Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates

A barium tungstate and line array technology, applied in nanotechnology, nanotechnology, nanostructure manufacturing, etc., can solve the problems of high cost, complicated reaction process, and hindering the application of BaWO4, and achieve low cost, simple process, and excellent stimulation. Effect of Raman characteristics

Inactive Publication Date: 2010-08-18
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that the reaction process is complicated and the cost is high, which hinders the BaWO 4 Applications

Method used

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  • Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates
  • Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates
  • Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates

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Experimental program
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Effect test

Embodiment 1

[0019] 100 μl of Escherichia coli genomic DNA solution with a concentration of 1.03 μg / μL and an optical density (OD) value of 1.78 was thoroughly mixed to completely disperse the DNA in deionized water. Then add 5mM Ba(NO 3 ) 2 100 μl of the solution was mixed thoroughly, and incubated at 4°C for 48 hours on a shaker at 80 rpm. Add 5 mM Na to the above mixed solution 2 WO 4 Solution 100μl, mix well. Incubate for 48 hours at 4°C with shaking on a shaker at 80 rpm. Finally, the above mixed solution was placed in a constant temperature metal bath at 80°C and heated for 8h.

Embodiment 2

[0021] 100 μl of Escherichia coli genomic DNA solution with a concentration of 2.10 μg / μL and an optical density (OD) value of 1.81 was thoroughly mixed to completely disperse the DNA in deionized water. Then add 10mM Ba(NO 3 ) 2 100 μl of the solution was mixed thoroughly, and then incubated at 5° C. on a shaker at 85 rpm for 60 h. Add 10 mM Na to the above mixed solution 2 WO 4 Solution 100μl, mix well. Incubate for 60 h at 5°C with shaking on a shaker at 85 rpm. Finally, the above mixed solution was placed in a constant temperature metal bath at 80°C and heated for 7h.

Embodiment 3

[0023] 200 μl of Escherichia coli genomic DNA solution with a concentration of 1.60 μg / μL and an optical density (OD) value of 1.79 was thoroughly mixed to completely disperse the DNA in deionized water. Then add 5mM Ba(NO 3 ) 2 200 μl of the solution was mixed thoroughly, and then incubated at 6° C. on a shaker at 90 rpm for 72 hours. Add 5 mM Na to the above mixed solution 2 WO 4 Solution 200μl, mix well. Incubate for 72 hours at 6°C with shaking on a shaker at 90 rpm. Finally, the above mixed solution was placed in a constant temperature metal bath at 85°C and heated for 6h.

[0024] figure 1 It is the transmission electron microscope photo of the barium tungstate nano double line array prepared in embodiment 1, as can be seen from the figure, the prepared barium tungstate is a good nano double line array shape, because the sample has not been negatively dyed but Directly detected under the transmission electron microscope, it can be proved that the barium tungstate ...

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Abstract

The invention discloses a method for preparing barium tungstate nanometer double-line arrays by using colon bacillus genome DNA as templates, which belongs to the technical field of nanometer materials. The method comprises the following steps: adding a barium nitrate solution into a colon bacillus genome DNA solution; uniformly mixing the solution; carrying out oscillation hatching for 48 to 72 h under the conditions of the temperature between 4 and 6 DEG C and the oscillation speed between 80 and 90 r/min; and then, adding the sodium tungstate solution for oscillation hatching for 48 to 72 h under the conditions of the temperature between 4 and 6 DEG C and the oscillation speed between 80 and 90 r/min; . When the mixed solution is heated for 6 to 8 h under the condition of the temperature between 80 and 85 DEG C, the barium tungstate nanometer double-line arrays using the colon bacillus genome DNA as templates can be obtained. The invention avoids the complicated preparation process of the conventional method, can be completed under the mild conditions of low temperature, normal pressure and the like, the technology is simple, the cost is low, and the reaction can be easily controlled, so the goal of using the colon bacillus genome DNA as templates for synthesizing the barium tungstate nanometer double-line arrays to construct nanometer devices can be realized.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a method for preparing barium tungstate nanometer double-line arrays based on Escherichia coli genome DNA as a template. Background technique [0002] BaWO with scheelite structure (tetragonal system) 4 Crystal is a promising Raman laser crystal with interesting properties such as photoluminescence, thermoluminescence and stimulated Raman scattering (SRS), and has important applications in the fields of optoelectronics, medicine and spectroscopy. value. In 1999, Russia's Basiev et al. measured the Raman spectra of tungstate and other types of crystals and found that BaWO 4 The Raman spectrum of the crystal has a large Raman scattering cross section and Raman scattering intensity, so BaWO 4 The crystal has a large Raman gain no matter under the action of nanosecond pulse or picosecond pulse laser, pointing out that BaWO 4 Crystal is a very promising Raman crystal. wxya ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G41/00B82B3/00
Inventor 高发明李娜侯莉
Owner YANSHAN UNIV
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