Active biological antibacterial and production method thereof

An antibacterial substance and biological technology, applied in botany equipment and methods, biocides, antibacterial drugs, etc., can solve the problems of quality preservation, freshness preservation and removal of growth development and application restrictions, so as to inhibit the growth of cyanobacteria and avoid environmental pollution , high yield effect

Inactive Publication Date: 2011-03-30
秦生巨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, people use Bdellovibrio to prevent and control bacterial diseases of human body, animals and plants, and the development and application of fruits, veget...

Method used

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  • Active biological antibacterial and production method thereof
  • Active biological antibacterial and production method thereof
  • Active biological antibacterial and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one: the lysis effect of Bdellovibrio and phage mixed culture to bacteria of different species

[0027] 1. Source of strains: Bdellovibrio and phages are provided by the Vibrio phage professional laboratory of China Medical Bacteria Preservation Management Center; bacterial strains of different species are provided by China Institute for the Control of Pharmaceutical and Biological Products.

[0028] 2. Screening of Bdellovibrio strains and phage strains: Bd39, Bd40, Bd59, Bd76, Bd39, Bd40, Bd59, Bd76, Bd39, Bd40, Bd59, Bd76 and Bd81, Bd94, Bd98, Bd329, Bd334 and Vibrio phages ∮V3, ∮M6, ∮1a, ∮1b, Shigella phages ∮P22, ∮119X, ∮17, ∮ / F2a, typhoid phages ∮Vi, ∮S154 , pathogenic Escherichia coli phages ∮T2, ∮T7, Pseudomonas aeruginosa phages ∮PE5, ∮A, ∮B.

[0029] 3. Preparation of mixed culture of Bdellovibrio and phage:

[0030] Preparation of Bdellovibrio culture: see Chinese Journal of Microbiology and Immunology 1982, 2(1): 12-15. The obtained Bdellovibri...

Embodiment 2

[0044] Embodiment two: the protective effect of Bdellovibrio and phage mixed culture on guinea pig keratoconjunctival infection

[0045] 1. The source of the strain: provided by the Vibrio phage professional laboratory of the China Medical Bacteria Collection Management Center.

[0046] 2. Screening of Bdellovibrio strains and bacteriophage strains: screen Bdellovibrio sensitive to Shigella flexneri F2 a 301 (toxin-producing strain) from obtained Bdellovibrio strains and phage strains by conventional methods Bd98, Bd329 and phage ∮ / F2a.

[0047] 3. Preparation of mixed culture of Bdellovibrio and phage: use the bacterial strain screened in step 2 to prepare according to the method of Example 1.

[0048] 4. Preparation of Shigella flexneri culture: Shigella flexneri F2 a301 was isolated by streaking on a nutrient agar plate, cultured at 37 °C for 18 hours, and selected typical colonies were transferred to nutrient broth and cultured at 37 °C for 4- After 6 hours, a culture of...

Embodiment 3

[0056] Embodiment three: Bdellovibrio, bacteriophage mixed culture is to the cracking effect determination of Salmonella typhi

[0057] 1. Source of strains: Bdellovibrio and phage were provided by the Vibrio phage professional laboratory of China Medical Bacteria Collection Management Center; Salmonella typhi was provided by Jiangsu Provincial Center for Disease Control.

[0058] 2. Screening of Bdellovibrio strains and phage strains: Bd59, Bd81, Bd98, Bd125, Bd329, Bd125, Bd329, Bd125, Bd329, Bd125, Bd329 and Bd334, and Salmonella typhi phage ∮ / A, ∮ / D, ∮ / E, ∮ / K, ∮ / M1, ∮ / 36, ∮ / 53, ∮ / 96, ∮ / Vi.

[0059] 3. Preparation of mixed culture of Bdellovibrio and phage: the strains screened in step 2 were prepared according to the method in Example 1. The content of Bdellovibrio was 10 6 pfu / ml, the content of phage per milliliter is 10 9 pfu / ml.

[0060] 4. Preparation of Salmonella typhi culture: separate by streaking on a nutrient agar plate, culture at 37°C for 18 hours, select ...

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Abstract

The invention relates to active biological antibacterial and a production method thereof. The active biological antibacterial is prepared by proportionally mixing the cultures of bdellovibrio and bacteriophage serving as the main ingredients in the following steps: obtaining the bdellovibrio culture and the bacteriophage culture respectively, and preparing the cultures at a proportion of 1:1-1:1,000,000, namely that the bdellovibrio content is 10-1,010/pfu, and the bacteriophage content is 10-1,016/pfu; and mixing the cultures so that the cultures are suspended in medium liquid or mixed in excipient. The invention is used for preventing and controlling the bacterial diseases for human body, animals and plants, and has obvious effects on guaranteeing quality and freshness of fruits, vegetables, flowers, food and aquatic products, eliminating harmful bacteria in the environment and suppressing growth of blue-green algae.

Description

technical field [0001] The present invention relates to a biological agent for preventing and treating bacterial diseases of human body, animals and plants, preserving the quality and freshness of fruits, vegetables, flowers, food and aquatic products, removing harmful bacteria in the environment, and inhibiting the growth of cyanobacteria—active biological antibacterial substances and its production method. It belongs to the field of biological products and their manufacture. Background technique [0002] At present, the prevention and treatment drugs for bacterial diseases at home and abroad mainly include antibiotics and chemically synthesized antibacterial drugs. The use of such drugs, one is to continuously produce drug-resistant strains, and the effective dosage is gradually increased, causing the generation of "super bacteria" that are not sensitive to all antibacterial drugs; the other is to remain in the body, inhibiting the normal growth and metabolism of the body,...

Claims

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Application Information

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IPC IPC(8): A61K35/76A61P31/04A01N63/02A01P1/00A01P13/00A61K35/74
Inventor 秦生巨
Owner 秦生巨
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