Production process of bdellophage preparation

A technology for phagocytosis of Bdellovibrio and production methods, applied in the direction of fungicides, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of difficult industrial production of Escherichia coli, environmental pollution, etc., and achieve disease prevention and control Improve the effect, avoid environmental pollution, and prolong the effect of shelf life

Inactive Publication Date: 2008-06-18
薛恒平 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a new production method of Bdellovibrio phage preparation, provide a new active host bacterium for the industrial production of Bdellovibrio phage, and solve the problem that the original Escherichia coli is difficult to carry out industrial production as the host bacterium. Cause the problem of environmental pollution, and ensure the lytic activity of Bdellovibrio bacteriophage, further improve its effect of disease control

Method used

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  • Production process of bdellophage preparation

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Effect test

Embodiment 1

[0014] Embodiment 1: 1. Preparation of host bacterium suspension——with the cultured photosynthetic bacterium Rhodopseudomonas suspension (see Chinese Journal of Microecology p.78.1990.3 for the culture method, and the bacterial strains include: Hunqiuhong pseudomonas Monospora P4 or Rhodopseudomonas palustris 101, bacterial classification purchased from Institute of Biotechnology, Shanghai Jiaotong University), let stand for 24 hours, remove the upper layer solution that accounts for 1 / 2 of the total solution volume; add and remove the upper layer solution equal volume Amount of 0.2% NaCl solution, shake well, and then stand still for 24 hours, remove the upper layer solution accounting for 1 / 2 of the total solution volume, then add 0.2% NaCl solution, and make the host bacteria suspension with 2 / 3 of the original total solution volume , the bacterial content is 3 to 30 billion / ml, and the obtained host bacterial suspension is used for future use.

[0015] 2. Preparation of cu...

Embodiment 2

[0017] Example 2: 1. Preparation of the host bacteria suspension - the cultured photosynthetic bacterium Rhodopseudomonas suspension (for the culture method, see China Microecological Impurities p. Monospora P4 or Rhodopseudomonas palustris 101, strains purchased from Shanghai Jiaotong University Biotechnology Institute), let stand for 48 hours, remove the upper layer solution that accounts for 2 / 3 of the total solution volume; add and remove the upper layer solution, etc. 0.75% NaCl solution by volume, shake well, and then let it stand for 48 hours, remove the upper layer solution accounting for 2 / 3 of the total solution volume, and then add 0.3% NaCl solution to make the host bacteria suspension of 1 / 2 of the original total solution volume solution, the bacterial content is 3 to 30 billion / ml, and the obtained host bacterial suspension is used for future use.

[0018] 2. Preparation of culture medium: Dilute PH7.5 phosphate buffer solution (can be PBS) 10 times with water, t...

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Abstract

The production process of bdellophage preparation includes the following steps: 1. preparing host bacteria suspension of live bacteria content 3-30 billion / ml with red pseudomonads suspension and 0.2%-0.85% concentration NaCl solution; 2. diluting pH 6.5-8.0 buffering phosphate solution and adding CaCl2 and MgCl2 in 10-100 mg / L to prepare the culture liquid; 3. adding host bacteria suspension in 5-30 vol% and bdellophage in 2-3 vol% to the culture liquid, and culturing at 28-30 deg.c for 72-120 hr to obtain product. The present invention is superior to colibacillus used as host bacillus with maybe environmental pollution, and has high bdellophage cracking activity and raised disease preventing and treating effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production method of a phage Bdellovibrio preparation. Background technique [0002] In aquaculture and animal disease prevention and control at home and abroad, chemical agents such as antibiotics are mainly used at present. The side effects of these preparations, such as drug residues, drug resistance of pathogenic bacteria, inhibition of beneficial bacteria, etc., have more and more adverse effects on human health and animal husbandry production. In recent years, the research and development of microbial ecological preparations has attracted the attention of all parties, especially the research on Bdellovibrio bacteriophage has been favored by people. [0003] Since Bdellovibrio phage was discovered in 1963, because it is a parasitic bacterium, it can crack Escherichia coli, Salmonella, Aeromonas hydrophila, Vibrio and other animal pathogenic bacteria, and it is har...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/74A61P31/04A01N63/00A01P3/00
Inventor 薛恒平
Owner 薛恒平
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