High-density bdellovibrio swim body fermenting and culturing technique

A technology of fermentation culture and leech vibrio, applied in bacteria and other directions, can solve the problems of large amount of use, delayed action, strong lysis ability of beneficial bacteria, etc., and achieve the effect of improving the control effect.

Inactive Publication Date: 2008-05-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, a method for producing bacteriophage Bdellovibrio preparations is proposed in China No. 200610039346.8 patent application for invention. Although the application of the Bdellovibrio preparations produced by this method can solve the side effects caused by current methods such as antibiotics, the The density of the produced Bdellovibrio preparation is low, resul

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] High-density Bdellovibrio swimming body fermentation and cultivation technology, the steps and process conditions of this technology are as follows:

[0026] Step 1: Preparation of host bacteria suspension:

[0027] A strain of Escherichia coli / Pseudomonas aeruginosa isolated in our laboratory was inoculated in the nutrient broth medium as a host bacterium, and cultured in a shaker at 20°C for 14 hours to make it in the logarithmic growth phase. , centrifuged at 5000rpm for 35min, retained the precipitate and discarded the supernatant, and added 0.05% dilute nutrient broth DNB culture solution to the precipitate in a volume ratio to obtain a concentration of 10 18 ~10 26 CFU / mL of the host bacteria suspension, and then stored in a refrigerator at 2-4°C for later use;

[0028] Step 2: Preparation of Bdellovibrio Swimming Body Concentrate:

[0029] In the dilute nutrient broth DNB culture liquid, first add the host bacteria suspension prepared in step 1, so that the co...

Embodiment 2

[0033] High-density Bdellovibrio swimming body fermentation and cultivation technology, the steps and process conditions of this technology are as follows:

[0034] Step 1: Preparation of host bacteria suspension:

[0035] Inoculate a strain of Vibrio parahaemolyticus or Vibrio bathensis or Edwardsiella isolated in our laboratory into the nutrient broth medium, and place it in a shaker at 26°C for 18 hours to make it in the logarithmic growth phase , the culture solution was centrifuged at 10°C and 6000rpm for 20min, the precipitate was retained and the supernatant was discarded, and the volume ratio of 0.5% dilute nutrient broth DNB was added to the precipitate to obtain a concentration of 10 18 ~10 26 CFU / mL of the host bacteria suspension, and then stored in a refrigerator at 2-4°C for later use;

[0036] Step 2: Preparation of Bdellovibrio Swimming Body Concentrate:

[0037] In the dilute nutrient broth DNB culture liquid, first add the host bacteria suspension prepared...

Embodiment 3

[0041] High-density Bdellovibrio swimming body fermentation and cultivation technology, the steps and process conditions of this technology are as follows:

[0042] Step 1: Preparation of host bacteria suspension:

[0043] Inoculate a strain of Aeromonas or Salmonella or Pseudomonas isolated in our laboratory in the nutrient broth medium as a host bacterium, and culture it in a shaker at 32°C for 24 hours to make it in the logarithmic growth phase , the culture solution was centrifuged at 15°C and 8000rpm for 15min, the precipitate was retained and the supernatant was discarded, and the volume ratio of 2% dilute nutrient broth DNB was added to the precipitate to obtain a concentration of 10 18 ~10 26 CFU / mL of the host bacteria suspension, and then stored in a refrigerator at 2-4°C for later use;

[0044] Step 2: Preparation of Bdellovibrio Swimming Body Concentrate:

[0045] In the dilute nutrient broth DNB culture liquid, first add the host bacteria suspension prepared in...

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Abstract

The invention relates to fermentation culturing technology of high density Bdellovibrio telotroch, comprising the steps as following: firstly, culturing the host-germ shaking table and adding DNB liquid after centrifugating to obtain host-germ suspension; secondly; adding the host-germ suspension into the DNB liquid and then adding the Bdellovibrio to culture, at last adding DNB liquid after centrifugating to obtain Bdellovibrio concentrated solution; thirdly; adding sodium glutamate into DNB liquid and then adding the host-germ suspension, at last adding the Bdellovibrio concentrated solution to make the concentration 1 to 10<3>PFU/mL. The fermentation liquid of the Bdellovibrio with density of 10<8> to 10<14>PFU/mL can be obtained by culturing in a fermentation cylinder for four to six days and the concentrated solution of Bdellovibrio telotroch can be obtained through concentration by centrifuging. The invention has the advantages of low cost, high final concentration of Bdellovibrio and wide application range, and can be used not only in the control of water source pathogen and food pathogen, but also in fields of medical science, environmental pollution, agriculture, industry and military matters.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and specifically refers to a fermentation and cultivation technology of high-density Bdellovibrio swimming bodies that can be applied to reduce or even completely eliminate harmful bacteria, not only in the prevention and control of water-borne and food-borne pathogenic bacteria, but also It will have great application and development prospects in medicine, environmental pollution, agriculture, industry, military fields, etc. Background technique [0002] Although the elimination of pathogenic bacteria by means of biological control is a research hotspot in various fields such as industry, agriculture, and medicine in the world today, the main means of eliminating pathogenic bacteria are still various physical and / or chemical methods, including various antibiotics. usage of. These methods all have obvious drawbacks. First, the excessive abuse of antibiotics will lead to some side effects,...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 蔡俊鹏王泽秀
Owner SOUTH CHINA UNIV OF TECH
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