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Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

a technology of asymmetric pulsed field electrophoresis and macromolecules, which is applied in the direction of electrostatic separators, diaphragms, electrolysis, etc., can solve the problem that the standard method only works effectively, and achieve the effect of quick fractionation of charged macromolecules

Inactive Publication Date: 2005-07-28
THE TRUSTEES FOR PRINCETON UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

"The present invention provides a method and apparatus for quickly and continuously fractionating charged macro-molecules, such as DNA, using asymmetric pulsed electrophoresis. The method involves applying an alternating electric field with two different orientations and one field being stronger in duration or intensity. The DNA molecules are then fractionated based on size and driven to a far side of the matrix where they are recovered. The apparatus includes a matrix with obstacles and a power supply for applying the alternating electric field. The technical effects of this invention include faster and more efficient fractionation of charged macro-molecules and continuous operation."

Problems solved by technology

However, this standard method only works effectively for DNA molecules smaller than 40 kbp.

Method used

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  • Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
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  • Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

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Embodiment Construction

[0020] The present invention relates to a method and apparatus for fractionation of charged macro-molecules such as DNA. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to size and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

[0021] The present invention provides a method and apparatus for the fractionation of macro-molecules on micro / nano-fabricated support materials (a.k.a. matrices). Because the motion of DNA molecules can be accurately controlled in micro / nano-fabricated environments, the fractionation of DNA can be achie...

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Abstract

A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

Description

RELATED APPLICATIONS [0001] This application claims the priority of Provisional Application Ser. No. 60 / 256,298, filed Dec. 18, 2000, the entire disclosure of which is expressly incorporated herein by reference.GOVERNMENT RIGHTS [0002] The present invention has been made under Federal Contract Grant No. MDA 972-00-1-0031 and the government may have certain rights to the subject invention.BACKGROUND OF THE INVENTION [0003] 1. Field of the Invention [0004] The present invention relates to a method and apparatus for fractionating charged macro-molecules such as DNA using asymmetric pulsed field electrophoresis. [0005] 2. Related Art [0006] The analysis and fractionation of large DNA molecules is a central step in large scale sequencing projects. Conventionally, gel electrophoresis is used to fractionate DNA molecules according to their sizes. This method includes two steps: sample loading and fractionation. First, sample solution containing DNA is loaded into loading wells in the gel s...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01L3/00C07H21/04C12N15/10G01N27/447
CPCC12N15/101Y10T436/25Y10T436/11Y10T436/10
Inventor HUANG, LOTIEN RICHARDSTURM, JAMES CHRISTOPHERAUSTIN, ROBERT HAMILTON
Owner THE TRUSTEES FOR PRINCETON UNIV
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