46 results about "Redox indicator" patented technology

The present invention relates to a microbioligcal test method for detecting antibacterial compounds in liquid samples such as milk, meat juice, serum and urine. The method employs an acid-base or redox indicator to detect growth of a test organism and is characterised in that a thermophilic strain of Bacillus or Streptococcus, e.g. Bacillus stearothermophilus var. calidolactis C953, is employed at a concentration of above 107 CFU / ml, preferably between 2×107 and 3×108 CFU / ml.

The present invention relates to the analysis of the quality of functional fluids. In particular, the invention relates to using a stable redox indicator that produces a color at the presence of oxidation byproducts in a functional fluid.

A self-cure or dual-cure polymerizable dental composition is provided with a redox indicator. The composition exhibits an initial first color upon mixing of the two parts and then changes to a second color that is noticeably different from the first color during the curing or polymerization of the composition initiated through the redox initiator system. Advantageously, the first color may be distinctively different from tooth structure and can be easily noticed. The second color may be essentially colorless, a neutral color, or a non-visually observable color in reference to the tooth structure.

The invention discloses a preparation method of a compound electrochromic thin film. The method includes: adding water to a monomer, doping acid and an oxidation-reduction indicating agent to prepare a polymerization reaction solution, using an in-situ chemical oxidation polymerization method to compound the oxidation-reduction indicating agent with conductive polymer to obtain a conductive polymer / oxidation-reduction indicating agent compound electrochromic solution, and using the solution to form the electrochromic thin film on transparent conductive glass or a porous film through spin coating, spraying, lifting or printing film forming. The preparation method has the advantages that the color reaction of the oxidation-reduction indicating agent in the electrochromic process of the conductive polymer is utilized to improve the color changing performance of a conductive polymer electrochromic material, different macromolecule doping acids are used in the in-situ chemical polymerization reaction to obtain the water-soluble compound electrochromic solution, and the compound electrochromic thin film good in electrochromic performance is obtained finally.

A testing elements for the calorimetric determination of oxidizable gas and / or vapor components in gas mixtures is provided. The testing element contains, besides usual oxidizing agents, at least one redox indicator, which is in the oxidized form. The redox indicator is preferably a benzidine derivative according to the general formulain which the radicals R are identical or different and denote a hydrogen, alkyl, aryl, halogen, trifluoromethyl, cyano, nitro, dialkylamino, ester, sulfoxyl ester, alkyloxy or aryloxy, X denotes a halogen, Y denotes a trifluoromethyl, cyano, nitro, dialkylamino, ester, sulfoxyl ester, alkyloxy or aryloxy, n is an integer from zero to three, and m equals 5-n. The testing elements are preferably designed as detector tubes that contain carrier materials, to which the oxidizing reagents and redox indicators are applied.

The invention discloses an optical fiber and a manufacturing method and an identifying method of the optical fiber, and belongs to the field of the optical fiber. At least one coating layer of the optical fiber contains a color-developing indicator which is one of or combination of more than one of an acid-base indicator, a redox indicator and a complexing indicator. In the production process of the optical fiber, the color-developing indicator is added to the coating layer of the optical fiber so that the coating layer of the optical fiber is marked, and the optical fiber is further marked. A color-developing reaction is generated when the indicator meets a corresponding color-developing solution so that color of the color-developing solution develops. As other optical fibers do not possess the property, the optical fiber can be easily identified via the color-developing reaction so that marking and protection of the optical fiber are facilitated. The color-developing indicator is friendly to environment so that environmental pollution is not caused. The color-developing reaction is easy and rapid to operate and high in sensitivity. Color can develop with only a small amount of color-developing indicator so that performance of the coating layer of the optical fiber is not affected due to the small adding amount of the color-developing indicator.

This invention discloses a method for culturing anaerobic microbes. The method comprises: (1) preparing culture medium solution, and adding methylene blue as the redox indicator; (2) adding antioxidant into the culture medium solution at room temperature, shaking for dissolution, loading into a culture bottle, and covering with a rubber plug and a bottle lid within 30 min; (3) drilling the rubber plug with a syringe needle, introducing nitrogen, and exhausting at the same time to remove air above the solution in the culture bottle; (4) sterilizing the culture bottle with high-temperature steam, and cooling for further use. The method has such advantages as no need for anaerobic incubator easy operation and low cost. The redox indicator is still stable even after high-temperature sterilization. The antioxidant is safe and nontoxic, and can effectively remove oxygen.

A timing device for visually determining the passage of a preselected period of time including a redox indicator and a metal salt wherein the indicator undergoes an observable change in color which is related to the desired preselected period of time.

The present invention concerns methods and systems for learning or discovering redox-related context adjustments to a biological process or bioprocess experienced by one or more biological entities under local conditions. The bioprocess is postulated to have hidden states associated with redox reactions. Among other, the biological entities can be embodied by plants, animals, cells, cell cultures, cell lines and human subjects. The learning system uses a reference bioprocess model for the bioprocess and has a master learner configured to establish an observable basis of redox indicators for the bioprocess. The learning system also has a local learner in communication with the master learner. The local learner deploys a learning algorithm to learn an operator matrix that represents the redox-related context adjustment.

The invention relates to an indicator tube for indicating microbe growth and application thereof. As a general container, the indicator tube of the invention employs a drying method to conduct coloration for indicating storage and transportation of particles; so that indicating particles are maintained at a drying state be for usage, and influence of humidity on a redox indicator (e.g. tetrazole salt) is avoided, and the redox indicator is not susceptible to degradation or color changing. The invention also provides a kit for detecting microbe growth. The indicator tube of the invention is easily used, has good versatility, high sensitivity and low toxicity.

Provided is a method for measuring a color change of an oxidation-reduction indicator, which method is applicable to measurement of the cariogenic bacterial count and the like. A color change of an oxidation-reduction indicator is measured by a method for measuring a color change of an oxidation-reduction indicator, the method comprising reacting a test reagent with a test sample and measuring a color change, wherein the test reagent contains an oxidation-reduction indicator, an oxidation-reduction promoter, and a halogen salt.

The invention discloses an iodide ion detection kit based on iodine catalyzed arsenic-cerium reaction under the acidic condition and taking a Ferrion solution as a redox indicator. The kit comprises adigestion solution, a reducing agent, an oxidizing agent and an indicator; an ammonium persulfate or acidic potassium permanganate solution serves as the digestion solution; the arsenious acid solution serves as the reducing agent; a tetravalent cerium solution serves as the oxidizing agent; and a Ferroin reagent solution serves as the indicator. An iodine standard series solution is prepared, and the ammonium persulfate or acidic potassium permanganate solution is used as the digestion solution, so that the defects of dense smoke and explosiveness of chloric acid digestion are overcome. Thedetection kit also has the advantages of being simple, rapid, specified, sensitive and accurate, can be widely applied to the fields in which the content of iodide needs to be detected, and in particular, to urine iodine and water iodine detection in the fields of hygiene inspection and medical inspection.

The present invention relates to the domain of medical test technology, and is one kind of double phase blood substratum capable of making colony colorating. Into solid phase substratum, redox indicator tetrazolium red is added, so that the place with growing bacteria, exhibits carmine owing to the redox during bacteria growth; and nucleic acid and sodium glutamate in 0.2% concentration are also added to promote the growth of bacteria. The fast bacteria growth and the clony coloration make the present invention capable of detecting fast and simply in timely diagnosis.

The invention discloses a fiber-surface chemical plating technology used for a fiber grating sensor. The fiber-surface chemical plating technology includes the following steps: A, a fiber base body is preprocessed, and a preprocessed piece is obtained; and B, the preprocessed piece is arranged in plating liquid for chemical plating, wherein one liter of plating liquid comprises 9g to 12 g of blue vitriol, 15 g to 20 g of seignette salt, 25 g to 35 g of sodium hypophosphite, catalysts and oxidation-reduction indicators. According to the fiber-surface chemical plating technology, the surfaces of fibers are metalized in a chemical copper plating manner, and the temperature sensitivity and the strain sensitivity of the fibers are improved.

The invention relates to the technical field of microorganism detection, and particularly relates to a detection and counting method for the relative content of a microorganism. The method comprises the following steps: adding an oxidation-reduction indicator into a culture medium to obtain an indicator culture medium, wherein the color change interval of the oxidation-reduction indicator comprises three or more colors which can be easily recognized by naked eyes; diluting a sample to be detected, and culturing the diluted sample to be detected through the indicator culture medium, wherein multiple dilutions are used for the diluting operation, and multiple parallel samples are used for each dilution; reading the color or absorbency of the indicator culture medium during and / or the culturing operation; and obtaining the relative content of a microorganism in the sample to be detected according to the color change degree or absorbency change value of the indicator culture medium. Compared with the conventional MPN (most probable number) method, the method provided by the invention detects the relative contents of microorganisms in different samples more precisely and accurately; the comparability of the relative contents of microorganisms in different samples is higher; and the obtained data have favorable continuity. Thus, the method is wide in application range.

The slow-release formaldehyde-removing gel with the color-developing indication function comprises the following components in percentage by weight: 8-15% of a main agent consisting of one or more of sodium chlorite, potassium chlorite and magnesium chlorite; 15%-25% of a slow release agent composed of one or more of carrageenan, jelly glue, gel and agar; 5%-15% of a stabilizer composed of one or more of sodium chloride, magnesium chloride and potassium chloride; 0.05%-0.2% of a redox indicator composed of one of methylene blue, o-phenanthroline ferrous iron and sodium diphenylaminesulfonate; the main agent, the slow-release agent, the stabilizer and the oxidation-reduction indicator are dissolved in water and then are heated and coagulated into gel, and the component A is formed; 4%-8% of an activating agent composed of an aqueous solution of one or more of sodium hydrogen sulfate, sodium sulfate, potassium hydrogen sulfate, potassium sulfate, citric acid and EDTA (Ethylene Diamine Tetraacetic Acid); the activating agent is a component B; when not in use, the component A and the component B are packaged in an isolated manner.

The present invention concerns methods and systems for learning or discovering redox-related context adjustments to be applied to model conditions, e.g., in a laboratory, in which a reference biological entity is undergoing the bioprocess. A reference bioprocess model that may be used under field or local conditions is constructed based on the reference biological entity's experience of the bioprocess. The bioprocess is postulated to have hidden states associated with redox reactions. Among other, the reference biological entity may be a model cell line set up to undergo the bioprocess in vitro. A mechanism is provided for perturbing the model conditions to transition from a baseline redox-related context to a perturbed redox-related context. Redox-related context change is learned using operator matrices that transform model feature vectors containing redox indicators from baseline to perturbed redox-related context.

A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.

The invention discloses a method for detecting Fe<3+> and GSH by a fluorescence-colorimetric dual-signal sensor based on a MoSe2@Fe nano composite material; the fluorescence intensity of MoSe2@Fe is inhibited by Fe<3+> through adding Fe<3+>, the fluorescence intensity of the material is recovered after GSH is added, and a fluorescence sensor of the MoSe2@Fe nano composite material is constructed; a standard curve is established according to the fluorescence intensity measured by a fluorophotometer to detect the concentrations of Fe<3+> and GSH; TMB is used as a redox indicator for colorimetric use, and after Fe<3+> is added, the Fe<3+> enhances the enzymatic activity of the nano composite material, so that the blue color is deepened; and then the GSH is added, the GSH can directly reduce the oxTMB, the blue color becomes light, and thus the concentrations of Fe<3+> and the GSH are visually detected. The method has the advantages of time saving, labor saving, low detection limit, good accuracy and simplicity and rapidness in detection.

In the present invention, according to the spoilage microorganism growth kinetic information of the refrigerated food, a time and temperature indicator capable of monitoring the microbial spoilage ofthe refrigerated food is developed by coupling reaction kinetics and microbial spoilage kinetics of the refrigerated food. The time-temperature indicator comprises a low temperature resistant microorganism, a microorganism growth support substance, a microorganism growth indicator comprising a redox indicator and / or a pH indicator, and a microorganism growth influencing substance (e.g., alcohol) that can influence microorganism growth kinetics.

The present invention concerns methods and systems for learning about the redox status of a biological process experienced by a biological entity under local conditions by postulating hidden states that are locally inaccessible but are related to the redox status. The learning system uses a reference bioprocess model from which a master learner establishes an observable basis of redox indicators that are real-valued, measureable, and exclude hidden states. Meanwhile, a local learner receives at least a portion of model redox data from the reference bioprocess model, and measured redox data from the biological entity. The learning system runs a distributed learning algorithm using the observable basis established by the master learner to determine an optimal composition of redox data to be included in measured redox data collected under local conditions.

The invention concerns methods and reagent kits for the fluorimetric determination of analytes.

This invention discloses a method for culturing anaerobic microbes. The method comprises: (1) preparing culture medium solution, and adding methylene blue as the redox indicator; (2) adding antioxidant into the culture medium solution at room temperature, shaking for dissolution, loading into a culture bottle, and covering with a rubber plug and a bottle lid within 30 min; (3) drilling the rubberplug with a syringe needle, introducing nitrogen, and exhausting at the same time to remove air above the solution in the culture bottle; (4) sterilizing the culture bottle with high-temperature steam, and cooling for further use. The method has such advantages as no need for anaerobic incubator easy operation and low cost. The redox indicator is still stable even after high-temperature sterilization. The antioxidant is safe and nontoxic, and can effectively remove oxygen.

An electrochemical DNA biosensor comprising a screen-printed electrode modified with gold nanoparticles, a layer of silica nanosphere composite, an oligonucleotide sequence, a cross-linking agent anda redox indicator. The layer of silica nanosphere composite is deposited on the screen-printed electrode and the oligonucleotide sequence is immobilized on the layer of silica nanosphere composite using the cross-linking agent. Further, at least a portion of the oligonucleotide sequence is complementary to a unique target sequence of a nucleic acid from a fish of interest such that the nucleic acid from the fish of interest hybridizes to the oligonucleotide sequence to form a dsDNA. Furthermore, the redox indicator intercalates with the dsDNA which enables detection of gender and variety of the fish by voltammetry.

A method for detecting a bacterial load comprising adding a sample for analysis to an analysis reagent in a sterilized reaction container, thermostatting the reaction container at a temperature of between 25 and 45° C., and verifying the change in colouring of the analysis reagent. The analysis reagent is an aqueous solution comprising amino acids chosen from the group consisting of meat peptones, vegetable peptones, casein hydrolysates, tryptose, tryptones and yeast extract; glucides chosen from monometric or oligomeric glucides metabolisable by micro-organisms; a buffer system suitable for maintaining the pH between 5.5 and 8.5; a redox indicator with potential between −250 and +250 mV and / or a pH indicator with colour change interval between pH 4.0 and pH 9.0; and a water-immiscible organic liquid compound having a lower density than water and suitable for separating the aqueous phase from a gaseous phase existing prior to the analysis or formed during the reaction.

A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a [beta]-D-glucuronidase indicator system that comprises a plurality of compounds thatenhance [beta]-glucuronidase enzyme activity in Escherichia coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using thedevice are also provided.