Method for measuring nanomolar reactive phosphate in sea water

A phosphate and seawater technology, applied in the direction of measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of time-consuming and labor-intensive, large sample volume, etc., and achieve the effect of cheap price, low detection cost, and easy automation

Inactive Publication Date: 2009-07-08
XIAMEN UNIV
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  • Application Information

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Problems solved by technology

However, this method consumes a large sample volume, and is time-consuming and labor-intensive. When quantitatively determining samples with a phosphorus content below 30nmol / L, generally more than 500mL of water samples are required, and it takes 7h

Method used

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  • Method for measuring nanomolar reactive phosphate in sea water
  • Method for measuring nanomolar reactive phosphate in sea water

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Experimental program
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Effect test

Embodiment 1

[0014] Standard curve drawing: Take 150mL series standard solutions containing phosphorus respectively 0, 3.4, 10.3, 20.6, 30.9, 61.9, 123.7nmol / L, and mix them with 2.1mL ammonium molybdate mixed solution (the preparation method is the same as the marine monitoring specification) and 2.1 Ascorbic acid solution with a mL concentration of 100g / L was mixed and reacted at room temperature for 2min. The reacted solution was passed through the HLB column at a speed of 21.0mL / min by a peristaltic pump for extraction and enrichment. The enrichment volume was 105mL; Wash the HLB column with 4mL concentration of 0.15mol / L sodium chloride solution, and then use 3.0mL concentration of 0.15mol / L sodium hydroxide solution to elute the extract at a speed of 5.6mL / min, and use a spectrophotometer at 740nm The detection was carried out, and the absorbance value was recorded by computer software. Make a working curve of phosphate concentration and absorbance: A=0.011+0.00606C P (n=7, R 2 =0....

Embodiment 2

[0019] With the method of the present invention, the water samples collected from the Southeast Asia Time Series Station (SEATS station) in the South China Sea were measured in July 2007. The water samples were shallower than 100m. The water samples were stored frozen in the refrigerator before analysis, and the content of active phosphorus was analyzed immediately after thawing thoroughly, and each sample was measured 1 to 3 times. The result is as figure 1 , which is consistent with results obtained by other oceanographers.

[0020] 1) Mix a certain concentration of phosphate seawater spiked solution with the following reagents at a volume ratio of 50-150:1, ammonium molybdate mixed solution and reducing agent solution, react in a water bath at 30-50°C for 0-20 minutes, and use infusion The device extracts and enriches the reacted solution through the enrichment column at a speed of 7-25mL / min, with an enrichment volume of 0-200mL; sequentially washes and enriches with 0-5m...

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Abstract

The invention discloses a method for determining nanomolar active phosphate in seawater, and relates to a method for determining active phosphate with the concentration as nanomolar grade in the seawater. The invention provides the method for determining the nanomolar active phosphate in the seawater which is sensitive, accurate and quick, and is easy for field analysis on ships. The seawater with phosphate is added with a standard solution and is mixed with a reagent; water and a pre-washing solution are used to wash an enriching column after extraction and enrichment; then an extracted substance is eluted; a detector is used to detect at a position between 650 and 850nm; a corresponding signal is recorded; a working curve of the concentration of the phosphate and the corresponding signal is worked out; an actual seawater sample is mixed with the reagent, the water and the pre-washing solution are used to wash the enriching column after the extraction and the enrichment; then the extracted substance is eluted; the detector is used to detect at the position between 650 and 850nm; the corresponding signal is recorded; the standard working curve is used to work out the concentration of the phosphate in the seawater; and the determined concentration of the phosphate is the concentration of the active phosphate according to a definition of the active phosphate.

Description

technical field [0001] The invention relates to a method for determining the concentration of nanomole active phosphate in seawater. Background technique [0002] Phosphorus is an essential nutrient element for the growth of marine organisms. Due to the massive consumption of phytoplankton in the ocean surface, the concentration of phosphorus is very low, and even becomes a limiting element for the primary productivity of the ocean. With the in-depth research on the biogeochemical cycle of phosphorus, the analysis and determination method of phosphate, which is the main component of phosphorus in seawater, has become the key to the scientific research of marine environment. Phosphorus molybdenum blue (PMB) spectrophotometry is the most commonly used classic determination method of phosphate in current marine monitoring regulations, which stipulates that the part of phosphorus that can be measured by phosphorus molybdenum blue spectrophotometry is active phosphorus, including...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N30/08
Inventor 马剑袁东星梁英陈国和
Owner XIAMEN UNIV
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