Rapid test method of toxicity in marine oil spill organisms

A biotoxicity and detection method technology, applied in the direction of chemiluminescence/bioluminescence, analysis of materials through chemical reactions, bacteria, etc., can solve the problems of changing the salinity of luminescent bacteria and affecting the results, so as to reduce errors and improve detection results scientifically proven results

Inactive Publication Date: 2009-10-07
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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Problems solved by technology

At present, my country has formulated the water quality testing standard for luminescent bacteria (GB / T154411995). Some scholars at home and abroad have also tested the biological toxicity of some oil products through luminescent bacteria, but because the luminescent bacteria used in the experiment are extracted from the ocean However, in the previous detection process, pure water was used as the solvent for the preparation of water fusion components, which changed the salinity of the luminescent bacteria and affected the results to a certain extent.

Method used

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  • Rapid test method of toxicity in marine oil spill organisms
  • Rapid test method of toxicity in marine oil spill organisms
  • Rapid test method of toxicity in marine oil spill organisms

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Embodiment Construction

[0035] 1. Cultivation of Luminescent Bacteria

[0036] The strain is Photobacterium phosphoreum T3 variant (Photobacterium phosphoreum) to prepare fresh bacterial suspension of Photobacterium phosphoreum:

[0037] (1) Cultivation of slant strains: Take the first-generation slant on a fresh slant, and immediately transfer to the second-generation slant after culturing at 25°C±0.5°C for 24 hours, and then transfer to the third-generation slant after culturing at 25°C±0.5°C for 24 hours , 25 ℃ ± 0.5 ℃ cultured for 24 hours before use.

[0038] (2) Bacterial liquid culture in shake flasks: take nearly one ring of the third-generation slant strain, inoculate it in a 100ml Erlenmeyer flask filled with 30ml of bacterial culture solution, and incubate for 24 hours at 25°C±0.5°C and 160r / min for later use.

[0039] (3) Dilute the cultured luminescent bacteria suspension to 10 per milliliter 8 a ~ 10 9 cells, the initial luminosity is not lower than 800mV, and it is reserved.

[004...

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Abstract

The invention relates to a rapid test method of toxicity in marine oil spill organisms. The method takes photobacteria as tested organisms for testing the toxicity in the marine oil spill organisms and simultaneously utilizes artificial seawater for preparing water accommodation friction (WAF), thus avoiding the experiment error caused by the reduction of the salinity of marine photobacteria and the changes of living conditions of the photobacteria due to the application of pure water as solvent for preparing the WAF in previous photobacteria experiments.

Description

technical field [0001] The invention relates to a rapid detection method for marine oil spill biological toxicity. Background technique [0002] With the acceleration of the industrialization process, people are more and more dependent on oil, which increases the density of oil tankers in ports and coastal areas, complicates the already busy navigation environment, and increases the risk of oil spill pollution from ships. The oil spill incidents in recent years have caused a large area of ​​marine pollution and brought huge losses to the ecological environment of the sea area, which has attracted people's attention. At present, the detection of oil toxicity largely relies on chemical means and animal experiments, but this method is expensive, requires many animals, and is time-consuming. Therefore, in recent years, a fast and simple method is preferred, and the toxicity test method of luminescent bacteria is one of the biological detection methods that meet these requiremen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76C12N1/20
Inventor 刘长安张世宇
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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