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Luminol-luminescent functionalized nano-silver as well as preparation method and application for same

A nano-silver, functionalized technology, applied in the field of biochemistry, analytical chemistry and medicine, material science

Active Publication Date: 2012-07-11
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical and chemical properties of nano silver are different from that of nano gold, it has better chemical activity and catalytic properties, but there is no literature report on luminol functionalized nano silver and its application in biological analysis

Method used

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  • Luminol-luminescent functionalized nano-silver as well as preparation method and application for same
  • Luminol-luminescent functionalized nano-silver as well as preparation method and application for same
  • Luminol-luminescent functionalized nano-silver as well as preparation method and application for same

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preparation example Construction

[0144] 2. the preparation method of functionalized nano-silver of the present invention, comprises the steps:

[0145] (1) Mix water and absolute ethanol under stirring; the volume of the water is 0.5-6 times of the volume of the absolute ethanol; then add silver nitrate aqueous solution under stirring to form a mixed solution; the nitric acid The mol ratio of silver and described dehydrated alcohol is 1: 1.5 * 10 6 -1:1.4×10 7 ;

[0146] (2) In the stirring state, add the luminol aqueous solution to the mixed solution obtained in step (1), wherein the molar ratio of silver nitrate to luminol is 1-20:1, and continue to stir until the silver sol form is formed. silver nanoparticles.

[0147] The molar concentration of the silver nitrate aqueous solution can be 1-10mmol / L, 1-5mmol / L, 5-10mmol / L, preferably 5mmol / L;

[0148] The molar concentration of the luminol aqueous solution can be 1-20mmol / L, 1-5mmol / L, 5-10mmol / L, preferably 10mmol / L.

[0149] The molar ratio of silve...

Embodiment 1

[0203] Embodiment 1: The influence of the concentration of silver nitrate in synthesizing luminol light-emitting functionalized nano-silver

[0204] Mix 10mL 1.0mmol / L silver nitrate aqueous solution, 9mL absolute ethanol and 5mL ultrapure water (resistivity 18.2MΩ*cm) under stirring at 25°C, add 0.5mL 0.01mol / L luminol, and continue stirring for 2 hours Finally, a stable luminol-luminescent functionalized nano-silver sol was obtained, which was stored at 4° C. in the dark. The silver sol was characterized by electron microscopy through centrifugal purification. The purification conditions are as follows: centrifuge 1ml of the silver sol at 12500r / min for 45 minutes, then dissolve the flocs with absolute ethanol to obtain purified functionalized silver nanoparticles, and carry out electron microscope characterization of the silver sol by centrifugal purification . The results show that the morphology of the synthesized luminol luminescent functionalized silver nanoparticles ...

Embodiment 2

[0208] Example 2: Influence of the amount of silver nitrate on the particle size in the synthesis method of luminol luminescent functionalized nano silver

[0209] Mix 2mL 5.0mmol / L silver nitrate aqueous solution, 9mL absolute ethanol and 5mL ultrapure water (resistivity 18.2MΩ*cm) under stirring at 25°C, add 0.5mL 0.01mol / L luminol, and continue stirring for 2 hours Finally, a stable luminol-luminescent functionalized nano-silver sol was obtained, which was stored at 4° C. in the dark. The silver sol was characterized by electron microscopy through centrifugal purification. The purification conditions are as follows: centrifuge 1ml of the silver sol at 12500r / min for 45 minutes, then dissolve the flocs with absolute ethanol to obtain purified functionalized nano-silver, and perform electron microscopy on the silver sol by centrifugal purification. characterization. The results show that the morphology of the synthesized luminol luminescent functionalized silver nanoparticl...

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Abstract

The invention discloses a luminol-luminescent functionalized nano-silver as well as a preparation method and an application for the same. The invention discloses a functionalized nano-silver, formed by connecting silver nanoparticles with luminol, wherein the luminol is connected to the surfaces of the silver nanoparticles via a covalent bond Ag-N. In the preparation method, nano-silver is prepared by directly reducing silver nitrate by luminol in the presence of ethanol, and luminol is used as a reducing agent and a protecting reagent simultaneously during synthesis process. The method has the advantages of being simple, fast, cheap and the like, as well as is a new technology for synthesising nano-silver at a temperature ranging from 25 to 40 DEG C. The particle size and shape of the nano-silver synthesised by the method can be regulated and controlled by the ratio of silver nitrate to luminol, and the obtained nano-silver has excellent chemiluminescent characteristic. The invention further discloses a bioanalysis probe based on the luminescent functionalized nano-silver, as well as an analysis method based on the probe and a kit. The functionalized nano-silver, the bioanalysis probe thereof, the analysis method and the kit disclosed by the invention can be used in the fields of immunoassay, nucleic acid analysis, molecular imaging, sensor etc.

Description

technical field [0001] The invention relates to luminol luminescent functionalized nano-silver, its preparation and properties, biological probes (nucleic acid probes and immune probes) using the functionalized nano-silver, a biological analysis method and a kit. The invention belongs to the fields of material science, biochemistry, analytical chemistry and medicine. Background technique [0002] Due to the surface effect, small size effect and quantum size effect, nanoscale silver presents unique optical, low toxicity, electrical and thermal properties different from its macroscopic bulk, and at the same time has the easy surface modification of its macroscopic bulk The chemical properties make it have important application prospects in catalysis, sensing, optoelectronics and other fields. A large number of experimental and theoretical studies have shown that the properties of silver nanostructures are not only affected by size, but also closely related to their shape and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/07C12N15/11C12Q1/68G01N21/76G01N33/543
Inventor 崔华何毅
Owner UNIV OF SCI & TECH OF CHINA
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