Method for producing microecological preparation of bdellovibrio bacteriourus

A technology of microecological preparations and production methods, applied in the field of preparation of microbial preparations, can solve the problems of damaging the environment, unfavorable large-scale production, and unfavorable large-scale use of Bdellovibrio microecological preparations, etc. The effect of high number and small dose of disease prevention and treatment

Active Publication Date: 2009-01-07
SHANDONG SINDER TECH
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  • Summary
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production and cultivation of Bdellovibrio adopts the culture method of living host bacteria. On the one hand, it is difficult to obtain the host bacteria, which is not conducive to large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a. Preparation of host bacteria: inoculate Escherichia coli (number of bacteria 1.3×10) with 2% inoculum 8 cfu / ml) in a fermenter containing 3% peptone, 1% yeast powder, 1% sodium chloride sterilized 120L medium, 200rpm, 0.05MPa, 2.0m 3 Cultivate under the condition of / h for 4 hours and start to continuously add 50% glucose solution (add 20ml every 15 minutes, add 1000ml altogether) after cultivating under the condition of 4 hours, make dissolved oxygen in the fermented liquid maintain on 70% by adjusting the number of rotations of the fermentor and the ventilation rate, by Add 1M hydrochloric acid and 5M sodium hydroxide solution to maintain the pH in the fermenter between 7.0 and 7.3, and ferment for 18 hours to obtain the host bacteria fermentation liquid; centrifuge the fermentation (8000rpm, 15min) liquid to obtain the host bacteria strain, wash with sterilized tap water The host bacteria slime was obtained 3 times, and freeze-dried in a freeze-drying machine at -...

Embodiment 2

[0030] a. Preparation of host bacterium: inoculate Escherichia coli with 3% inoculum (number of bacteria 1.2×10 8 cfu / ml) in a fermenter containing 2% peptone, 0.6% yeast powder, 0.8% sodium chloride sterilized 120L medium, 200rpm, 0.05MPa, 2.0m 3 After cultivating for 5 hours under the / h condition, start to continuously add 50% glucose solution (add 20ml every 15 minutes, add 1200ml altogether), and make dissolved oxygen in the fermented liquid maintain at 80% by adjusting the number of rotations of the fermenter and the ventilation rate. Add 1M hydrochloric acid and 5M sodium hydroxide solution to maintain the pH in the fermenter between 7.0 and 7.3, and obtain the host bacteria fermentation liquid after 20 hours of fermentation; centrifuge the fermentation (8000rpm, 15min) liquid to obtain the host bacteria, and sterilize tap water Wash 3 times to get the host bacteria slime, freeze-dry in a freeze-drying machine at -20~-15°C to obtain 506 g of freeze-dried host bacteria p...

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PUM

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Abstract

The invention discloses a production method for a bdellovibrio micro-ecological preparation. The production method includes the following steps: A, preparing freezing-drying host bacteria powder; B, preparing the culture medium of the bdellovibrio; C, inoculating the seeds of the bdellovibrio with 2 to 5 percent into a fermentation pot loaded with the culture medium of the bdellovibrio, ventilating and culturing for 20 to 24 hours under 30 to 33 DEG C, namely obtaining the bdellovibrio micro-ecological preparation. The production technique of the host bacteria of the bdellovibrio micro-ecological preparation adopts the freezeing-drying technique to ensure the preservation quality of the host bacteria and provides a stable reliable proliferation nutrition source for the growing and proliferation of the bdellovibrio.

Description

technical field [0001] The present invention relates to a kind of preparation method of microbial preparation, specifically, the present invention is a kind of production method of Bdellovibrio microecological preparation. Background technique [0002] Bdellovibrio is a group of parasitic bacteria that mainly prey on pathogenic bacteria such as Escherichia coli, Salmonella, and Vibrio parahaemolyticus. As an effective biological agent for biological control of diseases, Bdellovibrio is favored by people in the animal disease control and environmental improvement industries. At present, the production and cultivation of Bdellovibrio adopts the culture method of living host bacteria. On the one hand, it is difficult to obtain the host bacteria, which is not conducive to large-scale production. Thereby, existing technology is unfavorable for large-scale industrial production and safe use shortcoming of Bdellovibrio probiotics, this process makes above-mentioned problem well so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/63
Inventor 李国保乔彦良崔增学
Owner SHANDONG SINDER TECH
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