Preparation of chlorpyrifos and methyl parathion universal antibody and universal envelope antigen

A technique of methyl parathion and coating antigen, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of the simultaneous enzyme-linked immunosorbent assay kit and its preparation method, etc., and achieves a simple pretreatment process, The effect of increasing sensitivity

Inactive Publication Date: 2009-07-08
孙家隆
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Problems solved by technology

Current literature and patents disclose ELISA kits for the analysis of residual chlorpyrifos or enzyme-linked immunosorbent assay kits for the analysis of residual methyl parathion

Method used

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  • Preparation of chlorpyrifos and methyl parathion universal antibody and universal envelope antigen
  • Preparation of chlorpyrifos and methyl parathion universal antibody and universal envelope antigen
  • Preparation of chlorpyrifos and methyl parathion universal antibody and universal envelope antigen

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Embodiment Construction

[0034] The present invention will be further described below with embodiment:

[0035] Artificial antigen preparation: 60mg and 72mg of chlorpyrifos artificial hapten and methyl parathion artificial hapten were dissolved in 5.0ml DMF. Dissolve 0.2 mL of tri-n-butylamine in 5.0 mL of DMF, and add e to d to obtain f. Dissolve 0.16 mL of isobutyl chloroformate in 10 mL of DMF, add dropwise to f at 4°C, and react for 1 hour to obtain g. 120 mg of bovine serum albumin was dissolved in 60 mL of PH=8.7, 0.05 M borate buffer solution, then g was added dropwise to it below 18°C, and reacted at room temperature (~20°C) for 12 hours. The obtained reaction solution was put into a dialysis bag, and dialyzed in PH=7.4, 0.01M buffer solution at 4°C for 60 hours, and the PBS dialysate was changed every 8 hours, 1 L each time. Until no hapten UV absorbance (OD 282 ). Accurately measure the volume of the protein solution after dialysis, determine the protein concentration and binding ratio ...

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Abstract

The invention discloses preparation of chlorpyrifos and methyl parathion common antibody of an enzyme-linked immunoadsorption determination reagent kit suitable for analyzing chlorpyrifos and methyl parathion residue at the same time and common envelope antigen, which comprises preparation methods of chlorpyrifos semiartificial antigen, methyl parathion semiartificial antigen structure, chlorpyrifos and methyl parathion common antibody and common envelope antigen. The chlorpyrifos and methyl parathion common antibody and the common envelope antigen prepared by the preparation methods can be used to prepare the enzyme-linked immunoadsorption determination reagent kit suitable for analyzing chlorpyrifos and methyl parathion residue at the same time; moreover, the reagent kit can be used for quickly detecting chlorpyrifos residue and methyl parathion in water, soil, foodstuff such as vegetables and poisoned samples; in addition, the invention has the advantages of simple preliminary treatment process of samples, simultaneous detection of samples in batches and lower sample detection cost than the prior detection method.

Description

(1) Technical field [0001] The invention belongs to the field of residual pesticide detection, and relates to the preparation of an enzyme-linked immunosorbent assay kit suitable for analyzing residual chlorpyrifos and methyl parathion at the same time, and the preparation of a universal antibody for chlorpyrifos and methyl parathion and a universal coating antigen . (2) Background technology [0002] The traditional residue analysis methods of pesticides and their metabolites mainly rely on physical and chemical analysis methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), and mass spectrometry (MS). The chronic and long-term effects of human health and human health are increasingly concerned and worried, and the restrictions on pesticide residues are becoming more and more stringent. New requirements and higher requirements are put forward for the analysis and measurement objects, types, quantities, ranges, indicators, etc. However, the ...

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Application Information

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IPC IPC(8): G01N33/531
Inventor 孙家隆
Owner 孙家隆
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