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Fractionation of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis

a technology of asymmetric pulsed field electrophoresis and macromolecules, which is applied in the direction of fluid pressure measurement, liquid/fluent solid measurement, peptide measurement, etc., can solve the problem that the standard method only works effectively, and achieve the effect of quick fractionation of charged macromolecules

Inactive Publication Date: 2009-01-15
THE TRUSTEES OF PRINCETON UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]It is an object of the present invention to provide a method and apparatus for quickly fractionating charged macro-molecules.

Problems solved by technology

However, this standard method only works effectively for DNA molecules smaller than 40 kbp.

Method used

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Embodiment Construction

[0020]The present invention relates to a method and apparatus for fractionation of charged macro-molecules such as DNA. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to size and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

[0021]The present invention provides a method and apparatus for the fractionation of macro-molecules on micro / nano-fabricated support materials (a.k.a. matrices). Because the motion of DNA molecules can be accurately controlled in micro / nano-fabricated environments, the fractionation of DNA can be achieved...

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Abstract

A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

Description

RELATED APPLICATIONS[0001]This application claims the priority of Provisional Application Ser. No. 60 / 256,298, filed Dec. 18, 2000, the entire disclosure of which is expressly incorporated herein by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates to a method and apparatus for fractionating charged macro-molecules such as DNA using asymmetric pulsed field electrophoresis.[0004]2. Related Art[0005]The analysis and fractionation of large DNA molecules is a central step in large scale sequencing projects. Conventionally, gel electrophoresis is used to fractionate DNA molecules according to their sizes. This method includes two steps: sample loading and fractionation. First sample solution containing DNA is loaded into loading wells in the gel slab before the electric field is turned on. Then, an electric field is applied. The DNA molecules move in the opposite direction of the electric field because they are negatively charged. As ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01D57/02B01L3/00C12N15/10C07H21/04G01N27/447
CPCC12N15/101Y10T436/25Y10T436/11Y10T436/10
Inventor HUANG, LOTIEN RICHARDSTURM, JAMES CHRISTOPHERAUSTIN, ROBERT HAMILTON
Owner THE TRUSTEES OF PRINCETON UNIV
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