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Method for detecting glucose by ferroferric oxide nano particle catalytic chemiluminescence

A technology of chemiluminescence detection and ferroferric oxide, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of non-recyclable, expensive peroxidase, and poor stability, and achieve The effect of low cost, low detection limit and wide linear range

Inactive Publication Date: 2011-05-11
INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0004] At present, chemiluminescent reactions usually use metal complexes and peroxidases as catalysts to catalyze Luminol-H 2 o 2 The system realizes the detection of glucose [Kricka LJ, Voyta JC, Bronstein I. Chemiluminescent methods for detecting and quantitating enzyme activity. Methods Enzymol, 2000, 305, 370-390.], however, metal complexes easily interfere with the chemiluminescent signal , while peroxidase is expensive, less stable and cannot be recovered [Huang Yuwen, Feng Manliang, Zhang Zhujun

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  • Method for detecting glucose by ferroferric oxide nano particle catalytic chemiluminescence

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Embodiment

[0024] 1) pH optimization

[0025] Preparation of Fe3O4 nanoparticles-Luminol-H 2 O 2 Solution system, in constant iron tetroxide nanoparticles, luminol and H 2 O 2 Concentration and reaction temperature (the concentration of ferroferric oxide nanoparticles is 10ppm; the concentration of luminol is 2mmol / L; H 2 O 2 Under the condition that the concentration is 50μmol / L; the reaction temperature is 25℃), the pH value of the system is adjusted by adding HCl and NaOH to the solution (pH value range is 4-10). After measuring the pH value with a pH meter, use SpectraMaxM2 microplate reader measures the luminous intensity of each system, and the results are as follows figure 1 Shown. It can be seen that when the pH value is in the range of 4-9, the luminous intensity is greater, and when the pH=7.0, the luminous intensity is the highest.

[0026] 2) Temperature optimization

[0027] Preparation of Fe3O4 nanoparticles-Luminol-H 2 O 2 Solution system, in constant iron tetroxide nanoparticle...

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Abstract

The invention discloses a method for detecting glucose by ferroferric oxide nano particle catalytic chemiluminescence, which comprises the following steps of: at the temperature of between 10 DEG C and 60 DEG C, adding a glucose solution into a ferroferric oxide nano granule-luminol-H2O2 solution system, the pH of which is 4.0 to 9.0; and detecting the concentration of the glucose by using a SpectraMaxM2 microplate reader, wherein the concentration of the ferroferric oxide nano particles is more than or equal to 10ppm. The average granule diameter of the ferroferric oxide nano particles is 25nanometers. By using the characteristics of high adsorption, biocompatibility, zymoid catalytic property and reuse of the ferroferric oxide nano particles, the glucose chemiluminescence detection method with simple operation, low cost, wide linear range and low detection limit is established to realize simple, convenient and high-sensitivity detection of the glucose content in the fields of clinic and food.

Description

Technical field [0001] The invention relates to a method for detecting glucose content, in particular to a method for detecting glucose content by chemiluminescence method catalyzed by ferroferric oxide nanoparticles. Background technique [0002] Glucose is the main energy source of human cells. Abnormal changes in its level can directly reflect the body's metabolic disorders. Clinical tests can be used to diagnose diabetes, cell tumors, tuberculosis, meningitis, etc. by measuring the glucose content in human blood, urine, and cerebrospinal fluid Various diseases. In addition, the detection of glucose content in various foods such as juice, honey and wine is an important indicator to measure whether various foods meet the standards. Therefore, rapid quantitative detection of glucose is of great significance in the fields of biochemistry, clinical chemistry and food industry. [0003] At present, the detection methods of glucose mainly include spectrophotometry, electrochemical m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 汪冰丰伟悦杜崇磊杜伟王萌赵宇亮
Owner INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI
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