Method for preparing bdellovibrio preparation by enrichment culture method

A technology for phagocytic Bdellovibrio and enrichment culture, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of ineffective application and limited lysing ability of various pathogenic bacteria

Active Publication Date: 2018-12-28
福建九为生物技术有限公司
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Problems solved by technology

The above-mentioned Bdellovibrio preparations used in the production of Bdellovibrio preparations are all single Bdellovibrio strains obtained after purification as bacterial species, and there are usually multiple pathogenic bacteria in the aquaculture water body at the same time, and a single Bdellovibrio strain is effective against a variety of pathogenic bacteria. The cracking ability of germs is limited, so there are problems such as the application effect is not obvious

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  • Method for preparing bdellovibrio preparation by enrichment culture method

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Embodiment 1

[0028] A method for preparing a phage Vibrio preparation by an enrichment culture method, the specific steps are as follows:

[0029] Step 1: Isolation of host bacteria: Take 0.1 ml of water below 20 cm in the seawater shrimp pond in the mid-cultivation stage, inoculate it in TCBS Vibrio selective medium, spread evenly, and place it in a 35°C constant temperature incubator for upside-down culture 24 hours; the blue-green colonies that appear are Vibrio parahaemolyticus; pick a single colony of Vibrio parahaemolyticus and inoculate it on a TCBS plate for 3 to 5 consecutive times, until the microscopic observation of the bacteria and the colony morphology are consistent. Colony, inoculated in NB test tube for 24 hours, is the host strain of Vibrio parahaemolyticus of this preparation;

[0030] The culturing conditions of the TCBS Vibrio selective medium are as follows: the control temperature is 35° C. and the culturing time is 24-36 hours.

[0031] Step 2: Preparation of the h...

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Abstract

The invention relates to a method for preparing a bdellovibrio preparation by an enrichment culture method, belongs to the field of biotechnology and relates to an enrichment culture method of variousbdellovibrio, host bacteria culture and production methods of biological preparations thereof. The method is characterized in that a bdellovibrio system naturally formed in aquaculture water culturedby the enrichment culture method is used as a source of the bdellovibrio of the biological preparation, and pathogenic vibrio in the aquaculture water is purified, cultured, centrifuged, inactivatedand then used as a source of the host bacteria for the production of the bdellovibrio preparation to produce the biological preparation containing various bdellovibrio. The bdellovibrio preparation produced by the method has the advantages of high activity, rapid action and broad cleavage of pathogenic bacteria in the water due to the various bdellovibrio.

Description

technical field [0001] The invention belongs to the technical field of microbial sterilization, and in particular relates to a method for preparing a leech vibrio preparation by an enrichment culture method. Background technique [0002] At present, the method of eliminating pathogenic bacteria in aquaculture water mainly uses various disinfectants and antibiotics to disinfect and purify the water body. This method can quickly kill pathogenic bacteria in the water body, but also kill the beneficial microorganisms in the water body. Destroy the micro-ecosystem of the water body. The long-term use of disinfectants and antibiotics leads to the increase of drug resistance of pathogenic bacteria year by year, and the use of disinfectants and antibiotics cannot effectively control pathogenic bacteria. [0003] In recent years, the use of beneficial microorganisms to control pathogenic microorganisms in aquaculture has attracted more and more attention, especially the leech vibrio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/63
CPCC12N1/20
Inventor 文传敬谢荔朋
Owner 福建九为生物技术有限公司
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