Method for degrading pyrethrins pesticide by magnetic polyurethane foam plastic immobilized cell
A technology of foamed plastics and pyrethroid pesticides, applied to chemical instruments and methods, fixed on/in organic carriers, fibrous fillers, etc., can solve the problem of large losses
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Embodiment 1
[0024] Example 1: Magnetic polyurethane foam immobilized JCX22D cells to degrade permethrin pesticides in water
[0025] Beta-cypermethrin, cyhalothrin, deltamethrin, and bifenthrin are dissolved in acetone and added to sterile water at a ratio of 20 mg / l. Put the foam block of fixed JCX22D strain cells in the pesticide water body. Incubate at 30°C for 108 hours, extract the pyrethroid pesticide in the water sample with cyclohexane, and measure the pesticide residue by gas chromatography (Trace GC), and the detector is an electron capture device (ECD). Sterile water without pesticides was used as a control. The measurement results are shown in Table 1.
[0026] Table 1 Degradation rate of four pyrethroid pesticides in water by immobilized JCX22D strain (%)
[0027] permethrin
Embodiment 2
[0028] Example 2: Magnetic polyurethane foam immobilized JCX22D cells to degrade organophosphorus pesticides in water
[0029] Four organophosphorus pesticides, phoxim, methyl parathion, chlorpyrifos and diazinon, were dissolved in acetone, and added to sterile water at a ratio of 20 mg / l. Put the foam blocks that fix the JCX22D strain cells in the water body. Incubate at 30°C for 108 hours, extract pesticides in water samples with cyclohexane, and perform wavelength scanning with UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer, and determine the degradation rate of pesticides by comparing the samples with the control. With sterile water without pesticides as a control, the results are shown in Table 2.
[0030] Table 2 Degradation rate of four organophosphorus pesticides in water by immobilized JCX22D strain (%)
[0031] organophosphate
Embodiment 3
[0032] Example 3: Magnetic polyurethane foam immobilized JCX22D cells degrade avermectin pesticides in water
[0033] Abamectin is dissolved in acetone, and the foam block for fixing JCX22D strain cells is put in the pesticide-containing water body according to the ratio of foam block to water body area ratio of 1:10. Incubate at 30°C for 108 hours, extract abamectin in water with trichlorohexane, and perform wavelength scanning with a UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer, and determine the degradation rate of the pesticide by comparing the sample with the control. The soil samples without abamectin and sterile water were used as controls. The measurement results are shown in Table 3.
[0034] Table 3 Degradation rate (%) of immobilized JCx22D strain to avermectin pesticide in water
[0035] Control concentration (mg / L)
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