A new method for cultivating Cordyceps militaris

A cultivation method and technology of Cordyceps militaris, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of high price, rare price, etc., and achieve the effects of simple structure, convenient operation and broad market prospect.

Inactive Publication Date: 2018-04-13
山西省医药与生命科学研究院
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Problems solved by technology

But Cordyceps sinensis is only wild at presen...

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  • A new method for cultivating Cordyceps militaris
  • A new method for cultivating Cordyceps militaris

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Embodiment Construction

[0029] The present invention is achieved in this way, a new method for cultivating Cordyceps militaris. The current production strain is fresh wild Cordyceps sinensis collected from Jilin, Liaoning and other places. Through tissue culture, plate separation, screening and purification, a strain with strong adaptability, stable properties and high yield is finally obtained. The strain code: Y201005bcc . Regular subculture, rejuvenation and domestication ensure that the strain does not degenerate and has high yield.

[0030] As shown in Table 1, the formula of the liquid strain is as follows: silkworm chrysalis powder 35g; glucose 35g; cornmeal 50g; VB 1 2 pieces; KH 2 PO 4 5g; MgSO 4 1.7g; water 3500ml.

[0031] As shown in Table 2, the fermenter formula is as follows: Cordyceps fermenter culture medium 70L / tank; soybean powder 200g; cornmeal 1400g; sucrose 1400g; KH 2 PO 4 70g; MgSO 4 70g; silkworm chrysalis powder 1050g; add 7ml of defoamer and 7-10ml of salad oil...

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Abstract

The invention discloses a new method for cultivating Cordyceps militaris, comprising the following steps: connecting solid strains of test tubes into shake flasks and converting them into liquid strains, inserting strains of shake flasks into fermenters for strain propagation, and solid strains of Cordyceps militaris Cultivation box medium production, liquid inoculation, mycelium culture, color change, grass management, and harvesting. Shake flask culture liquid strain formula is as follows: silkworm chrysalis powder 35g; glucose 35g; cornmeal 50g; VB1 2 pieces; KH2PO4 5g; MgSO4 1.7g; water 3500ml; Soybean flour 200g; cornmeal 1400g; sucrose 1400g; KH2PO4 70g; MgSO4 70g; ; MgSO4 1g; VB1 1 piece; soybean meal powder 16.67g; sugar 10g; water 1000ml. The new cultivation method provided by the invention has the advantages of convenient operation, short cultivation period, low cultivation cost, high cultivation success rate and high yield, and the content of cordycepin in adult grass is also greatly improved.

Description

technical field [0001] The invention belongs to the field of cultivating Cordyceps militaris, in particular to a new method for cultivating Cordyceps militaris. Background technique [0002] Cordyceps sinensis is a compound of the subsealets and larvae corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of bat moths. It is a traditional precious nourishing Chinese medicinal material, which has various functions such as regulating immune system function, anti-tumor, and anti-fatigue. effect. But Cordyceps sinensis is currently only wild, and because it is extremely rare, it is expensive. [0003] But Cordyceps sinensis is not a unique "magic grass", and the content of medicinal ingredients in Cordyceps militaris is not inferior. Comparing Cordyceps militaris with wild Cordyceps sinensis, it is found that the content of the most essential cordycepin is 7 times higher, and there are only a lot more nutrients such as cordycepic acid, cordyceps polysacchar...

Claims

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Application Information

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IPC IPC(8): A01G18/00C05G3/00
Inventor 郭素萍杨槐俊李新冬张治家孙聪聪
Owner 山西省医药与生命科学研究院
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