Preparation method and applications of miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide

A technology of molybdenum disulfide, luminescent immunity, applied in the field of functional materials and biosensing
CN106568936AActive Publication Date: 2017-04-19NINGBO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO UNIV
Publication Date
2017-04-19

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Abstract

The invention discloses a preparation method and applications of a miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide. The preparation method is characterized by comprising: carrying out amination on prepared Fe3O4 magnetic nanoparticles, and reacting with chloroauric acid to prepare Fe3O4@Au magnetic nanoparticles; carrying out carboxylation on prepared monolithic layer MoS2, sequentially reacting with a coupling reagent, a signal DNA solution and a luminol solution, finally adding a mercaptohexanol solution, and carrying out vibration washing to obtain a signal unit solution; carrying out a reaction on the Fe3O4@Au and a DNA capturing solution to obtain a capture unit solution; and coating the capture unit solution on the surface of a magnetic glassy carbon electrode in a dropwise manner, and then sequentially coating miRNA-21 and the signal unit solution on the surface of the magnetic glassy carbon electrode in a dropwise manner so as to obtain the product. The miRNA-21 electroluminescent immunosensor of the present invention has advantages of high sensitivity, strong specificity, high accuracy, simple operation step, and low experimental cost.
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Description

technical field

[0001] The present invention relates to an electrochemiluminescence immunosensor and a detection method thereof, in particular to a preparation method and application of a miRNA-21 electrochemiluminescence immunosensor based on a multifunctional molybdenum disulfide material, belonging to functional materials and biological sensors. technology field. Background technique

[0002] Cancer, also known as malignant tumor, is essentially a polygenic disease caused by the abnormal mechanism of cell growth and proliferation, which seriously endangers human health. According to WHO reports and predictions, cancer cases worldwide will show a rapid growth trend, from 14 million in 2012 to 19 million in 2025 and 24 million in 2035. Oncologists believe that early detection, early diagnosis, and early treatment of tumors can effectively prevent cancer, and early diagnosis is the key. The discovery of tumor markers makes early diagnosis possible. Tumor markers are a cla...

Claims

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