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Biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence

A cation exchange and nanoparticle technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems that sensors cannot be recycled, biological samples are destroyed, and luminol chemiluminescence is unfavorable.

Inactive Publication Date: 2015-05-27
QINGDAO UNIV OF SCI & TECH +1
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Problems solved by technology

The acid-soluble method generally uses concentrated nitric acid. On the one hand, the addition of concentrated nitric acid will reduce the pH value of the system, which is not conducive to the chemiluminescence of luminol; on the other hand, strong acid will damage biological samples, so that the sensor cannot be recycled.

Method used

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  • Biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence
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  • Biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence

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Experimental program
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Embodiment 1

[0021] 1. Synthesis of CuS nanoparticles

[0022] CuS nanoparticles modified with mercaptopropionic acid were prepared according to literature (Anal. Chem. 2008, 80, 7206-7212). Add 15 μL of mercaptopropionic acid to 50 mL of 0.4 mM Cu(NO 3 ) 2 In the solution, the pH value of the mixed solution was adjusted to 7.0 with 0.5M NaOH solution. Access to N 2 After deoxygenation for 30 min, the N 2 Deoxygenated 50mL of 1.34mM Na 2 S solution was added dropwise to the mixed solution. Response to N 2 Under protection, the reaction was stirred in a water bath at 28° C. for 24 h. During the reaction, the solution gradually changed from brown to dark green. The CuS nano-sol was dialyzed in a dialysis bag with a molecular weight cut-off of 7000 Dollon for 48 hours in the dark, and stored at 4°C for future use.

[0023] 2. Preparation of DNA probes modified by CuS nanoparticles

[0024] In a 10mL small beaker, add 2.0×10 -6 2 mL of DNA probe solution modified with amino group at ...

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Abstract

The invention discloses a biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence. Copper sulphide nano-particles are marked on biological molecules, and a composite structure marked with copper sulphide is obtained through a biological identification function; silver nitrate is added into a system, cation exchange reaction on silver nitrate and copper sulfide nano-particles is instantly carried out so as to generate copper ions; a target is quantitatively detected by utilizing chemiluminescence caused by catalyzing a luminol-hydrogen oxide system by the generated copper ions.

Description

technical field [0001] Copper ions can catalyze the chemiluminescence of the luminol-hydrogen peroxide system. The present invention uses the cation exchange reaction between silver ions and copper sulfide nanoparticles to instantly release a large amount of copper ions, and catalyzes the chemical luminescence of the luminol-hydrogen peroxide system through the copper ions. Luminous, to achieve the detection of the target. Background technique [0002] Chemiluminescence analysis is a trace analysis method that determines the content of substances according to the light radiation intensity generated by chemical reactions. The outstanding advantage of chemiluminescence analysis is that it does not use any external light source, thereby avoiding the interference of Rayleigh scattering and Raman scattering. The signal-to-noise ratio is greatly improved, so chemiluminescence analysis generally has high sensitivity. At the same time, chemiluminescence analysis instruments also ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 张晓茹张园刘洪霞
Owner QINGDAO UNIV OF SCI & TECH
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