Waste water online biological monitoring method and device
A biological monitoring and waste water technology, applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems that cannot be applied in the field environment, the system can only be used seasonally, and the installation and operation costs are high, so as to achieve low cost, Easy to operate and produce convenient effects
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Embodiment 1
[0057] Indoor test:
[0058] Instruments and devices used:
[0059] The wastewater on-line biological monitoring device includes two sets of channel units, each unit includes a circulation pump 3, a liquid storage chamber 2, a flow chamber 1, an induction electrode 4 and a multimeter 5, and the circulation pump is placed at the bottom of the side of the liquid storage chamber away from the flow chamber, The flow chamber is connected to the flow chamber through a plastic tube, and the side wall of the flow chamber is connected to the side of the liquid storage chamber at the same height to realize the full flow exposure. Two pairs of copper electrodes with nuts are placed on the inside of the flow chamber, one pair of which is connected to the regulated power adapter As an electrical signal input end, the other pair of electrodes is connected with a multimeter through wires to form an electrical signal output end. Design and customize 12 2×8×10cm containers (with lids) made of...
Embodiment 2
[0071] Indoor test:
[0072] Instruments and devices used:
[0073] The basic structure of the waste water on-line biological monitoring device is the same as that in Example 1. 12 containers (with lids) of 2×8×10cm made of polymethyl methacrylate are designed and customized, and coordinate paper is pasted on one side of the container. The height difference is 5mm (such as Figure 13 500mL volumetric flask x 6, 100mL graduated cylinder x 1, pipette gun, pH meter, electronic balance, aeration device and constant temperature light incubator, etc.
[0074] chemical:
[0075] p-Nitrophenol (chemically pure), DMSO (analytical pure), methanol, calcium oxide, magnesium sulfate, sodium bicarbonate, potassium chloride, etc.
[0076] Experimental steps:
[0077] (A) Solution preparation: Accurately weigh 0.010g of p-nitrophenol, dissolve it with dilution water, place it in a 500mL volumetric flask, and dilute to volume with dilution water to obtain a solution with a concentration of...
Embodiment 3
[0086] Indoor pilot test:
[0087] Devices used: such as Figure 7
[0088] Experimental steps:
[0089] (A) High concentration stock solutions of two compounds (PFOA1600 mg?L) were prepared before the flow exposure experiment -1 and PFOS 800 mg?L -1 ), using no more than 0.5‰ of DMSO to aid dissolution.
[0090] (B) Add 250mL of artificially diluted aqueous solution to the reference and experimental flow-through chambers, and then put 10 adult fleas in each flow-through chamber.
[0091] (C) After the Daphnia magna was placed in the circulation chamber for 5 minutes, add 250 mL (containing 0.5‰ DMSO) of dilution water to the reference circulation chamber and 250 mL of dilution water containing PFOA to the experimental circulation chamber, and turn on the switch of the power adapter. Simultaneously start the experiment timing.
[0092] (D) Starting from time 0, the instantaneous induced currents of the reference and experimental flow chambers were recorded every 5 min ( ...
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