Thin-layer chromatography coloration method
Patent Information
- Authority / Receiving Office
- CN Β· China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
- Publication Date
- 2006-03-08
- Estimated Expiration
- Not applicable Β· inactive patent
Abstract
Description
technical field
[0001] The invention relates to thin-layer chromatography, specifically a thin-layer chromatography color development method for separating unknown antibiotic active substances by thin-layer chromatography. Background technique
[0002] Thin layer chromatography (TLC) is a method of separating components of a mixture based on their uneven distribution or retention between stationary and mobile phases. Thin-layer chromatography is to spread the stationary phase on the support plate so that it forms a uniform thin layer. The sample solution to be separated is placed on the thin-layer plate at a position about 10mm away from the lower edge, and the lower edge is placed downward into a closed cylinder filled with a suitable mobile phase with a depth of about 5mm for chromatographic development. This achieves the separation of the mixture. The developed chromatographic bands (spots) can be qualitatively detected and quantitatively determined by appropriate techn...