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Thin-layer chromatography coloration method

A technology of thin-layer chromatography and color development, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of unclear structure, small content, and difficulty in color development of unknown antibiotics, achieving obvious effects, easy operation, and application wide range of effects

Inactive Publication Date: 2006-03-08
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0003] When using thin-layer chromatography to separate unknown antibiotic substances, because the structure of the unknown antibiotic substances is not clear and the content is very small, the antibiotic itself does not produce fluorescence under ultraviolet light, and its color development is difficult.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Screening of marine bacteria producing unknown antibiotics

[0021] Seawater and sea mud samples were selected, diluted with sterile water, and coated with Zobell 2216E medium to separate and purify marine bacteria. Candida albicans was used as the test bacteria, and the inhibitory effect of purified marine bacteria on Candida albicans was measured by digging method, and a strain of marine bacteria with inhibitory effect was obtained.

[0022] (2) Fermentation culture of marine bacteria and preliminary purification of anti-living active substances

[0023] After the marine bacteria are fermented and cultivated, some impurities in the fermented liquid are removed by acid precipitation and ion exchange, and the unknown antibiotic active substances produced by the fermentation are preliminarily purified.

[0024] A.Material:

[0025] 1. Strains

[0026] (1) 71 strains of marine spores were isolated from coastal samples in China.

[0027] (2) Candida albicans, clini...

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Abstract

This invention refers to thin layer chromatography color development method, which contains 1, laying the dissolved biological color development culture medium on chromatography thin board air dried by normal method, 2, preparing test organism into bacteria suspension liquid and coating on biological color development culture medium, 3, putting the bacteria coated board in culture box to culturing color development.

Description

technical field [0001] The invention relates to thin-layer chromatography, specifically a thin-layer chromatography color development method for separating unknown antibiotic active substances by thin-layer chromatography. Background technique [0002] Thin layer chromatography (TLC) is a method of separating components of a mixture based on their uneven distribution or retention between stationary and mobile phases. Thin-layer chromatography is to spread the stationary phase on the support plate so that it forms a uniform thin layer. The sample solution to be separated is placed on the thin-layer plate at a position about 10mm away from the lower edge, and the lower edge is placed downward into a closed cylinder filled with a suitable mobile phase with a depth of about 5mm for chromatographic development. This achieves the separation of the mixture. The developed chromatographic bands (spots) can be qualitatively detected and quantitatively determined by appropriate techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94
Inventor 马成新郭敬斌胡江春薛德林王书锦
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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