High-density bdellovibrio swim body fermenting and culturing technique

Abstract

The invention relates to fermentation culturing technology of high density Bdellovibrio telotroch, comprising the steps as following: firstly, culturing the host-germ shaking table and adding DNB liquid after centrifugating to obtain host-germ suspension; secondly; adding the host-germ suspension into the DNB liquid and then adding the Bdellovibrio to culture, at last adding DNB liquid after centrifugating to obtain Bdellovibrio concentrated solution; thirdly; adding sodium glutamate into DNB liquid and then adding the host-germ suspension, at last adding the Bdellovibrio concentrated solution to make the concentration 1 to 10<3>PFU / mL. The fermentation liquid of the Bdellovibrio with density of 10<8> to 10<14>PFU / mL can be obtained by culturing in a fermentation cylinder for four to six days and the concentrated solution of Bdellovibrio telotroch can be obtained through concentration by centrifuging. The invention has the advantages of low cost, high final concentration of Bdellovibrio and wide application range, and can be used not only in the control of water source pathogen and food pathogen, but also in fields of medical science, environmental pollution, agriculture, industry and military matters.

Description

technical field

[0001] The invention belongs to the field of fermentation engineering, and specifically refers to a fermentation and cultivation technology of high-density Bdellovibrio swimming bodies that can be applied to reduce or even completely eliminate harmful bacteria, not only in the prevention and control of water-borne and food-borne pathogenic bacteria, but also It will have great application and development prospects in medicine, environmental pollution, agriculture, industry, military fields, etc. Background technique

[0002] Although the elimination of pathogenic bacteria by means of biological control is a research hotspot in various fields such as industry, agriculture, and medicine in the world today, the main means of eliminating pathogenic bacteria are still various physical and / or chemical methods, including various antibiotics. usage of. These methods all have obvious drawbacks. First, the excessive abuse of antibiotics will lead to some side effects,...