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Compound microbial degradation agent for organic pollutants and preparation method of compound microbial degradation agent

A technology of organic pollutants and composite microorganisms, which is applied to the degradation of organic waste by biological materials and/or photocatalytic materials, and the treatment of organic pollutants, and can solve the problems of limited use of photocatalysts and limited application environments

Inactive Publication Date: 2018-09-28
佛山市尚柏科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of photocatalytic degradation also has many disadvantages: firstly, its application environment is limited, and photocatalysts usually need to exert photocatalytic effects to perform photocatalytic degradation, and generate electron holes through ultraviolet light or visible light irradiation, thereby generating strong reactive free radicals
Therefore, in soil or darker environments, the use of photocatalysts is limited

Method used

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  • Compound microbial degradation agent for organic pollutants and preparation method of compound microbial degradation agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 prepares nano titanium dioxide

[0028]First, 30mL of water and 120mL of ethanol were mixed in a volume ratio of 1:4, and acid or alkali was added to adjust the pH of the solution to obtain a solution as a reaction medium, and then 18mL of the precursor tetra-n-butyl titanate (TBT) was mixed with ethanol, As a reaction precursor, add it dropwise to a mixed solvent of water and ethanol at 75°C, and continue to react at 75°C for 24 hours after the dropwise addition is completed, and perform high-speed centrifugation on the obtained suspension, wash with water and ethanol, and dry Afterwards, nano-titanium dioxide powder is obtained.

Embodiment 2

[0029] Embodiment 2 prepares bacterial powder

[0030] 1. Preparation of Bacillus bacteria powder

[0031] a. Slant culture: inoculate the original strains of Bacillus subtilis and Bacillus megaterium on the slant medium under sterile conditions, and cultivate them at 30±2°C for 24 to 36 hours;

[0032] b. Shaker culture: Inoculate the strains cultured in step a into the seed medium under sterile conditions, and culture on a shaker at 160-180r / min for 12-18 hours at pH 7.0-7.5 and 30°C ;

[0033] c. Fermentation tank culture: Inoculate the strains cultivated in step b into the liquid fermentation medium under sterile conditions respectively, at pH 7.0-8.0, tank pressure 0.5kg / cm 2 , 30 DEG C, ventilation rate 1:0.8~1.1 under the conditions, after cultivating for 48~72 hours, when 80% of the bacteria become spores, put into the tank to obtain the fermentation liquid;

[0034] d. After concentrating the fermented liquid obtained in step c, mix and dry it with carrier chaff po...

Embodiment 3

[0043] Embodiment 3 prepares organic pollutant composite microbial degradation agent

[0044] Weigh bacterial powder, vegetable oil, BHA according to parts by weight, and slowly mix them in a blender evenly to obtain treated bacterial powder or spore powder; mix other auxiliary materials to obtain adjuvant mixture; prepare according to the weight ratio of Example 1 The obtained nano-titanium dioxide is mixed with the bacteria powder, then the auxiliary agent mixture is added, stirred evenly in a mixer, and then dried, screened and packaged to obtain a composite microbial degradation agent for organic pollutants.

[0045] Concrete preparation obtains following product (characterize each component consumption with weight percentage):

[0046] Product 1: Bacillus subtilis powder (preservation number is CGMCC NO.1.9083, about 100 million cfu / g) 5%, nano-titanium dioxide 30%, BHA 1%, grape seed oil 9%, glucose 15%, light calcium carbonate 20%, sodium dodecylbenzene sulfonate 5%, s...

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Abstract

The invention relates to the field of environmental protection, and relates to degradation of organic waste by using biological materials and / or photocatalytic materials. Microbial strains are selected from one or more of Bacillus subtilis, Bacillus megaterium and Aspergillus sydowii, wherein Bacillus subtilis with a preservation number of CGMCC No.1.9083 is preferably selected for the Bacillus subtilis, Bacillus megaterium with a preservation number of CGMCC No.1.16094 is preferably selected for the Bacillus megaterium, and Aspergillus sydowii with a preservation number of CGMCC No.3.13937 ispreferably selected. A photocatalyst nano-titanium dioxide is adopted to cooperate with the microorganisms, a very good result is achieved in degradation of pesticide, especially in degradation application of methamidophos, methyl parathion and triazophos.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to the treatment of organic pollutants (such as pesticides and chemical wastes) in the environment, and further relates to the degradation of organic waste by biological materials and / or photocatalytic materials. Background technique [0002] With the rapid development of industrialization, a large amount of waste will inevitably be generated in the process of industrial or agricultural production, such as organic waste discharged from chemical plants, pesticide residues in farmland, and pesticides that eventually flow into water bodies with rainwater. With our awareness and increasing emphasis on environmental protection, the treatment of the above pollutants has become particularly important. [0003] Chemical pesticides are synthetic xenobiotics. Many pesticides are toxic to humans or other organisms, and many types of pesticide residues are difficult to degrade. This has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/34C02F1/30C02F1/725C02F2101/306C02F2305/10
Inventor 李锦成
Owner 佛山市尚柏科技有限公司
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