application thereof
  • Enhanced photodynamic anti-tumor nano-drug loading system and preparation method and application thereof

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preparation example Construction

[0036] The present invention also provides a method for preparing the nano drug-carrying system described in the above technical solution, comprising the following steps:

[0037] (1) dissolving the nano-lipid material and the chemotherapeutic drug in an organic solvent, adding dropwise to the solution containing the near-infrared photosensitizer, and stirring to obtain nanoliposomes coated with the chemotherapeutic drug and the near-infrared photosensitizer;

[0038] (2) modifying a layer of branched gold nanoshells on the surface of the nanoliposomes obtained in step (1) by the seed crystal growth method to obtain nanoliposomes modified by branched gold nanoshells;

[0039] (3) The nano liposome obtained in the step (2) is mixed with catalase, incubated, and the catalase is modified on the surface of the branched gold nano spherical shell to obtain an enhanced photodynamic anti-tumor nano-drug loading system.

[0040] The invention dissolves the nano lipid material and the c...

Embodiment 1

[0053] Dissolve 3 mg of cholesterol (purchased at Tianjin Damao Chemical Instrument Supply Station), 30 mg of soybean lecithin (purchased at Shenyang Tianfeng Biological Co., Ltd.) and 1 mg of paclitaxel (purchased at Shanghai Yuanye Biological Co., Ltd.) in 3 mL of absolute ethanol , sonicate to dissolve it, and drop it dropwise into 8 mL of indocyanine green solution with a concentration of 0.5 mg / mL, and magnetically stir for 1 hour to obtain paclitaxel indocyanine green nanoliposomes. Add 5 μL of 200 mM sodium borohydride solution to 1.0 mL of 0.5 mM gold chloride solution and shake vigorously to prepare gold nanoparticle solution; mix each 400 μL of gold nanoparticle solution with paclitaxel indocyanine green nanoliposomes , add 300 μL of 1.5 mM gold chloride solution and 30 μL of 100 mM hydroxylamine hydrochloride, and incubate for 8 hours to obtain paclitaxel indocyanine green nanoliposomes modified with branched gold spherical shells; 75 μg / ml catalase The solution was...

Embodiment 2

[0056] Dissolve 4mg of cholesterol (purchased at Tianjin Damao Chemical Instrument Supply Station), 40mg of soybean lecithin (purchased at Shenyang Tianfeng Biological Co., Ltd.) and 3mg of paclitaxel (purchased at Shanghai Yuanye Biological Co., Ltd.) in 5mL of absolute ethanol , sonicate to dissolve it, and drop it dropwise into 9mL of 0.75mg / mL indocyanine green solution, and magnetically stir for 1.5 hours to obtain paclitaxel indocyanine green nanoliposomes; in 1.5mL of 0.75mM Add 7.5 μL of 225 mM sodium borohydride solution to the gold chloride solution and shake vigorously to prepare the gold nanoparticle solution; mix each 450 μL of the gold nanoparticle solution with paclitaxel indocyanine green nanoliposomes, add 400 μL of 1.75 mM Gold chloride solution and 40 μL of 150 mM hydroxylamine hydrochloride were incubated for 9 hours to obtain paclitaxel indocyanine green nanoliposomes modified with branched gold spherical shells; catalase was added to the prepared branched ...

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Abstract

The invention provides an enhanced photodynamic anti-tumor nano-drug loading system which relates to the technical field of anti-tumor drugs. The nano-drug loading system is modified with hydrogen oxide enzyme, dendritic gold nanosphere shells and nano-liposomes sequentially from inside to outside, and the nano-liposomes are coated with chemotherapeutic drugs and near-infrared photosensitizers. The nano-drug loading system modified with hydrogen oxide enzyme used for increasing the oxygen content around the tumor tissue and overcoming the limitation of photodynamic treatment effect of the tumor tissue and surrounding hypoxia of the same; on the other hand, the problems about limitations of a single treatment mode and poor anti-tumor effect are solved; the combination of chemotherapy and photodynamic therapy is adopted, mutual promotion under various treatment modes can eliminate tumor cells more efficiently and deeply, and the anti-tumor effect is improved.

Description

technical field [0001] The invention relates to the technical field of anti-tumor drugs, in particular to an enhanced photodynamic anti-tumor nano-drug loading system and its preparation method and application. Background technique [0002] At present, traditional tumor chemotherapy has problems such as poor targeting, high toxicity and side effects, single treatment mechanism, and poor treatment effect. In the tumor-specific microenvironment (high hydrogen peroxide, weak acidity, hypoxia, etc.), nano-drug carriers can convert spatiotemporal controllable energy such as light, ultrasound, and magnetic field into therapeutic factors for tumors, resulting in a variety of biological effects. [0003] Photodynamic therapy is a new technology for disease diagnosis and treatment using photodynamic effect. It is a photosensitization reaction accompanied by biological effects involving oxygen molecules. Excited, and the photosensitizer in the excited state transfers energy to the su...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/42A61K47/02A61K41/00A61K31/337A61P35/00
CPCA61K9/1271A61K31/337A61K41/0057A61K47/02A61K47/42A61P35/00A61K2300/00
Inventor 高大威何雨初丛聪李磊骆丽垚
Owner YANSHAN UNIV
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