Chemiluminescent substrate solution

A technology of chemiluminescence and substrate solution, which is applied in the direction of chemiluminescence/bioluminescence, luminescent materials, chemical instruments and methods, etc., can solve the problems of test signal value drop, narrow detection range, inconvenient clinical application, etc., and achieve simple operation , low cost, non-toxic and harmless to the human body

Active Publication Date: 2011-06-01
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional chemiluminescent substrate solution is composed of A liquid (oxidant) and B liquid (luminescent agent). A liquid and B liquid are stored separately. Use it up, otherwise its stability will deteriorate, causing the test signal value to drop sharply, the detection range will be significantly narrowed, and the stability will deteriorate, which is not convenient for clinical application

Method used

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Embodiment 1

[0015] The anti-thyroid-stimulating hormone (TSH) chemiluminescent immunoassay method established with the one-component chemiluminescent substrate solution prepared by the embodiments of the present invention comprises the following steps:

[0016] Step 1, prepare chemiluminescence substrate solution, its composition and concentration are:

[0017] 0.1mol / L Tris buffer system pH 9.0

[0018] Luminol 0.2g / L

[0019] Cinnamic acid 0.1g / L

[0020] 4-iodophenylboronic acid 0.05g / L

[0021] p-iodophenol 0.05g / L

[0022] Carbamide peroxide 0.1g / L

[0023] Dimethylformamide (DMF) 5ml / L

[0024] Polyvinyl alcohol (PVA) 2.5g / L

[0025] Polyvinylpyrrolidone (PVP) 5g / L

[0026] Polyethylene glycol 600 (PEG-600) 1.5g / L

[0027] Ethylenediaminetetraacetic acid (EDTA) 1g / L

[0028] Gentamicin sulfate 400,000 units / L

[0029] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours....

Embodiment 2

[0038] The anti-thyroid-stimulating hormone (TSH) chemiluminescent immunoassay method established with the one-component chemiluminescent substrate solution prepared by the embodiments of the present invention comprises the following steps:

[0039] Step 1, prepare chemiluminescence substrate solution, its composition and concentration are:

[0040] 0.1mol / L Tris buffer system pH 9.0

[0041] Luminol 0.7g / L

[0042] Cinnamic acid 0.9g / L

[0043] 4-iodophenylboronic acid 0.2g / L

[0044] p-iodophenol 0.25g / L

[0045] Carbamide peroxide 0.4g / L

[0046] Dimethylformamide (DMF) 25ml / L

[0047] Polyvinyl alcohol (PVA) 5g / L

[0048] Polyvinylpyrrolidone (PVP) 8g / L

[0049] Polyethylene glycol 600 (PEG-600) 3g / L

[0050] Ethylenediaminetetraacetic acid (EDTA) 4g / L

[0051] Gentamicin sulfate 1.6 million units / L

[0052] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours....

Embodiment 3

[0067] The anti-thyroid-stimulating hormone (TSH) chemiluminescent immunoassay method established with the one-component chemiluminescent substrate solution prepared by the embodiments of the present invention comprises the following steps:

[0068] Step 1, prepare chemiluminescence substrate solution, its composition and concentration are:

[0069] 0.1mol / L Tris buffer system pH 9.0

[0070] Luminol 1.2g / L

[0071] Cinnamic acid 5g / L

[0072] 4-iodophenylboronic acid 0.6g / L

[0073] p-iodophenol 0.6g / L

[0074] Carbamide peroxide 1g / L

[0075] Dimethylformamide (DMF) 50ml / L

[0076] Polyvinyl alcohol (PVA) 7.5g / L

[0077] Polyvinylpyrrolidone (PVP) 15g / L

[0078] Polyethylene glycol 600 (PEG-600) 5g / L

[0079] Ethylenediaminetetraacetic acid (EDTA) 10g / L

[0080] Gentamicin sulfate 4 million units / L

[0081] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. Af...

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Abstract

The invention provides a chemiluminescent substrate solution, which comprises the following components: 0.2-1.2g/L of Luminol, 0.1-5g/L of cinnamic acid, 0.05-0.6g/L of 4-iodophenylboronic acid, 0.05-0.6 g/L of p-iodophenol, 5-50ml/L of dimethylformamide (DMF), 2.5-7.5 g/L of polyvinyl alcohol (PVA), 5-15g/L of polyvinyl pyrrolidone (PVP), 1.5-5g/L of polyethylene glycol 600 (PEC -600), 1-10g/L of ethylene diamine tetraacetic acid (EDTA), 400,000-4,000,000 unit/L of gentamicin sulfate, 0.1-1.0g/L of carbamide peroxide and 0.1mol/L of tris buffer solution with a pH vale being 9.0. The chemiluminescent substrate solution is a solution which can be directly used, and maintains wide linear detection range, good stability and high sensitivity, thus solving the problem that a traditional chemiluminescent substrate solution consists of a solution A and a solution B which are stored separately; and after the solution A and the solution B are isovolumetrically mixed for 10 minutes, signal values are decreased sharply, the detection range is narrowed and the stability is poor. In addition, the chemiluminescent substrate solution provided by the invention is easy to prepare, low in cost and simple to operate, environmentally friendly, and non-toxic and harmless to human bodies and the like.

Description

technical field [0001] The invention relates to a chemiluminescent substrate liquid, in particular to a single-component chemiluminescent substrate liquid. Background technique [0002] At present, the most widely used quantitative immunoassay method in the clinical field is mainly chemiluminescence immunoassay (CLIA), which is a sensitive micro-immunoassay, which has all the advantages of (RIA) and EIA, and at the same time overcomes the advantages of radioimmunoassay and enzyme It is the most ideal method for clinical immunoassay due to their respective shortcomings. CLIA involves a key technology, which is to prepare a chemiluminescent substrate solution with high sensitivity, wide detection range and good stability. [0003] The traditional chemiluminescent substrate solution is composed of A liquid (oxidant) and B liquid (luminescent agent). A liquid and B liquid are stored separately. Otherwise, its stability will deteriorate, causing its test signal value to drop sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/577G01N33/53G01N21/76C09K11/06
Inventor 刘萍栾大伟
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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