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Enzyme linked immunosorbent assay reagent kit for simultaneously analyzing carbaryl and methyl parathion

An enzyme-linked immunosorbent assay and methyl parathion technology, which is used in the analysis of materials, measuring devices, instruments, etc.

Inactive Publication Date: 2009-07-08
孙家隆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there is no technology about convenient simultaneous detection of carbaryl and methyl parathion in the prior art

Method used

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  • Enzyme linked immunosorbent assay reagent kit for simultaneously analyzing carbaryl and methyl parathion
  • Enzyme linked immunosorbent assay reagent kit for simultaneously analyzing carbaryl and methyl parathion
  • Enzyme linked immunosorbent assay reagent kit for simultaneously analyzing carbaryl and methyl parathion

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0072] In the kit, the complex of carbaryl and methylparathion artificial hapten and ovalbumin is used as a general-purpose coating antigen, and the pH is 9.6 with 0.05mol / L carbonate buffer solution (containing 1g sodium carbonate and 4g of sodium bicarbonate, 1L of double distilled water) to 4ug / mL, spotted on a 96-well / 40-well ELISA plate, 100ug / well, and blocked with 5% gelatin.

[0073] Reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl and methyl parathion common antibody (indirect ELISA), carbaryl and methyl parathion standard solution, horseradish peroxidation Phytase-labeled goat anti-rabbit common antibody (suitable for indirect competition ELISA), horseradish peroxidase-labeled anti-carbaryl and methylparathion rabbit common antibody (suitable for direct competition ELISA), substrate...

Embodiment 2

[0082] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0083] Trial basin Jiami uses the complex of carbaryl and methylparathion artificial half-antibody and ovalbumin as a universal coating antigen (CBRH-OVA), which is mixed with 0.05mol / L carbonate with a pH of 9.6 The buffer solution (containing 2g sodium carbonate and 2g sodium bicarbonate, 1L of double distilled water) was diluted to 0.5ug / mL, spotted on a 96-well / 40-well microplate, 100ug / well, and blocked with 5% gelatin.

[0084] Reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl and methyl parathion common antibody (indirect ELISA), carbaryl and methyl parathion standard solution, horseradish peroxidation Phytase-labeled goat anti-rabbit common antibody (suitable for indirect competition ELISA), horseradish peroxidase-labeled anti-carbaryl and methylparathion rabbit common antibody (suitab...

Embodiment 3

[0093] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0094] In the kit, the complex of carbaryl and methylparathion artificial half-antibody and ovalbumin is used as a universal coating antigen, and it is mixed with a 0.05mol / L carbonate buffer solution (containing 2g sodium carbonate) with a pH of 9.6 and 2g sodium bicarbonate, double distilled water 1L) diluted to 0.5ug / ml, spotted on a 96-well / 40-well microplate, 100ug / well, and blocked with 5% gelatin.

[0095] Reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl and methyl parathion common antibody (indirect ELISA), carbaryl and methyl parathion standard solution, horseradish peroxidation Phytase-labeled goat anti-rabbit common antibody (suitable for indirect competition ELISA), horseradish peroxidase-labeled anti-carbaryl and methylparathion rabbit common antibody (suitable for direct competi...

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Abstract

The invention discloses an enzyme-linked immunosorbent assay kit suitable for residue analysis of carbaryl and methyl parathion simultaneously, which comprises a kit body, an ELISA plate / tube and reagents arranged in the kit body. Each hole of the ELISA plate is provided with general coating antigen, wherein the general coating antigen is coated by a coating liquid and can undergo specific reaction with co-anti-antibody against carbaryl and methyl parathion, and is sealed with gelatin; the regents in the kit comprise a washing liquid, a substrate diluent, a standard solution of carbaryl and methyl parathion , antibodies against carbaryl and methyl parathion, enzyme-labeled antibodies against carbaryl and methyl parathion, a substrate, a coloration substance and a reaction terminating solution. The enzyme-linked immunosorbent assay kit can be used to detect carbaryl and methyl parathion residues in food such as water, soil, vegetable and poisoned samples simultaneously and rapidly, has simple pretreatment process of the samples, and detect batch samples simultaneously, and has sample detection cost lower than the prior detection method.

Description

(1) Technical field [0001] The invention relates to an enzyme-linked immunosorbent assay kit suitable for analyzing residual carbaryl and methyl parathion at the same time. The kit is mainly suitable for rapid determination of batches of water samples, soil and other environmental samples and poisoned samples Carbaryl and methyl parathion residues in food samples such as vegetables and vegetables. The invention belongs to the field of residual pesticide detection. (2) Background technology [0002] The traditional residue analysis methods of pesticides and their metabolites mainly rely on physical and chemical analysis methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), and mass spectrometry (MS). The chronic and long-term effects of human health and human health are increasingly concerned and worried, and the restrictions on pesticide residues are becoming more and more stringent. New requirements and higher requirements are put forward ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/535
Inventor 孙家隆
Owner 孙家隆
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