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Method for separating purified bdellovibrio from active sludge

An activated sludge, separation and purification technology, applied in the field of microorganisms, can solve problems such as prolonging the culture period and failure to isolate Bdellovibrio

Inactive Publication Date: 2015-01-28
SOUTHEAST UNIV
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Problems solved by technology

[0005] Conventional Bdellovibrio isolation methods will be affected by many factors during operation, such as inappropriate sample pretreatment means, the concentration of agar powder in the medium , the nutrients contained in the medium, the concentration of trace elements such as Ca2+ and Mg2+, pH, and the host bacteria and Its concentration, culture temperature, etc., if the conditions are not properly controlled, it will not only prolong the culture period, but even lead to the failure of Bdellovibrio isolation

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  • Method for separating purified bdellovibrio from active sludge

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Embodiment Construction

[0027] The present invention will be further described in conjunction with embodiment and accompanying drawing now.

[0028] The material source that the present invention relates to is as follows:

[0029] 1) The sludge samples were taken from the activated sludge in the secondary settling tank of the municipal sewage treatment plant.

[0030] 2) In the present invention, the used peptone yeast extract medium, diluted nutrient broth medium, and double-layer agar medium are adjusted to a certain extent on the basis of referring to the standard formula, and the pH of the medium is controlled at Between 6.8 and 7.5, the optimum pH is 7.1.

[0031] The proportion of peptone yeast extract medium is: peptone 9.5~10.5g / L, yeast extract 4.7~5.3g / L, sodium chloride 9.5~10.5g / L, agar powder mass concentration 1.0%~1.2%, ultra-pure Water 1000mL;

[0032]The ratio of 1 / 500 diluted nutrient broth culture medium is: ultrapure water 1000mL, peptone 9.5~10.5g / L, beef extract 2.8~3.2g / L, s...

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Abstract

The invention discloses a method for separating purified bdellovibrio from active sludge. By virtue of key techniques of host bacteria screening and active sludge pretreatment and technical steps such as preparation of a culture medium and a culture solution and optimization of culture conditions and the like, efficient bdellovibrio is separated and purified from a homologous sludge sample by optimization of a series of key steps by virtue of gram negative bacteria which are obtained in active sludge as host bacteria for separating and purifying bdellovibrio. The invention not only puts forwards a method for successfully separating the efficient bdellovibrio from the active sludge of a municipal wastewater treatment plant for the first time, but also the culture period of the method is remarkably shortened, so that the defects that the existing conventional method is unstable in bdellovibrio separating effect, long in culture period, tedious in operation and the like.

Description

[0001] technical field [0002] The invention belongs to the technical field of microbes, and relates to an optimized method for separating and purifying Bdellovibrio from activated sludge in a secondary sedimentation tank of a municipal sewage treatment plant. [0003] Background technique [0004] Bdellovibrio-like organisms ( Bdellovibrio- and-like organisms, referred to as Bdellovibrio) is a type of small parasitic bacteria that prey on host bacteria. Microporous filter membrane, the bacterium is arc-shaped or rod-shaped, it is an aerobic Gram-negative bacteria, it can invade suspended bacteria, and is effective against most families and genera of Gram-negative bacteria and a small number of Gram-negative bacteria in the environment. Positive bacteria have high lytic activity. Therefore, the unique ecological advantages of Bdellovibrio can be regarded as one of the important biological factors for the natural purification of the environment, and can be applied to the ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 余冉李传扬张诗文
Owner SOUTHEAST UNIV
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