Thin-layer chromatography coloration method

A technology of thin-layer chromatography and color development, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of unclear structure, small content, and difficulty in color development of unknown antibiotics, achieving obvious effects, easy operation, and application wide range of effects
CN1743842AInactive Publication Date: 2006-03-08SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Publication Date
2006-03-08
Estimated Expiration
Not applicable Β· inactive patent
Patent Text Reader

Abstract

This invention refers to thin layer chromatography color development method, which contains 1, laying the dissolved biological color development culture medium on chromatography thin board air dried by normal method, 2, preparing test organism into bacteria suspension liquid and coating on biological color development culture medium, 3, putting the bacteria coated board in culture box to culturing color development.
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Description

technical field

[0001] The invention relates to thin-layer chromatography, specifically a thin-layer chromatography color development method for separating unknown antibiotic active substances by thin-layer chromatography. Background technique

[0002] Thin layer chromatography (TLC) is a method of separating components of a mixture based on their uneven distribution or retention between stationary and mobile phases. Thin-layer chromatography is to spread the stationary phase on the support plate so that it forms a uniform thin layer. The sample solution to be separated is placed on the thin-layer plate at a position about 10mm away from the lower edge, and the lower edge is placed downward into a closed cylinder filled with a suitable mobile phase with a depth of about 5mm for chromatographic development. This achieves the separation of the mixture. The developed chromatographic bands (spots) can be qualitatively detected and quantitatively determined by appropriate techn...

Claims

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