Acetylcholinesterase biosensor and application thereof

A technology of acetylcholinesterase and biosensor, which is applied in the field of electrochemical detection, can solve the problems of surface-enhanced Raman spectroscopy, such as poor reproducibility and stability, expensive instruments, and short optical path, and achieve excellent electrocatalytic activity and biocompatibility performance, short detection time, and high sensitivity

Active Publication Date: 2016-12-21
云南省产品质量监督检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to gas chromatography, high performance liquid chromatography and chromatography-mass spectrometry, the instruments are expensive and difficult to miniaturize, and the sample pretreatment process is relatively complicated; capillary electrophoresis has small capillary diameter, short optical path, and poor reproducibility; surface enhancement Poor reproducibility and stability of Raman spectroscopy

Method used

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  • Acetylcholinesterase biosensor and application thereof
  • Acetylcholinesterase biosensor and application thereof
  • Acetylcholinesterase biosensor and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] First, prepare Pd-Pt@MnO with good dispersion 2 Nanocomposite material: Dissolve 911mg cetyltrimethylammonium bromide in 25mL of ultrapure water, heat and reflux at 140°C, then add KMnO 4 Its mass is 79.02mg dissolved in 25mL of ultra-pure water. When the temperature is kept at 140°C, reflux for 8 to 10 minutes. The color changes from purple to dark brown. Cool to room temperature, wash with absolute ethanol three times, and dilute to 10mL with water. (equivalent to 6mg / mL), that is, to get MnO 2 -nanoflakes material. Take 5mL of the prepared MnO 2 -nanoflakes material, add 0.5mL 10mM PdCl 2 , 0.5mL 10mM H 2 PtCl 6 , 0.1mL PEG400 and 1.0mL 10mM sodium citrate were stirred and mixed, and finally 3mL 15mM sodium borohydride was added to react for 4h, then washed with water three times, centrifuged, and the volume was adjusted to 5mL to obtain Pd-Pt@MnO 2 composite material.

[0038] Take 6uL of Pd-Pt@MnO obtained above 2 The suspension was drop-coated on the surfa...

Embodiment 2

[0041] First, prepare Pd-Pt@MnO with good dispersion 2 Nanocomposite material: Dissolve 911mg of cetyltrimethylammonium bromide in 25mL of ultrapure water, heat to reflux until the temperature is maintained at 140°C, then add KMnO 4 Its mass is 79.02mg, dissolved in 25mL of ultrapure water at 140°C, and refluxed for 8 to 10 minutes when the temperature is kept at 140°C, the color changes from purple to dark brown, cooled to room temperature, washed three times with absolute ethanol, and fixed with water. To 10mL (equivalent to 7mg / mL), that is, get Pd-Pt@MnO 2 composite material.

[0042] Take 6uL obtained Pd-Pt@MnO 2 The suspension was drop-coated on the surface of the pre-treated glassy carbon electrode GCE, and dried at room temperature to obtain Pd-Pt@MnO 2 Modified glassy carbon electrode, denoted as Pd-Pt@MnO 2 / GCE; finally in the dried Pd-Pt@MnO 2 4.5 μL of acetylcholinesterase (AChE) and chitosan (CS) mixture was drop-coated on the surface of the modified glassy ...

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Abstract

The invention relates to an acetylcholinesterase biosensor and an application thereof, and belongs to the technical field of electrochemical detection. The biosensor is produced through the following steps: 1, preparing a MnO2-nanoflakes composite nano-material and a Pd-Pt@MnO2-nanoflakes composite nano-material from KMnO4, CTAB, PdCl2, PEG400, H2PtCl6, sodium citrate and sodium borohydride used as initial raw materials through a water bath technology; and 2, immobilizing acetylcholinesterase by using chitosan (CS), and immobilizing acetylcholinesterase (AChE) on the surface of a Pd-Pt@MnO2 modified glassy carbon electrode through adopting physical adsorption to construct the electrochemical biosensor. The biosensor adopting an electrochemistry and enzyme sensing combination technology realizes high-sensitivity detection of methyl parathion and carbofuran pesticides, has the advantages of small amount of a required sample, short detection time, high sensitivity and low cost, and is suitable for analyzing and detecting pesticide residual.

Description

technical field [0001] The invention relates to an acetylcholinesterase biosensor and its application, in particular to an electrochemical biosensor based on a platinum-palladium alloy loaded manganese dioxide nanocomposite material and its application, and belongs to the technical field of electrochemical detection. Background technique [0002] As a special means of production in the process of agricultural production, pesticides can eliminate pests, increase yields, ensure harvests, and prevent and control human infectious diseases. However, the food safety problems caused by the extensive use of pesticides are also becoming more and more prominent. Pesticides enter the human body through contaminated agricultural products, and people will be poisoned. Some harmful elements may cause human cells to become cancerous, and even affect the health of future generations. Therefore, in order to improve and ensure food quality and life safety, highly sensitive detection of pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3277
Inventor 于毅涛李灿鹏杨龙张艳琼马雪涛邱烨
Owner 云南省产品质量监督检验研究院
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