Detection method of substances relative to raw material and preparation of ambrisentan
An ambrisentan and detection method technology, which is applied in the field of drug analysis, can solve problems such as research and elaboration of the detection method of raw materials and preparations without ambrisentan, and research on the detection method of substances, so as to achieve accurate and credible detection results and suitable retention time. , special effect
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Embodiment 1
[0065] Detection method for related substances of ambrisentan raw material of the present invention
[0066] 1. Testing instruments and testing conditions
[0067] Instrument: Waters e2695; Waters2489 UV detector
[0068] Chromatographic column: C18 column (5μ, 4.6×250mm)
[0069] Mobile phase: A: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 45: 55 (v / v);
[0070] B: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 70:30 (v / v);
[0071] Flow rate: 1ml / min; detection wavelength: 220nm;
[0072] Column temperature: 30°C; automatic sample chamber temperature is 4°C.
[0073] 2. Solution preparation: take the ambrisentan raw material sample, add mobile phase A to dissolve and make the test solution containing about 0.5mg of ambrisentan in every 1ml.
[0074] Precisely measure 1ml of the test solution, put it in a 100ml volumetric flask, dilute to the mark with mobile phase A, shake well, and u...
Embodiment 2
[0084] Detection method for related substances of ambrisentan preparation of the present invention
[0085] 1. Testing instruments and testing conditions
[0086] Instrument: Waters e2695; Waters2489 UV detector
[0087] Chromatographic column: C18 column (5μ, 4.6×250mm)
[0088] Mobile phase: A: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 45: 55 (v / v)
[0089] B: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 70:30 (v / v)
[0090] Flow rate: 1ml / min; detection wavelength: 220nm;
[0091] Column temperature: 30°C; automatic sample chamber temperature is 25°C;
[0092] 2. Solution preparation: take 10 ambrisentan tablets, grind them evenly in a mortar, accurately weigh the tablet powder, put it in a volumetric flask, add mobile phase A for ultrasonic dissolution, dilute to the mark with mobile phase A for cooling, and measure by centrifugation. The supernatant was made into a test solutio...
Embodiment 3
[0100] Detection method for related substances of ambrisentan raw material of the present invention
[0101] 1. Testing instruments and testing conditions
[0102] Instrument: Waters e2695; Waters2489 UV detector
[0103] Chromatographic column: C8 column (5μ, 4.6×250mm)
[0104] Mobile phase: A: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 45: 55 (v / v);
[0105] B: Acetonitrile: 30mM potassium dihydrogen phosphate (phosphoric acid adjusted to pH 3.5) = 70:30 (v / v);
[0106] Flow rate: 1ml / min; detection wavelength: 220nm;
[0107] Column temperature: 30°C; automatic sample chamber temperature is 4°C.
[0108] 2. Solution preparation: take the ambrisentan raw material sample, add mobile phase A to dissolve and make the test solution containing about 0.5mg of ambrisentan in every 1ml.
[0109] Precisely measure 1ml of the test solution, put it in a 100ml volumetric flask, dilute to the mark with mobile phase A, shake well, and us...
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