Methods of using microbial cleavage reactions to reduce abundance of multiple antibiotic resistance genes in excess sludge and limit their level transfer

Abstract

The invention relates to a method for reducing abundance of multiple antibiotic resistance genes in excess sludge and limiting horizontal transfer of the multiple antibiotic resistance genes. According to the method, parasitic bdellovibrio bacteriovorus can invade other host cells, and polypeptide, amino acid and the like in the host cells are used as energy and a carbon source for self-proliferation; the genetic material in the sludge host cell carrying the antibiotic resistance gene is degraded and reconstructed so as to achieve the purpose of reducing the abundance of a variety of resistance genes, and the degradation of the movable gene element (integron I) further effectively inhibits the horizontal transfer of the antibiotic resistance gene during the biotransformation process. According to the method disclosed by the invention, various resistance genes in the residual sludge can be remarkably reduced, which comprises the abundance of sulfonamides (sul1, sul2), tetracyclines (tetC, tetQ) and class I integron (int1); meanwhile, equipment is simple, energy is saved, secondary pollution is avoided, and an environment-friendly, green, low-carbon, economical and efficient ecological way is provided for relieving antibiotic resistance gene pollution of municipal excess sludge, developing series sludge recycling products such as landscaping matrix soil and soil conditioners, widening the land utilization range and widening the utilization amount of the recycling products.

Description

technical field [0001] The invention relates to a method for reducing the abundance of multiple antibiotic resistance genes in excess sludge and limiting their horizontal transfer by using microbial cracking reaction, which belongs to the technical field of environmental protection. Background technique [0002] As a broad-spectrum antibacterial drug, antibiotics are widely used and used in huge amounts, and the production and consumption of antibiotics in my country has been ranking first in the world. Antibiotics present in the environment can induce drug resistance in bacteria under stress, and resistance genes are the basis for the formation and spread of bacterial drug resistance, which can be acquired by gene mutation or horizontal transfer. Resistance genes do not depend on their biological toxicity, and are hereditary and self-replicating. They can be transferred and spread among species by means of combination, transduction and transformation through mobile genetic ...

AI Technical Summary

Problems solved by technology

[0006] In view of the above existing problems, the purpose of the present invention is to provide a method for degrading and reconstructing genetic material in sludge host cells carrying antibiotic resistance genes by using parasitic Bdellovibrio, thereby effectively reducing various The abundance of resistance genes (including sulfonamides (sul1, sul2), tetracyclines (tetC, tetQ)) and the suppression of multiple Antibiotic resistance gene spreads horizontally without causing secondary pollution to the environment. The green and environmentally friendly removal process of resistance genes can alleviate the problem of antibiotic resistance gene pollution and provide support for the protection of human public health

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