Preparation method for lipoic acid polymer impurities and detection method for lipoic acid polymer impurities
A technology of d-lipoic acid and its composition, which is applied in the field of preparation and detection of lipoic acid polymer impurities, can solve the problems of undetectable polymer impurities, adverse reactions, curative effect and safety hazards, etc., and achieves simple operation, Easy to obtain raw materials and high detection sensitivity
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[0051] Example 1 Preparation of dextro-lipoic acid oligomer
[0052] Dissolve 10g of dextro-lipoic acid monomer in 200mL of anhydrous dioxane, add 0.5g of azobisisobutyronitrile in catalytic amount, stir at 35-40℃ for 15min, then quickly cool to 10-15℃ In the meantime, keep stirring for 1h, add about 10mL of 5% dilute hydrochloric acid to terminate the reaction; put the reaction solution into a mixture of 400mL ethyl acetate and 400mL water, stir and separate into layers, take the ethyl acetate layer, and then use ethyl acetate for the water layer Extract twice with 200mL, combine the organic layers, concentrate at least the volume of about 50mL, add 300mL of isopropyl ether, precipitate a pale yellow semi-solid, filter, and vacuum dry at 30±2℃ for 10h to obtain the above-mentioned dextro-lipoic acid oligomer The composition is about 7.5g, the yield is 75%, and the HPLC purity is 98.5%.
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[0053] Example 2 Purification of dextro-lipoic acid oligomer
[0054] Suspend 5 g of the crude dextro-lipoic acid oligomer obtained in Example 1 in 40 mL of methyl isoamyl ketone, stir and disperse, raise the temperature to between 45-50°C, and slowly add ethanol / water with a volume ratio of 1:1 The mixed solvent is about 15mL, keep warm and stir until it is completely dissolved, filter while hot, slowly reduce the temperature to 0-5℃, collect the precipitated light yellow semi-solid, and dry it under vacuum at 30±2℃ for 12h to obtain 3.5g refined product with purity of 99.5%.
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[0055] Example 3 Preparation of refined product of dextro-lipoic acid oligomer
[0056] Dissolve 8g of dextro-lipoic acid monomer in 180mL of anhydrous tetrahydrofuran, add 0.4g of azobisisobutyronitrile in a catalytic amount, stir at 35-40℃ for 20min, then quickly cool down to 10-15℃ and keep warm Stir for 1 hour, add 20 mL of 5% dilute phosphoric acid to terminate the reaction; put the reaction solution into a mixture of 400 mL of ethyl acetate and 400 mL of water, stir to separate the layers, take the ethyl acetate layer, and extract the water layer twice with 200 mL of ethyl acetate. Combine the organic layers, concentrate at least the volume of about 40mL, add 250mL of isopropyl ether, precipitate a pale yellow semi-solid, filter, and vacuum dry at 30±2°C for 12h to obtain the composition of the dextro-lipoic acid oligomer, about 5.9 g, the yield is 78%, and the HPLC purity is 98.6%; the resulting product is further refined, that is, the above 5g sample is suspended in 45 mL...
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