Time-resolved fluorescence immunoassay kit detecting methyl parathion and detection method thereof

Abstract

The invention discloses a time-resolved fluorescence immunoassay kit detecting methyl parathion (MP) and a detection method thereof. The time-resolved fluorescence immunoassay kit detecting methyl parathion is composed of a porous coating plate, a buffer, a methyl parathion standard sample, a methyl parathion antibody freeze-dried sample, an europium-labelled goat anti-mouse antibody, a washing liquid and an enhancing liquid. The detection method employing the time-resolved fluorescence immunoassay kit detecting methyl parathion comprises the following steps: (1) preparing an immunogen; (2) preparing a coating antigen; (3) preparing a monoclonal antibody; and performing pretreatment and detection on a sample. The kit and the method are short in detection time, high in average recovery rate, simple for sample pretreatment, capable of realizing on-site operation detection, wide in application scope and low in detection cost, and also possess the advantages of being strong in detection specificity, small in intra- and inter-assay difference, high in sensitivity, simple and rapid in operation, especially suitable for detection of a large bath of samples, and the like.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a time-resolved fluorescent immunoassay kit for detecting methyl parathion (MP) and a detection method thereof. Background technique [0002] Parathionmethyl, also known as O,O-dimethyl-O-p-nitrophenylphosphorothioate, is a highly toxic organophosphate insecticide with large output and wide application. It is highly toxic and has been banned from use on vegetables and fruits in recent years. However, indiscriminate use and abuse of pesticides still exist in vegetable production, and methyl parathion (MP) is still the focus of routine testing in agricultural product import and export trade and food safety supervision and testing. [0003] Methyl parathion (MP) residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC / MS). These methods are sensitive and accurate, and can simul...

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Problems solved by technology

[0003] Methyl parathion (MP) residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC / MS). These methods are sensitive and accurate, and can simultaneously determine multiple It is a kind of drug, but it needs expensive instruments, the sample pretreatment is complex, tedious and time-consuming, the detection cost is high, and it needs to be operated by professionals, it is difficult to meet the needs of on-site, batch and rapid detection of samples

There are currently no patents and literature reports on a chronofluoroimmunoassay for the detection of methylparathion (MP)

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